The Hepatoprotector Effect of Uncaria gambir Roxb Extract in Wistar Rats Induced by Paracetamol

  • , Suparni Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia
  • , Musthari Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia
  • Liza Mutia Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia
  • Mangoloi Sinurat Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia
  • Siti Syarifah Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia
  • Dila Sedana Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia
  • Dwi Rita Anggraini Department of Anatomy, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia
  • Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Abstract

Background: Drug induced liver injury (DILI) is known as the damage of liver cells due to chronic administrations of drug. The chronic administration of paracetamol could be trigger the damage of liver cells.The hepatoprotector agents are still limited worldwide.  Gambier(Uncaria gambir Roxb) is an Indonesia’traditional medicine which have many benefits as antioxidant, antiseptic, antidiarrhoea, etc  that commonly used in society. 


Method: The present study was conducted to investigate the hepatoprotector effect ofgambier in wistar rats induced by paracetamol.  The wistar rats were divided into seven groups and received the treatment orally for 12 days. Group I (aquadest), II(curcuma,400 mg/kgBW),III (gambier,26 mg/200gr), IV(gambier, 53 mg/200gr), V(gambier,106 mg/200gr),VI(gambier,212 mg/200gr) and VII(gambier,424mg/200gr). Termination, blood and liver organ collection were done after all group induced by paracetamol for two days. Histopatology changes of liver were examined using Hematoxycilline (HE) staining. AST and ALT levels were analyzed.


Results: There were significant differentiation of AST levels among the groups, especially between group I and group IV and between group II and group IV. The ALT levels were statistically significant between group II and group V using Mann-Whitney test (p<0,05). In histopatology examination, there were significant differentiation between group I with another group, not only group II but also group III-VII (p<0,05). In the treatment group, group III and IV had been showed the improvement of liver cells damage than group I by using One-way Annova, post hoc Bonferroni (p<0,05).


Conclusion: Uncaria gambir Roxb has hepatoprotector activity start at dose 53 mg/200grBWin rats.  The hepatoprotector activity was not superior than curcuma. 


Keywords: hepatoprotector, Uncaria gambir Roxb, AST,ALT,histopatology

Keywords: hepatoprotector, Uncaria gambir Roxb, AST, ALT, histopatology

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Author Biographies

, Suparni, Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

, Musthari, Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Liza Mutia, Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Mangoloi Sinurat, Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Siti Syarifah, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Dila Sedana, Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Department of Medical Laboratory Technology, Polytechnic Health Ministry of Health Medan, Jl. Williem Iskandar Psr V barat no.6 Medan 20731, Indonesia

Dwi Rita Anggraini, Department of Anatomy, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Department of Anatomy, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Muhammad Ichwan, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara Jl. Dr. Mansur Kampus USU Medan 20155, Indonesia

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Suparni , Musthari , Mutia L, Sinurat M, Syarifah S, Sedana D, Anggraini DR, Ichwan M. The Hepatoprotector Effect of Uncaria gambir Roxb Extract in Wistar Rats Induced by Paracetamol. JDDT [Internet]. 15Dec.2020 [cited 21Jan.2021];10(6-s):61-6. Available from: http://jddtonline.info/index.php/jddt/article/view/4457