Anti-Inflammatory Activity of Extract Mixture of Annona senegalensis Pers. and Piliostigma thonningii (Schum.)

  • Komlatsè Togbenou Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé
  • Kokou Idoh Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé
  • Kossivi Dosseh Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé
  • Tchazou Kpatcha Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé
  • Amégnona Agbonon Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Abstract

Introduction: Annona senegalensis Pers (Annonaceae) and Piliostigma thonningii (Schum.) (Leguminoseae)  are two medicinal plants used, often in combination, in traditional Togolese medicine for the treatment of diseases with an inflammatory component.


Objective: The aim of this study is to evaluate the anti-inflammatory effect of the hydro-ethanolic extract (EHEM) of the combination of A. senegalensis and P. thonningii (1: 1, m: m).


Methods: The ovalbumin-induced allergic airway asthma model was used. Animals made asthmatic were treated with EHEM at doses of 250 and 500 mg / kg. Inflammation markers including histamine, nitric oxide (NO), vascular leakage, leukocyte infiltration in the airways, and malondialdehyde (MDA), were measured.


Results: Compared to the SNT group, EHEM inhibits the infiltration of the airways by leukocytes (850,00 × 103 ± 50 cells / mL vs 1830 × 103 ± 53,85 cells / mL for ST500 mg / kg, P <0,05). It inhibited vascular permeability to Evans Blue (10,40 ± 0,270 μg / mL vs ST500, p<0,05). It inhibited histamine release (13,95 ± 0,937 μg / mL vs 32,78 ± 1,044 μg / mL, p<0,05) and NO production (0,211 ± 0,008 Μm vs 0,315 ± 0,022 μM, p<0,05). It finally inhibited MDA production (14,66 ± 0,533 nM / mL vs 9,014 ± 0,366 nM / mL and 7,149 ± 0,300 nM / mL, p<0,05) in lung tissue.


Conclusion: Our results suggest that EHEM inhibits OVA-induced inflammation. These results justify the use of this combination of plants in traditional Togolese medicine.


Keywords: Inflammation, malondialdehyde, Annona senegalensis, Piliostigma thonningii.

Keywords: Inflammation, malondialdehyde, Annona senegalensis, Piliostigma thonningii

Downloads

Download data is not yet available.

Author Biographies

Komlatsè Togbenou, Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Kokou Idoh, Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Kossivi Dosseh, Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Tchazou Kpatcha, Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Amégnona Agbonon, Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé

