Isolation and Screening of Fungal Culture Isolated From Algerian Soil for the Production of Cellulase and Xylanase
Lignocellulolytic enzymes constitute a very large group of extracellular proteins secreting by fungi who is ecologically involved in the degradation of a variety of complex materials, a property that is attributed to a battery of enzymes produced by these microorganisms like cellulases and xylanases who are of significant industrial value and relevance. Forty fungal isolated from rich soil in organic matter were screened for lignocellulolytic enzymes production, its organized on the basis of their hydrolytic potential of cellulose and xylan. The isolates strains presented enzymatic activity which was ranked as follows: cellulolytic (56%), xylanolytic (44%). Some selected strains that produce high levels of enzymes (cellulase, xylanase) grown in submerged fermentation (SmF) and were quantitatively evaluated. The fermentation experiments were carried out in shake flasks. The highest CMCase (5,10 IU/ml) and xylanase (98,25 IU/ml) activities were obtained from Trichoderma sp strain Mtr6 isolate.
Keywords: Fungi, Trichoderma sp, lignocellulolytic enzymes, soil, screening, organic matter.
2. Dashtban M, Schraft H, Qin W, « Fungal bioconversion of lignocellulosic residues; Opportunities & perspectives» International Journal of Biological Sciences, 2009, 5:578–595.
3. Cunha L, Martarello R, de Souza PM, et al, « Optimization of Xylanase Production from Apergillus foetidus in Soybean Residue » Enzyme Research, 2018, 17:1–7.
4. Plempel M, Bremm, kD, Gao, Z, « Pathogenese –Faktoren von Dermatophyten» German Patent Aplication, 1991.
5. Vinod KN, Mary ER, Gunaseeli R, et al, « Process optimization and production kinetics for cellulase production by Trichoderma viride VKF3» Springer Plus, 2014; 3(1):92.
6. Tasia W, Melliawati R, « Cellulase and xylanase production from three isolates of indigenous endophytic fugi » Earth and Enviromental Science, 2017; 1-5.
7. Demain, AL, Adrio, JL, « Contributions of microorganisms to industrial biology » Molecular Biotechnology, 2008; 38:41–55.
8. Polizeli M, Rizzatti A, Monti R, et al, « Xylanase from fungi: Properties and industrial application» Applied Microbiology and biotechnology, 2005; 67(5):577-591.
9. Bhat MK, « Cellulases and related enzymes in biotechnology » Biotechnol Adv, 2000; 18(5):355–383.
10. Kuhad RC, Gupta R, Singh A « Microbial cellulases and their industrial applications» Enzyme Research, 2011; 20:11-24.
11. Subramaniyan S, Prema « Biotechnology of microbial xylanases: Enzymologie, molecular biology,and application » Critical Reviews in Biotechnology, 2002; 22(1):33-64.
12. Limayem A, Rike SC « Lignocellulosic biomass for bioethanol production: Curent perspectives, potential issues and future prospects » Progress in Energy and combustion science, 2012; 38(4):449-467.
13. Sankareswaran M, Kalaiselvi K, Anbalagan S, et al, « Production, cahracterisation and immobilized dye decolorization of amylase enzyme produced by Bacillus megabacterium isolated from soil sample » International Journal of advanced Research, 2015; 3:295-305.
14. Choi Y, Hodgkiss JJ, Hyde D, « Enzyme production by endophytes of Brucea javanica » Journal of Agriculture Science and Technology, 2005; 1:55–66.
15. Jahangeer S, Khan N, jahangeer S, et al, « Screening and characterization of fungal cellulase isolated from the native environmental source » Pakistan Journal of Botany, 2005; 37(3), 739-748.
16. Maria GL, Sridhar KR and Raviraja NS, « Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India » Journal of Agricultural Science and Technology, 2005; 1: 67-80.
17. Taeochapoempol K, Sreethawong T, Rangsunvigit P, et al, « Cellulase-Producing Bacteria from Thai Higher Termites, Microcerotermes sp.: Enzymatic Activities and Ionic Liquid Tolerance» Applied biochemistry and biotechnology, 2011; 164(2):204-219.
18. Gautam SP, Bundela PS, Pandey AK, et al, « Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi » Biotechnology Research International, 2011; 1: 1-8.
19. Wongpisal P, Aundre KN, Seephueak P, et al, « Effect of fungi for decomposition rate and nutrient content in oil palm leaf litter (Elaeis guineensis Jacq.) Songklanakarin» Journal of Plant Science, 2016; 3:9-14.
