Frequency and Distribution of Fok1rs 2228570 of Vitamin D Receptor Gene among Healthy Sudanese Population

  • Amira S. Khalafalla Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan
  • Yousif A. Mohammed National Cancer Institute, University of Gezira, Sudan
  • Adam D. Abakar Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan
  • A Mergani Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan
  • Amer M. Dafaalla Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan
  • Mohammed Abdelwahed Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan
  • Omer Balla Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan
  • Mutaz I. Hassan Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Shendi University, Sudan

Abstract

Background: Vitamin D plays a role in critical cellular processes such as apoptosis, cell development, and division. The Fok1 polymorphism in the VRD gene is T/C resulting in larger vitamin D receptor protein with lesser expression activity. Fok1 polymorphism is thought to be linked to many diseases including diabetes mellitus, cancers, and rheumatoid arthritis. The snipe also provides effective and sensitive diagnostic and predictive tools and affected by differences in ethnicity and individuals' life style. This study reports on the frequency and distribution of Fok1 SNP among healthy Sudanese transplantation donors.


Method: This cross sectional study was conduct in National Cancer Institute University of Gezira, Sudan. One hundred and forty six healthy Sudanese transplantation donors were enrolled in this study. Two ml of venous blood was collected from each participant and stored at -20 till DNA extraction. Qaigen kit was used for DNA extraction. VDR Fok1 was genotyped by CTPP-PCR with proper primers designing and PCR condition. Data was collected by structured questionnaire and analyzed by SNP Stats online tool and medical calculator.


Results: According to study results theFok1 polymorphism distributed among Sudanese with percentage of 49.9% (TT + CT), frequency of SNP variants was 69.8% regarding C allele and 30.2% for T allele, while the three genotypes was 10.2% for TT, 39.7% for TC and 50.1% for CC genotype.


Conclusion: The frequency of Fok1 VDR SNP was high among healthy Sudanese transplantation donors, this necessitate the conduction of wide area survey among other healthy Sudanese population. This intern will help in investigating the nature of the relation of this SNP with other different diseases.


Keywords: Vitamin D, Fok1rs. 2225870, VDR SNP, Sudanese

Keywords: Vitamin D, Fok1rs. 2225870, VDR SNP, Sudanese

Downloads

Download data is not yet available.

Author Biographies

Amira S. Khalafalla, Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Yousif A. Mohammed, National Cancer Institute, University of Gezira, Sudan

National Cancer Institute, University of Gezira, Sudan

Adam D. Abakar, Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

A Mergani, Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Amer M. Dafaalla, Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Mohammed Abdelwahed, Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan

Omer Balla, Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Mutaz I. Hassan, Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Shendi University, Sudan

Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Shendi University, Sudan

References

1. Al-Janabi, A. M., et al. "Association of Vitamin D Receptor-FOKI Gene Polymorphism with Breast Cancer Risk in Iraqi Female Patients."
2. Al-Shoumer, K. A. and T. M. Al-Essa "Is there a relationship between vitamin D with insulin resistance and diabetes mellitus?" World journal of diabetes 2015; 6(8):1057. https://doi.org/10.4239/wjd.v6.i8.1057
3. Al Safar, H., et al. "Vitamin D receptor gene polymorphisms among Emirati patients with type 2 diabetes mellitus." The Journal of steroid biochemistry and molecular biology 2018; 175:119-124. https://doi.org/10.1016/j.jsbmb.2017.03.012
4. Alkareem, A. and S. H. Hussien, Assessment of Serum 25 (OH) Vitamin D Concentration and Bsml Vitamin D Receptor Polymorphism in Sudanese Patients with Type 2 Diabetes Mellitus, Khartoum Teaching Hospital, Sudan,(2015–2016), University of Gezira. 2017
5. Beysel, S., et al. "VDR gene FokI polymorphism as a poor prognostic factor for papillary thyroid cancer." Tumor Biology 2018; 40(11):1010428318811766. https://doi.org/10.1177/1010428318811766
6. Bid, H. K., et al. "Vitamin-D receptor (VDR) gene (Fok-I, Taq-I and Apa-I) polymorphisms in healthy individuals from north Indian population." Asian Pac J Cancer Prev 2005; 6(2):147-152.
7. Cheng, W.-C. and K. Tsai, "The Vitamin D Receptor Start Codon Polymorphism (Fok 1) and Bone Mineral Density in Premenopausal Women in Taiwan." Osteoporosis international 1999; 9(6):545-549. https://doi.org/10.1007/s004390051102
8. Correa-Cerro, L., et al. "Vitamin D receptor polymorphisms as markers in prostate cancer." Human genetics 1999; 105(3):281-287.
9. El-Shorbagy, H. M., et al. "Association of vitamin D receptor gene polymorphisms with breast cancer risk in an Egyptian population." Tumor Biology 2017; 39(10):1010428317727738. https://doi.org/10.1177/1010428317727738
10. Gnagnarella, P., et al. Vitamin D Receptor Polymorphisms and Cancer. Sunlight, Vitamin D and Skin Cancer, Springer: 2020; 53-114.
11. Gross, C., et al. "The presence of a polymorphism at the translation initiation site of the vitamin D receptor gene is associated with low bone mineral density in postmenopausal Mexican‐American women." Journal of Bone and Mineral Research 1996; 11(12):1850-1855. https://doi.org/10.1002/jbmr.5650111204
12. HADDAD, S. "Vitamin-D receptor (VDR) gene polymorphism (FokI) in Syrian healthy population." Gene Reports 2021; 23:101083. https://doi.org/10.1016/j.genrep.2021.101083
13. Hama, T., et al. "Prognostic significance of vitamin D receptor polymorphisms in head and neck squamous cell carcinoma." PLoS One 2011; 6(12):e29634. https://doi.org/10.1371/journal.pone.0029634
14. Hitchon, C. A., et al. "Vitamin D receptor polymorphism rs2228570 (Fok1) is associated with rheumatoid arthritis in North American natives." The Journal of rheumatology 2012; 39(9):1792-1797. https://doi.org/10.3899/jrheum.120387
15. Hutchinson, P. E., et al. "Vitamin D receptor polymorphisms are associated with altered prognosis in patients with malignant melanoma." Clinical Cancer Research 2000; 6(2):498-504.
16. Ibrahim, Y. A., et al. "Association of Vitamin D Receptor (VDR) Start Codon FokI Polymorphism with Acute Myelogenous Leukemia." European Academic Research 2015; 2:15474-15482.
17. Iqbal, M. u. N. and T. A. Khan "Association between vitamin D receptor (Cdx2, Fok1, Bsm1, Apa1, Bgl1, Taq1, and Poly (A)) gene polymorphism and breast cancer: a systematic review and meta-analysis." Tumor Biology 2017; 39(10):1010428317731280. https://doi.org/10.1177/1010428317731280
18. Kaftan, A. N., et al. "Association of sunshine vitamin receptor gene polymorphisms (rs 2228570) and (rs7975232) with the type 2 diabetes mellitus in Iraqi patients from the middle Euphrates region." Gene Reports 2021; 22:100977. https://doi.org/10.1016/j.genrep.2020.100977
19. Karasneh, J. A., et al. "Association of vitamin D receptor gene polymorphisms with chronic and aggressive periodontitis in Jordanian patients." European journal of oral sciences 2013; 121(6):551-558. https://doi.org/10.1111/eos.12085
20. Khan, M. I., et al. "Vitamin D receptor gene polymorphisms in breast and renal cancer: current state and future approaches." International journal of oncology 2014; 44(2):349-363. https://doi.org/10.3892/ijo.2013.2204
21. Kotowicz, M., et al. "Vitamin D receptor start codon polymorphism is not associated with bone mineral density in Australian women." Bone 1998; 23:S372.
22. Liu, Y., et al. "Polymorphisms in the vitamin D receptor (VDR) and the risk of ovarian cancer: a meta-analysis." PLoS One 2013; 8(6):e66716. https://doi.org/10.1371/journal.pone.0066716
23. Minamitani, K., et al. "Difference in height associated with a translation start site polymorphism in the vitamin D receptor gene." Pediatric research 1998; 44(5):628-632. https://doi.org/10.1203/00006450-199811000-00002
24. Neela, V. S. K., et al. "Association of Taq I, Fok I and Apa I polymorphisms in Vitamin D Receptor (VDR) gene with leprosy." Human immunology 2015; 76(6):402-405. https://doi.org/10.1016/j.humimm.2015.04.002
25. Osman, E., et al. "Frequency of rs731236 (Taql), rs2228570 (Fok1) of Vitamin-D Receptor (VDR) gene in Emirati healthy population." Meta gene 2015; 6:49-52. https://doi.org/10.1016/j.mgene.2015.09.001
26. Rashid, M. U., et al. "Association between the BsmI Polymorphism in the Vitamin D receptor gene and breast cancer risk: Results from a Pakistani case-control study." PLoS One 2015; 10(10). https://doi.org/10.1371/journal.pone.0141562
27. Raza, S., et al. "Molecular mechanism of cancer susceptibility associated with Fok1 single nucleotide polymorphism of VDR in relation to breast cancer." Asian Pacific Journal of Cancer Prevention: APJCP, 2019; 20(1):199. https://doi.org/10.31557/APJCP.2019.20.1.199
28. Selvaraj, P., et al. "Association of vitamin D receptor gene variants of BsmI, ApaI and FokI polymorphisms with susceptibility or resistance to pulmonary tuberculosis." Current Science: 2003; 1564-1568.
29. Shahabi, A., et al. "Vitamin D receptor gene polymorphism: association with susceptibility to early-onset breast cancer in Iranian, BRCA1/2-mutation carrier and non-carrier patients." Pathology & Oncology Research 2018; 24(3): 601-607. https://doi.org/10.1007/s12253-017-0281-8
30. Sung, C.-C., et al. "Role of vitamin D in insulin resistance." Journal of biomedicine and biotechnology. 2012. https://doi.org/10.1155/2012/634195
31. Videman, T., et al. "Intragenic polymorphisms of the vitamin D receptor gene associated with intervertebral disc degeneration." Spine, 1998; 23(23):2477-2485. https://doi.org/10.1097/00007632-199812010-00002
32. Yousif, S. O., et al. Vitamin D receptor (VDR) gene polymorphisms among Sudanese females with breast cancer attending Radio and Isotope Center of Khartoum, AACR. 2017. https://doi.org/10.1158/1538-7445.AM2017-1455
33. Zmuda, J. M., et al. "Molecular epidemiology of vitamin D receptor gene variants." Epidemiologic reviews, 2000; 22(2):203-217. https://doi.org/10.1093/oxfordjournals.epirev.a018033
Statistics
57 Views | 3 Downloads
How to Cite
1.
Khalafalla AS, Mohammed YA, Abakar AD, Mergani A, Dafaalla AM, Abdelwahed M, Balla O, Hassan MI. Frequency and Distribution of Fok1rs 2228570 of Vitamin D Receptor Gene among Healthy Sudanese Population. JDDT [Internet]. 15Sep.2021 [cited 22Oct.2021];11(5):61-4. Available from: http://jddtonline.info/index.php/jddt/article/view/4990