References

[1] Komakech R, Kim Y, Matsabisa GM, Kang Y. Anti-inflammatory and Analgesic Potential of Tamarindus indica Linn. (Fabaceae): A Narrative Review. Integrative Medicine Research. 1 sept 2019; 8(3):181‑6.
[2] Steven S, Frenis K, Oelze M, Kalinovic S, Kuntic M, Bayo Jimenez MT, et al. Vascular Inflammation and Oxidative Stress: Major Triggers for Cardiovascular Disease. Oxid Med Cell Longev [Internet]. 23 juin 2019 [Cited on October 20, 2020];2019. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612399/
[3] Holt S, Masoli M, Beasley R. The use of the self-management plan system of care in adult asthma. Prim Care Respir J. mars 2004;13(1):19‑27.
[4] Regional Committee for Africa 50. Promoting the role of traditional medicine in health systems: A strategy for the African Region. 2000 [Cited on October 20, 2020]; Available from: https://apps.who.int/iris/handle/10665/95467
[5] Mamedov N, Craker L. Medicinal Plants Used for the Treatment of Bronchial Asthma in Russia and Central Asia. Journal of Herbs, Spices & Medicinal Plants. 11 juill 2001; 8:91‑117.
[6] Bhat SS, Hegde KS, Chandrashekhar S, Rao SN, Manikkoth S. Preclinical screening of Phyllanthus Amarus Ethanolic Extract for its Analgesic and Antimicrobial Activity. Pharmacognosy Res. 2015; 7(4):378‑84.
[7] Okhale SE, Akpan E, Fatokun OT, Esievo KB, Kunle OF. Annona senegalensis Persoon (Annonaceae): A review of its Ethnomedicinal Uses, Biological Activities and Phytocompounds. Journal of Pharmacognosy and Phytochemistry. 2016;5(2):211.
[8] Okoli CO, Onyeto CA, Akpa BP, Ezike AC, Akah PA, Okoye TC. Neuropharmacological Evaluation of Annona senegalensis leaves. African Journal of Biotechnology. 2010; 9(49):8435–8444.
[9] Okoye TC, Akah PA, Omeje EO, Okoye FB, Nworu CS. Anticonvulsant effect of kaurenoic acid isolated from the root bark of Annona senegalensis. Pharmacology Biochemistry and Behavior. 2013; 109:38–43.
[10] Lame Y, Nukenine EN, Pierre DYS, Elijah AE, Esimone CO. Laboratory Evaluations of the Fractions Efficacy of Annona senegalensis (Annonaceae) Leaf Extract on Immature Stage Development of Malarial and Filarial Mosquito Vectors. Journal of arthropod-borne diseases. 2015; 9(2):226.
[11] Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree Database: A tree Reference and Selection Guide Version 4.0. 2009. Url: http://www worldagroforestry org/af/treedb/(Accessed on 15 February, 2011). 2015.
[12] Rist G. Le développement-4 édition: Histoire d’une Croyance Occidentale. 4 édition revue et Augmentée. Presses de Sciences Po; 2015.
[13] Kerharo J, Adam J-G. La Pharmacopée sénégalaise Traditionnelle: Plantes Médicinales et Toxiques. 1974;
[14] DIABATE MDM. Etude de la Phytochimie et des Activités Biologiques d’une Recette Traditionnelle Utilisée dans le Traitement de l’hypertrophie Bénigne de la Prostate au Mali. 2006;
[15] Agbonon A, Aklikokou K, Eklu-Gadegbeku K, Akpagana K, Gbeassor M. Effect of Ethanolic Extract of Pluchea ovalis root in Asthma Model of Wistar Rat. Boletin Latinoamericano y del caribe de plantas medicinales y Aromaticas. 2005; 4(3):52–59.
[16] Lilly CM, Chapman RW, Sehring SJ, Mauser PJ, Egan RW, Drazen JM. Effects of Interleukin 5-induced Pulmonary Eosinophilia on Airway Reactivity in the guinea pig. American Journal of Physiology-Lung Cellular and Molecular Physiology. 1996; 270(3):L368–L375.
[17] Yang XX, Powell WS, Hojo M, Martin JG. Hyperpnea-induced Bronchoconstriction is Dependent on Tachykinin-induced Cysteinyl Leukotriene Synthesis. Journal of Applied Physiology. 1997; 82(2):538–544.
[18] Agbonon A, Gbeassor M. Hepatoprotective Effect of Lonchocarpus sericeus leaves in CCl4-induced Liver Damage. Journal of herbs, spices & medicinal plants. 2009; 15(2):216–226.
[19] Dosseh K, Kpatcha T, Adjrah Y, Idoh K, Agbonon A, Gbéassor M. Antiinflammatory Effects of Byrsocarpus coccineus shum and thonn (Connaraceae) root. World J Pharmaceut Res. 2014;3:3585–3598.
[20] Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Banes AJ, Guilak F. The Effects of Static and Intermittent Compression on Nitric Oxide Production in Articular Cartilage Explants. Journal of Orthopaedic Research. 2001; 19(4):729–737.
[21] Roh S-S, Kim S-H, Lee Y-C, Seo Y-B. Effects of radix adenophorae and cyclosporine A on an OVA-induced murine model of asthma by suppressing to T cells activity, eosinophilia, and bronchial hyperresponsiveness. Mediators of inflammation. 2008; 2008.
[22] Suralkar AA, Kasture SB. Inhibitory Effect of Abrus Precatorious Extract on Bronchial Hyper-reactivity Induced by Ovalbumin in Experimental Animals. Int J Pharm Pharm Sci. 2013; 5(1):403–404.
