Nephroprotective Effect of Asgand Powder (Withania somnifera Dunal) on Cisplatin Induced Renal Injury in Rats
Background: The nephroprotective effect of crude powder of Asgand (Withania samnifera Dunal) was studied against cisplatin induced renal toxicity in wistar albino rats of either sex.
Results: The powder of Withania somnifera Dunal at dose level 700 and 1400 mg/kg body wt/day showed reduction in elevated blood urea, serum creatinine and uric acid. It was found to protect kidney damage by cisplatin induced nephrotoxicity as evidenced by oral administration of Asgand (Withania samnifera Dunal) (700 mg/kg) inhibited the rise in blood urea nitrogen (121.7%), Serum cratinine (76.64%), and uric acid (92.7%). There were 92.71% inhibition in the rise of BUN, 92% inhibition in the rise of serum creatinine and 106.6% inhibition in the rise of uric acid with 1400 mg.
Conclusion: The findings suggest that the famous Unani herb Asgand Powder possesses marked nephroprotective activity and could offer a promising role in the treatment of acute renal injury caused by nephrotoxins like cisplatin.
Keywords: Withania somnifera Dunal, Cisplatin, Asgand, Nephroprotection, Unani Medicine
2. Livingston RB. Cisplatin in the treatment of solid tumors: effect of dose and schedule. J. National Cancer Institute, 1989; 81(10):724-725.
3. Kuhlmann MK, Burkhardt G, Kohler H, Insights into potential cellular mechanisms of cisplatin nephrotoxicity and their clinical application, Nephrol Dial. Tranplant, 1997; 12(12):2478-2480.
4. Garnick MB, Mayer RJ, Abelson HT. Acute renal failure associated with cancer treatment. In: Acute renal failure. Eds. B.M. Brenner & J.M. Lazarus. Churchill Livingstone, New York; 1988. P. 621-657.
5. Madias NE, Harrington JT, Platinum nephotoxicty, American Journal of Medicine, 1978; 65(1):307-314.
6. Goldstein RS, Mayor GH. The nephrotoxicity of cisplatin. Life Sciences, 1983; 32:685-690.
7. Zhang J, Lindup E, Cisplatin nephrotoxicity: decreases in mitochondrial protein sulphydryl concentration and calcium uptake by mitochondria from rat renal cortical slices, Biochem, Pharmacol, 1994; 47 (7):1127-1135.
8. Misitry P, Merazga Y, Spargo DJ, Riley PA, Mc Brien DCH, The effects of cisplatin on the concntration of protein thiols and glutathione in the rat kidney, Cancer Chemother. Pharmacol., 1991; 28(4):277-282.
9. Inselmann G, Blohmer A, Kottny W, Nellessen U, Heidemann HT, Modification of cisplatin-induced renal p aminohippurate uptake alteration and lipid peroxidation by thiols, Ginkgo biloba extract, desferroxamine and torbafyline, Nephron, 1995; 70(4):425-429.
10. Kandaswami C, Middleton Jr. E, Free Radical Scavenging and Antioxidant Activity of Plant Flavonoids, Free Radicals in Diagnostic Medicine, 1994; 366:351-376.
11. Sadzuka Y, Shoji T, Takino Y, Effect of cisplatin on the activities of enzymes which protect against lipid per oxidation, Biochem. Pharmacol., 1992; 43(8):1872-1875.
12. Offerman JJC, Meijer S, Sleijfer DT, Mulder NH, Donker AJM, Koops HS, Vander Hem GK, Acute effects of cis-diamminedichloroplatinum (CDDP) on renal function, Cancer Chemother, Pharmacol., 1984; 12(1):36-38.
13. Li Q, Bowmer CJ, Yates MS, Effect of arginine on cisplatin-induced acute, renal failure in the rat, Biochem. Pharmacol., 1994; 47(12):2298-2301.
14. Nakano S. Gemba M, Potentiation of cisplatin-iduced lipid peroxidation in kidney cortical slices by glutathione depletion, Jpn J Pharmacol., 1989; 50(2):87-92.
