COMPARATIVE EFFICACY OF CLOVE OIL AND 2-PHENOXY ETHANOL ON SERUM BIOCHEMICAL CHANGES AND HISTOLOGICAL STUDIES IN CHANNAPUNCTATUS
The aim of the present study was to investigate thecomparison of effect of clove oil and 2-phenoxy ethanol for Channapunctatus and using values of serum biochemical profile and histological tissue studies ,to assess the effects of fish exposure to that anaesthetic:A total of 60 C.punctatus of 60.23Â±5.02g were divided in to five groups (12 fish per each):1st treatment as control (no anaesthetic),2nd 0.2ml/1 2-phenoxy ethanol ,3rd 0.3ml/1 2-phenoxy ethanol ,4th 30mg/1 clove oil and 5th 40mg/1 clove oil.Biochemical blood profile of C.puntatus were taken 15 min and 24 hrs. After Anaesthesia induction ,the factors used to evaluate the serum biochemical profile included the glucose (GLU), total protein (TP), albumin (ALB),total globulin (GLOP), alkaline phosphatase (ALP),serum Glutamic oxaloacetic Transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), histological tissue examination of tissue like gills ,ten minutes exposure to 2-phenoxy ethanol and clove oilcaused an increase in concentration of glucose and alkaline phosphatase 15 min after Anaesthesiainduction.Histological examination showed.Capillary ectasia of gill filaments immediately after clove oil and 2-phenoxy ethanol,showedÂ swelling of primary and secondary lamella .Twenty â€“four hours after anaesthesia ,no ectasia was observed .No histopathological changes were demonstrated in gills:Our result showed that The 2-phenoxy ethanol at 0.20ml/1and clove oil at 30mg/1 concentration may be used as an efficient and safe anaesthetic for Channapunctatus.
Key words: Clove oil, 2-Phenoxy ethanol,Channapunctaus,Serum biochemical profile, histological examination of tissues.
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