Development and Characterization of Elastic Liposomes of Metronidazole for the Treatment of Bacterial Infection
Objective: The objective of present study is to develop and evaluate the elastic liposomes of metronidazole so as to provide the sustained release and improve its bioavailability.
Methods: Elastic liposomes were prepared by rotary evaporation method using Span 80 and Span 60 as a surfactants. The prepared elastic liposomes were evaluated for entrapment efficiency, vesicle size, In vitro drug release.
Results: The drug release profiles from different elastic liposomes-in-vehicle formulations were in agreement with the physicochemical properties of the formulations. The formulation prepared showed an average vesicle size 185.4nm. The amount of drug entrapped into the elastic liposomes formulations was determined. The entrapment efficiency was found to be 73.45±0.78 %. A good amount of drug was entrapped in the liposome formulations prepared. Based on different parameters formulations of batch TG2 was found to be the best formulations. Stability study was performed on the selected formulation TG2. When the regression coefficient values of were compared, it was observed that ‘r’ values of first order was maximum i.e. 0.993 hence indicating drug release from formulations was found to follow Korsmeyer Peppas model release kinetics
Conclusion: These results indicate that elastic liposome can function as probable drug delivery systems to enhance transdermal permeation of metronidazole for treating the topical infections.
Keywords: Metronidazole, Elastic liposomes, Topical administration, Skin infection
2. Drummond DC, Meyer O, Hong K. Pharmacokinetic study of liposome encapsulated drug. Pharmacological reviews 1999; 51(4):691-743.
3. Mezei M, Gulasekharn V. Liposomes a selective drug delivery system for topical route of administration. Lotion dosages form. Life Sci 1980; 26:1473-1477.
4. Touitou E, Junginger, HE Weiner, ND Nagai, T Mezei. Liposomes as a carrier for topical and transdermal delivery. J Pharm Sci 1994; 83:1189-1203.
5. Fresta M, Puglisi G. Application of liposomes as potential cutaneous drug delivery system. In vitro in vivo investigation with radioactivity labeled vesicles. J Drug Target 1996; 4:95-101.
6. Singh S, Vardhan H, Kotla NG, Maddiboyina B, Sharma D, Webster TJ. The role of surfactants in the formulation of elastic liposomal gels containing a synthetic opioid analgesic. Int J Nanomedicine 2016; 11:1475-1482.
7. Sinico C, Fadda AM. Vesicular carriers for dermal drug delivery. Expert Opin Drug Deliv 2009; 6(8):813-825.
8. Lofmark S, Edlund C, Nord CE. Metronidazole is still the drug of choice for treatment of anaerobic infections. Clin Infect Dis 2010; 50 (Suppl 1):S16–23.
9. Lyvers E, Elliott DP. Topical metronidazole for odour control in pressure ulcers. Consult Pharm 2015; 30(9):523–6.
10. Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M. Ethosomes novel vesicular carriers for enhanced delivery: Characterization and skin penetration properties. J Control Release. 2000; 65 (3):403–418.
11. Ainbinder D, Touitou E. A new approach for skin tumor treatment: from delivery system characterization to in vivo evaluation. Drug Deliv Transl Res. 2011; 1(1):53–65.
12. Maheshwari RG, Tekade RK, Sharma PA, Darwhekar G, Tyagi A, Patel RP, Jain DK. Ethosomes and ultradeformable liposomes for transdermal delivery of clotrimazole: A comparative assessment. Saudi Pharm J 2012; 20:161-170.
13. Maurya SD, Prajapati SK, Gupta AK, Saxena GK and Dhakar RC. Formulation development and evaluation of ethosome of stavudine. Indian J Pharm Educ Res 2010; 44(1):102-108.
14. Yasmin Begum M, Abbulu K, Sudhakar M. Design and evaluation of flurbiprofen liposomes. J Pharm Res 2011; 4:653-5
15. Multimer M. Spreadability determination by an apparatus. J Am Pharm Asso 1956; 45:212–214
16. Srinivas MBR, Vishakha K, Prakash P, Jamal BD, Govardhan T, Muni SP. Formulation and in vitro evaluation of liposomes loaded with mupirocin. Int J Res Pharm Nano Sci 2015; 4:162-74.
17. Syeda SS, Krishna SA. Formulation and evaluation of diclofenac sodium transferosomes using different surfactants by thin film hydration method. Pharm Lett 2015; 7:43-53.
18. Sabri, H. S., Alia, W. K., Abdullahb, B. H., Al-Anic, W. M. Formulation design and evaluation of anti-microbial activity of emulgel containing essential oil of Myrtus communis L. Inter J Pharm Sci Rev Res 40:271–277.
19. Benson HA. Transfersomes for transdermal drug delivery. Expert Opin Drug Deliv. 2006; 3(6):727–737.
20. Chen J, Lu WL, Gu W, Lu SS, Chen ZP, Cai BC. Skin permeation behavior of elastic liposomes: role of formulation ingredients. Expert Opin Drug Deliv. 2013; 10(6):845–856.
21. El Maghraby GM, Barry BW, Williams AC. Liposomes and skin: from drug delivery to model membranes. Eur J Pharm Sci. 2008; 34(4–5):203–222.
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