15. Zhong LF, Zhang JG, Zhang M, Xia YX, Protection against cisplatin induced lipid peroxidation and kidney damage by procaine in rats, Arch Toxicol., 1990; 64(7):599-600.
16. Baldew GS, Van den Hamer CJA, Los G, Vermuelen NPE, de Goeji JJM, McVie JG, Selnium induced protection against cis-diaminodichloroplatinum nephrotoxicty in mice and rats, Cancer Research., 1989; 49(11):3020-3023.
17. Anonymous, “Unani system of Medicine, The Science of health and healing”, Department of AYUSH, Ministry of Health and family welfare publication, New Delhi, 2013.
18. Ghani N. Khazainul Advia. Vol-II, Barqi press Lahore. 1926. P. 79-81.
19. Khan MA. Muheet-e-Azam. (urdu translation), vol-I, Central council for Research in Unani Medicine Publication, New Delhi, 2012. P. 319-320.
20. Azhar MU, Alam M, Aslam M, Javed K, Jarfi MA, Role of Asgand (Withania somnifera Dunal) in cadmium chloride induced nephrotoxicity in rats, Hamdard Medicus, 2005; 48(3):48-51.
21. Alam MMA, Javed K, Jafri MA. Effect of revand (Rheum emodi) on renal functions in rats. Journal of Ethnopharmacology, 2005; 96(1-2):121-125.
22. Azhar MU, Khan SA, Aslam M, Javed K, Jarfi MA, Nephroprotective activity of kulthi (Dolichos biflorous) on gentamicin induced toxicity in rats, Journal Science and Pharmacy, 2004; 5(2):50-53.
23. Azhar MU, Tajuddin, Jafri MA, Nephroprotective effect of kulthi [Macrotyloma uniflorum (lam) verdc.] on acute renal failure in rats, Hippocratic Journal of Unani Medicine, 2008; 3(4):97-102.
24. Alam M, Javed K, Jafri MA, Effect of oleo-gum-resin of Boswellia serrata (Kundur) on renal functions in Albino rats, Indian Journal of Traditional Knowledge, 2011; 10(4):736-740.
25. Akhtar F, Azhar MU, Aslam M, Javed K, Nephroprotective effect of Khar-e-Khasak Khurd (Tribulus terristris linn) on gentamicin-induced experimental nephrotoxicity in rats, Asian Journal of Research in Nephrology, 2020; 3(3):6-13.
26. Javaid R, Aslam M, Javed R, Nizami Q, Javaid K, Azhar MU, Extract of ferula foetida Regal reverses gentamicin induced nephrotoxicity in rats, Experimental and Clinical Sciences Journal, 2012; 11-1-7.
27. Rao M, Rao MNA, Protective Effect of Cystone a polyherbal Ayurvedic preparation on cisplatin induced renal toxicity in rats, Journal of Ethnopharmacology, 1998; 62(1):1-6.
28. Godkar PB. Text Book of Medical Laboratory Technology. Bhalani Publishing House, Bombay, 1994. P. 174-185.
29. Devipriya S, Shyamaladevim CS, Protective effect of quercetin in cisplatin induced cell injury in the rat kidney, Indian J. Pharmacol., 1999; 31(6):422-426.
30. Azhar MU, Anjum N, Quddusi N, Akhtar J, Akram U, Yadav PK, Pharmacologically active nephroprotective plants- a review, Hamdrad Medicus, 2013; 56:56-76.
31. Azhar MU, Javed K, Jarfi MA, Plant with nephroprotective activity, Hamdard Medicus, 2005; 48(4):33-43.
32. Azhar MU, Akhtar F, Aslam M, Anwer M, Tajuddin, Jafri MA, Nephroprotective activity of some herbal preparations, Hamdard Medicus, 2005; 49(1):110-115.
33. Azhar MU, Effect of herbal unani formulation on nephrotic syndrome: a case study, Indian Journal of Traditional Knowledge, 2018; 17(4):807-810.
34. Azhar MU, Mustehasan, Alam M, Ahmad SG, Anjum N, Quddusi N, Nephroprotective unani drug Khar-e-Khasak Khurd (Tribulus terrestris linn.) - a review, International Journal of Scientific Research in Biological Sciences, 2020; 7(1):24-36.
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