20. Naveen Kumar KJ, Thippeswamy B, « Isolation and screening of potential cellulolytic fungi from Areca nut husk waste» International Journal of Current Science, 2013; 8 :125-132.
21. Mandels M, Weber J, « The production of cellulases. In Cellulases and their Applications » Advances in Chemistry Series, (ed. R. F. Gould), Washington, DC: American Chemical Society, 1969; 95:391-414.
22. Pointing SB, « Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi » Fungal Diversity, 1999; 2:17- 33.
23. Philippoussis A, Diamantopoulou P, Papadopoulou K, et al, « Biomass, laccase and endoglucanase production by Lentinula edodes during solid state fermentation of reed grass, bean stalks and wheat straw residues » World Journal of Microbiology and Biotechnology, 2011; 27(2):285-297.
24. Chakraborty S, Gupta R, Jain KK, et al, « Cost-effective production of cellulose hydrolysing enzymes from Trichoderma sp. RCK65 under SSF and its evaluation in saccharification of cellulosic substrates » Bioprocess and Biosystems Engineering, 2016; 39: 1659-1670.
25. Ghose TK, « Measurement of cellulase activities» Pure and Applied Chemistry, 1987; 59:257–268.
26. Miller GL, «. Use of dinitrosalicyclic acid reagent of determination of reducing sugar. Analytical Chemistry, 1959; 31:426–429.
27. Favaro L, Jooste T, Basaglia M, et al, « Designing industrial yeasts for the consolidated bioprocessing of starchy biomass to ethanol » Bioengineered, 2013; 4(2):97-102.
28. Flannigan B, « Degradation of arabinoxylan and carboxymethyl cellulose by fungi isolated from barley kernels » Transactions of the British Mycological Society, 1970; 55:277-281.
29. Kasana RC, Salwan, R, Dhar H, et al, « A Rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine» Current Microbiology, 2008; 57:503–507.
30. Bano A, Chen X, Prasongsuk S, et al, « Purification and characterization of cellulase from obligate halophilic Aspergillus flavus (TISTR 3637) and its prospects for bioethanol production» Appl Biochem Biotechnol, 2019; 189:1327–1337.
31. Girio FM, Fonseca C, Carvalheiro F, et al, « Hemicelluloses for fuel ethanol: A review. Bioresour Technol, 2010; 101(13):4775–4800.
32. Selvam K, Govarthanan M, Kamala-Kannan S, et al « Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC» RSC Advanes, 2014; 4:13045-13051.
33. Kandasamy S, Muthusamy G, Balakrishna S, et al, « Optimization of protease production from surface-modified coffee pulp waste and corncobs using Bacillus sp. by SSF» 3 Biotech, 2016; 6(2):167.
34. Azzouz Z, Bettache A, Boucherba N, et al, « Optimization of xylanase production by newly isolated strain trichoderma afroharzianum isolate az 12 in solid state. fermentation using response surface methodology » Cellulose chemistry and technology, 2020; 54 (5-6):451-462.
35. Chandra M, Kalra A, Sharma PK, et al, « Optimization of cellulases production by Trichoderma citrinoviride on marc of Artemisia annua and its application for bioconversion process » Biomass and Bioenergy, 2010, 34:805–811.
36. Wong KKY, Saddler JN, Trichoderma xylanases, their properties and application, Crit Rev Biotechnol, 1992; 12:413-435.
37. Peterson R, Nevalainen H, « Trichoderma reesei RUT-C30- Thirty years of strain improvement» Microbiology, 2012; 158: 58-68.
38. Silveira FQP, Sousa MV, Ricart CAO, et al, new xylanase from a Trichoderma harzianum strain, J Ind Microbiol Biotechnol, 1999; 23:682-685.
39. Chen LL, Zhang M, Zhang DH, et al, « Purification and enzymatic characterization of two β-endoxylanases from Trichoderma sp. K9301 and their actions in xylooligosaccharide production » Bioresour Technol, 2009; 100:5230-5236.
40. Rajaa C, Franck D, Christelle D, et al, « Isolation, Identification and enzymatic activity of halotolerant and halophilic fungi from the great Sebkha of Oran in Northwestern of Algeria. Mycobiology, 2019; 47(2):230-241.
41. Webster J, Weber RWS, « Introduction to fungi, Cambridge University Press, 3rd ed: 2007; 875.
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