Isolation and Screening of Fungal Culture Isolated From Algerian Soil for the Production of Cellulase and Xylanase

  • Ouahiba GAHFIF Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria
  • Yasmina SOUAGUI Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria
  • Zahra AZZOUZ Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria
  • Sadrati NOUARI Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria
  • Zahir AMGHAR Zahir AMGHAR Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria
  • Nawel BOUCHERBA Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria
  • Mouloud KECHA Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria
  • Said BENALLAOUA Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria
  • Azzeddine Bettache Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Abstract

Lignocellulolytic enzymes constitute a very large group of extracellular proteins secreting by fungi who is ecologically involved in the degradation of a variety of complex materials, a property that is attributed to a battery of enzymes produced by these microorganisms like cellulases and xylanases who are of significant industrial value and relevance. Forty fungal isolated from rich soil in organic matter were screened for lignocellulolytic enzymes production, its organized on the basis of their hydrolytic potential of cellulose and xylan. The isolates strains presented enzymatic activity which was ranked as follows: cellulolytic (56%), xylanolytic (44%). Some selected strains that produce high levels of enzymes (cellulase, xylanase) grown in submerged fermentation (SmF) and were quantitatively evaluated. The fermentation experiments were carried out in shake flasks. The highest CMCase (5,10 IU/ml) and xylanase (98,25 IU/ml) activities were obtained from Trichoderma sp strain Mtr6 isolate.


Keywords: Fungi, Trichoderma sp, lignocellulolytic enzymes, soil, screening, organic matter.

Keywords: Fungi, Trichoderma sp, lignocellulolytic enzymes, soil, screening, organic matter

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Author Biographies

Ouahiba GAHFIF, Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Yasmina SOUAGUI, Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Zahra AZZOUZ, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Sadrati NOUARI, Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Laboratory of Characterization, Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj, Algeria

Zahir AMGHAR Zahir AMGHAR, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Nawel BOUCHERBA, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Mouloud KECHA, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Said BENALLAOUA, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Azzeddine Bettache, Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

Laboratoire de Microbiologie Appliquée, faculté des Sciences de la Nature et de la Vie, université de Bejaia, 06000 Bejaia, Algeria

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GAHFIF O, SOUAGUI Y, AZZOUZ Z, NOUARI S, Zahir AMGHAR ZA, BOUCHERBA N, KECHA M, BENALLAOUA S, Bettache A. Isolation and Screening of Fungal Culture Isolated From Algerian Soil for the Production of Cellulase and Xylanase. JDDT [Internet]. 15Oct.2020 [cited 21Oct.2020];10(5-s):108-13. Available from: http://jddtonline.info/index.php/jddt/article/view/4493