VALIDATED FIRST-ORDER DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM

  • P G Sunitha Department of Pharmaceutical Chemistry,College of Pharmacy, Madras Medical College, Chennai-600 003.
  • Ilango Kaliappan Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM University, Kattankulathur-603 203.

Abstract

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of emtricitabine(EMB) and tenofovir(TEN) by employing first-order derivative spectrophotometric method. The first derivative spectrum is a plot of the rate of change of absorbance with wavelength against wavelength (dA /dλ versus λ). It is characterized by a maximum, minimum and a cross- over point at the λmax of the absorption band. The linearity was established over the concentration range of 6-16 μg/mL and 9-24 μg/mL for EMB and TEN with correlation coefficients 0.9994and 0.9992, respectively. The mean percentage recoveries were found to be 99.99% for EMB and 100.43%for TEN. The proposed method has been validated as per ICH guidelines and successfully applied for the simultaneous estimation of EMB and TEN in combined tablet dosage form.

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Sunitha P, Kaliappan I. VALIDATED FIRST-ORDER DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM. JDDT [Internet]. 14Mar.2014 [cited 30Nov.2020];4(2):9-1. Available from: http://jddtonline.info/index.php/jddt/article/view/763