Analysis of Achyranthes aspera L. extracts for their α-Amylase and urease inhibitory activities
Plant products have served as a major source of useful drugs for centuries and about half of the pharmaceuticals in use today are derived from these natural products. The efficacy and safety of herbal medicines have attracted the attention of scientists towards medicinal plants research. The present study was designed to investigate the whole plant extracts of Achyranthes aspera L. for their anti-enzyme (α-amylae and urease) activity. All the extracts of A. aspera were reported to show good alpha-amylase and urease inhibitory activity of greater than 50% in all the solvents used at a concentration of 1 mg/mL. α- amylase inhibition ranged from 17.66±0.10-69.90±0.50% whereas urease inhibitory activity ranged from 18.60±0.50 to 79.90±0.50% suggesting a strong α- amylase and urease inhibitory effects of this plant. Thus the study provided scientific evidence to the traditional uses of this plant in the treatment of obesity, diabetes, ulcers, kidney stones intestinal infections etc. Therefore, the whole plant extracts of A. aspera can be selected for further investigation to find their therapeutic potential.
Keywords: Achyranthes aspera, plant extracts, α-Amylase, Urease
2. Macegoniuk K, Inhibitors of bacterial and plants urease: A review, Folia Biologica et Oecologica, 2013; 9:9-16.
3. Azizian H, Nabati F, Sharifi A, Siavoshi F, Mahdayi M, Amanlou M, Large scale virtual screening for the identification of new Helicobacter pylori urease inhibitor scaffolds, Journal of Molecular Modeling, 2012; 18:2917-2927.
4. Prakash V, Rana S, Sagar A, Studies on analysis of antioxidant and enzyme inhibitory activity of Vitex negundo Linn., International Journal of Pharmacognosy and Phytochemical Research, 2017; 9:833-839.
5. Prakash V, Sagar A, Jhamta R, Kaundal P, Rana S, Kumari K, Rana J, To screen some medicinal plants of District Sirmaur, Himachal Pradesh for the presence of different phytochemicals in them, Plant Archives. 2019; 2321-2324.
6. Pavithra MKS, Kannan KM, In vitro enzyme inhibitory and cytotoxic studies with Evolvulus alsinoides (Linn.) Linn. Leaf extract: a plant from Ayurveda recognized as Dasapushpam for the management of Alzheimer’s disease and diabetes mellitus, BMC Complementary Medicine and Therapies, 2020; 20:1-12.
7. Anonymous, The Wealth of India, Raw Materials. New Delhi: Publication and Information Directorate, Center for Scientific and Industrial Research (CSIR), 1985; 57:168-170.
8. Selvanayagam ZE, Gnanavendan SG, Balakrishnan K, Rao RB, Anti-snake venom botanicals from ethnomedicine, Journal of Herbs, Spices and Medicinal Plants, 1994; 2:45-100.
9. Ratra PS, Misra K C, Seasonal variation in chemical composition of A. aspera and A. bidentata, Indian Forester, 1970; 96:372-375.
10. Singleton P, Bacteria in Biology, Biotechnology and Medicine. Fourth edition. New York, John Wiley and Sons Ltd. 1999.
11. Shukla R, Chakravarty M, Gautam MP, Indigenous medicine used for treatment of gynecological disorders by tribals of Chhattisgarh, India, Journal of Medicinal Plants Research, 2008; 2:356-360.
12. Giancarlo S, Rosa LM, Nadjafi F, Francesco M, Hypoglycaemic activity of two spices extracts: Rhus coriaria L. and Bunium persicum, Natural Products Research, 2006; 20:882-886.
13. Weatherburn MW, Phenol hypochlorite reaction for determination of ammonia, Analytical Chemistry, 1967; 39:971- 974.
14. Ashok KBS, Lakshman K, Nandeesh R, Kumar PA, Manoj B, Kumar V, In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats, Saudi Journal of Biological Sciences, 2011; 18:15.
15. Nyenwe EA, Jerkins TW, Umpierrez GE, Kitabchi AE, Management of type 2 diabetes: Evolving strategies for the treatment of patients with type 2 diabetes, Metabolism, 2011; 60:1-23.
16. Bonora E, Muggeo M, Post-prandial blood glucose as a risk factor for cardiovascular disease in type 2 diabetes: The epidemiological evidence, Diabetologia, 2001; 44:2107-2114.
17. Ahmed D, Ejaz N, Saeed R, Dar P, Cooking effect on anti-oxidative and alpha-amylase inhibitory potential of aqueous extract of Lagenaria siceraria fruit and its nutritional properties, Free Radicals and Antioxidants, 2016; 6:44-50.
18. Ciurli S, Benini S, Rypniewski WR, Wison KS, Miletti S, Mangani S, Structural properties of the nickel ions in urease: Novel insight into the catalytic and inhibition mechanisms. Coordination Chemistry Reviews, 1999; 190:331-335.
19. Ran N, Sharma SK, Vasudeva N, Assessment of anti-obesity potential of Achyranthes aspera Linn. Seed, Evidence-Based Complementary and Alternative Medicine, 2012; 1-7.
20. Ali I, Mabunni S, Mounica N, Kuldeep P, Kumar TJ, In vitro urease inhibitory activity of four selected medicinal plant extracts, International Journal of Pharma Research and Health Sciences, 2015; 3:891-894.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).