In vitro antioxidant activity and gastroprotective effect of ethanolic extract from Cucumis melo L. var. inodorus fruit on ethanol-induced gastric ulcer in rats

  • Amel Bouaziz Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria
  • Assia Bentahar Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria
  • Saliha Djidel Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria
  • Saliha Dahamna Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria
  • Seddik Khennouf Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Abstract

The aim of this study was to estimate the content of polyphenols, flavonoids and tannins and to evaluate the antioxidant property and the antiulcer activity of the ethanolic extract of melon (Cucumis melo L. var. inodorus) pulp on ethanol-induced gastric ulcers in rats.  Ferric reducing power and hydroxyl radical scavenging tests were applied to evaluate the in vitro antioxidant activity. The polyphenolic and flavonoids contents of    melon extract were found to be 56.5 ± 2.49 mg GAE/g of dry extract and 0.43 ± 0.09 mg QE/ g of dry extract, respectively. Whereas the tannins content was 48. 30 ±  0. 9 mg ETA/ g of dry extract.  Melon pulp  extract exhibited  a good reducing potential   with  an  EC50 of  4.23 ± 0.08 mg/ml and high hydroxyl radical scavenging activity with IC50 of  1.83± 0.09 mg/ml. Oral administration of the melon pulp extract at doses of  200 and 600 mg/kg to rats  reduced gastric mucosal lesions  dose-dependently  with percentage protection of 56.56 and 93.79% compared to omeprazole (95.92 %) as reference drug. These results showed that the melon pulp extract had a good antioxidant activity and gastroprotective potential suggesting it use as an adjuvant in the treatment of gastric ulcer.


Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Ulcer, Rat.

Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Ulcer, Rat

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Author Biographies

Amel Bouaziz, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Assia Bentahar, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Saliha Djidel, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Saliha Dahamna, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Seddik Khennouf, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria

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Bouaziz A, Bentahar A, Djidel S, Dahamna S, Khennouf S. In vitro antioxidant activity and gastroprotective effect of ethanolic extract from Cucumis melo L. var. inodorus fruit on ethanol-induced gastric ulcer in rats. JDDT [Internet]. 29Oct.2020 [cited 26Nov.2021];10(5-s):302-7. Available from: http://jddtonline.info/index.php/jddt/article/view/4529