[23] Schuster M, Tschernig T, Krug N, Pabst R. Lymphocytes Migrate from the Blood into the Bronchoalveolar Lavage and Lung Parenchyma in the Asthma Model of the Brown Norway rat. American Journal of Respiratory And Critical Care Medicine. 2000; 161(2):558–566.
[24] Awortwe C, Sackeyfio AC, Osei-Safo D, Bugyei KA, Asiedu-Gyekye IJ. Dual Effect of Taraxacum officinale Leaves: Anticholinergic and Inhibitory Effect on Inflammatory cells in Ovalbumin-sensitized guinea-pigs. African Journal of Pharmacy and Pharmacology. 2011; 5(23):2613–2619.
[25] Lindner I, Meier C, Url A, Unger H, Grassauer A, Prieschl-Grassauer E, et al. Beta-escin has Potent Anti-allergic Efficacy and Reduces Allergic Airway Inflammation. BMC immunology. 2010; 11(1):24.
[26] Holgate ST. Pathogenesis of asthma. Clinical & Experimental Allergy. 2008; 38(6):872–897.
[27] Cochet M, Pannetier C, Regnault A, Darche S, Leclerc C, Kourilsky P. Molecular Detection and in vivo Analysis of the Specific T cell Response to a Protein Antigen. European Journal of Immunology. 1992; 22(10):2639–2647.
[28] Kim DY, Yang WM. Panax ginseng Ameliorates Airway Inflammation in an Ovalbumin-sensitized Mouse Allergic Asthma Model. Journal of Ethnopharmacology. 2011; 136(1):230–235.
[29] Egharevba HO, Kunle FO. Preliminary Phytochemical and Proximate Analysis of the Leaves of Piliostigma thonningii (Schumach.) Milne-Redhead. Ethnobotanical Leaflets. 2010; 2010(5):2.
[30] Johnson TO, Olatunde A, Nwachukwu L. Phytochemical Composition of Annona senegalensis Leaf and its Antioxidant Activity during Trypanosoma brucei brucei induced Oxidative Stress in Mice. Journal of Pharmacy & Bioresources. 2017; 14(2):219–227.
[31] Powers SK, Smuder AJ, Kavazis AN, Hudson MB. Experimental Guidelines for Studies Designed to Investigate the Impact of Antioxidant Supplementation on Exercise Performance. International Journal of Sport Nutrition and Exercise Metabolism. 2010; 20(1):2–14.
[32] Bloomer RJ, Fisher-Wellman KH. Blood oxidative Stress Biomarkers: Influence of Sex, Exercise Training Status, and Dietary intake. Gender Medicine. 2008; 5(3):218–228.
[33] Jung H-J, Nam JH, Choi J, Lee K-T, Park H-J. Antiinflammatory Effects of Chiisanoside and Chiisanogenin Obtained from the Leaves of Acanthopanax Chiisanensis in the Carrageenan-and Freund’s complete Adjuvant-induced rats. Journal of Ethnopharmacology. 2005; 97(2):359–367.
[34] Terziev L, Dancheva V, Shopova V, Stavreva G. Antioxidant Effect of MnTE-2-PyP on lung in Asthma Mice Model. The Scientific World Journal. 2012; 2012.
[35] Al-Afaleg NO, Al-Senaidy A, El-Ansary A. Oxidative Stress and Antioxidant Status in Saudi Asthmatic Patients. Clinical Biochemistry. 2011; 44(8‑9):612–617.
[36] Ladhar-Chaabouni R, Gargouri R, Hamza Chaffai A. Effect of Cadmium on Some Biomarkers in the Clam Ruditapes Decussatus: Metallothionein Quantification Using two Techniques. International Journal of Environment and Pollution. 2007;30(3‑4):593–605.
[37] Giguère A, Couillard Y, Campbell PG, Perceval O, Hare L, Pinel-Alloul B, et al. Steady-state Distribution of Metals among Metallothionein and Other Cytosolic Ligands and Links to Cytotoxicity in Bivalves living along a Polymetallic Gradient. Aquatic Toxicology. 2003; 64(2):185–200.
[38] Missebukpo A, Metowogo K, Diallo A, Lawson-Evi P, Eklu-Gadegbeku K, Aklikokou KA, et al. Antioxidant Effects of Lxora coccinea Linn. in a Rat Model of Ovalbumin-induced Asthma. African Journal of Pharmacy and Pharmacology. 2013; 7(42):2794–2800.
[39] Zhu H, Li YR. Oxidative Stress and Redox Signaling Mechanisms of Inflammatory Bowel Disease: Updated Experimental and Clinical Evidence. Experimental Biology and Medicine. 2012; 237(5):474–480.
[40] Napolitano DR, Mineo JR, De Souza MA, De Paula JE, Espindola LS, Espindola FS. Down-modulation of Nitric Oxide Production in Murine Macrophages Treated with Crude Plant Extracts from the Brazilian Cerrado. Journal of Ethnopharmacology. 2005; 99(1):37–41.
[41] Laskin DL, Fakhrzadeh L, Laskin JD. Nitric Oxide and Peroxynitrite in Ozone-induced Lung Injury. In: Biological Reactive Intermediates VI. Springer; 2001. p. 183–190.
[42] Van der Vliet A, Eiserich JP, Cross CE. Nitric oxide: A Pro-inflammatory Mediator in Lung Disease? Respiratory Research. 2000; 1(2):1.
[43] Shin I-S, Lee M-Y, Ha H, Jeon W-Y, Seo C-S, Shin H-K. Dianthus Superbus Fructus Suppresses Airway Inflammation by Downregulating of Inducible Nitric Oxide Synthase in an Ovalbumin-induced Murine Model of Asthma. J Inflamm (Lond). 30 oct 2012; 9(1):41.
Statistics
64 Views | 52 Downloads
How to Cite
1.
Togbenou K, Idoh K, Dosseh K, Kpatcha T, Agbonon A. Anti-Inflammatory Activity of Extract Mixture of Annona senegalensis Pers. and Piliostigma thonningii (Schum.). JDDT [Internet]. 20Dec.2020 [cited 21Jan.2021];10(6-s):103-8. Available from: http://jddtonline.info/index.php/jddt/article/view/4605