Anti-Inflammatory Potential Evaluation (In-Vitro and In-Vivo) of Arthrophytum scoparium Aerial Part

Anti-Inflammatory Potential Evaluation of Arthrophytum scoparium .

  • Sabrina Manel Kaddour Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria
  • Lekhmici Arrar Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria
  • Abderrahmane Baghiani Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Abstract

Arthrophytum scoparium is plant commonly used in folk medicine in Algeria to treat numerous human diseases especially infectious, Rheumatism, diabetes and cancer. However, its anti-inflammatory activity and the underlying mechanisms have not been studied systematically. The present study aims to assess both the in vitro and in vivo anti-inflammatory potential and antioxidant activity of the plant. Results showed that A. scoparium chloroform extract (ChE) contains the largest amount of phenolic and flavonoid compounds. In-vitro bioassay consisted of investigating the effect of the plant extracts against protein denaturation (BSA and egg albumin). In-vivo anti-inflammatory activity was evaluated by measuring the percentage inhibition of carrageenan-induced rat paw edema after oral administration of the extracts. Results showed that inhibition of protein denaturation was maximum in case of A. scoparium crude extract (CrE) with dose dependant manner. Similarly in the in-vivo study, carrageenan induced inflammation was significantly antagonized by CrE with inhibition of 87.02% at 400 mg/kg. CrE extracts showed the highest scavenger effect against hydroxyl and hydrogen peroxide. The CrE showed potent anti-inflammatory activity in both the conditions in vivo and in vitro. In conclusion different A. scoparium extracts scavenged reactive oxygen species efficiently; it may be due to the presence of its flavonoids and polyphenols. These results support the traditional use of this plant.


Keywords: Arthrophytum scoparium, polyphenols, anti-inflammatory activity, radical scavenging activity.

Keywords: Arthrophytum scoparium, polyphenols, anti-inflammatory activity, radical scavenging activity

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Author Biographies

Sabrina Manel Kaddour, Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Lekhmici Arrar, Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Abderrahmane Baghiani, Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Ferhat Abbas University of Setif, Setif 19000. Algeria

References

1. Chen L, Deng H, Cui H et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017; 9(6):7204-7218.
2. Poovizhi A K, Monika S, Swapna N M, JeyavelKarthick P and Prabhu N. Analysis of anti-inflammatory avtivity in medicinal plants. Int J Recent Sci Res. 2019; 10(09):35039-35045.
3. Yuan H, Ma Q, Ye L and Piao G. The Traditional Medicine and Modern Medicine from Natural Products. Molecules.2016; 21:559.
4. Che T and Zhang. Plant Natural Products for Human Health. Int. J. Mol. Sci. 2019; 20:830.
5. Bailey-Shaw YA, Williams L A D, Green E C, Rodney S, Smith AM. In-Vitro Evaluation of the Anti-Inflammatory Potential of Selected Jamaican Plant Extracts using the Bovine Serum Albumin Protein Denaturation Assay. Int. J. Pharm. Sci. Rev. Res. 2017; 47(1):145-153, No. 27.
6. Oguntibeju O O. Medicinal plants with anti-inflammatory activities from selected countries and regions of Africa. J Inflamm Res.2018; 11:307-317.
7. Allaoui M, Cheriti A, Chebouat E, Dadamoussa B and Gherraf N. Comparative Study of the antioxidant activity andpheols and flavonoids contents of the ethyl acetate extracts from two saharan chenopodacea: Haloxylon scoparium AND Traganum nudatum. Algerian journal of arid environment, 2016; 6(1):71-79.
8. Fatehi N, Allaoui M, Berbaoui H, Cheriti A, Boulenouar N, Belboukhari N. Haloxylon Scoparium: An Ethnopharmacological Survey, Phytochemical Screening and Antibacterial Activity against Human Pathogens Causing Nosocomial Infection. PhytoChem & BioSub Journal. 2017; 11(2).
9. Madoui S, Charef N, Arrar L, Baghianni A and Khennouf S. In vitro Antioxidant Activities of Various Extracts from Flowers-Leaves Mixture of Algerian Cytisus triflorus. Annu Res Rev Biol. 2018; 26(3):1-13.
10. Boussoualim N, Krache I, Baghiani A, Trabssa H, Aouchria S, Arrar L. Human xanthine oxidase inhibitory effect, antioxidant in vivo of Algerian extracts (Globularia alypum L.). J Pharmacogn Phytochem. 2016; 8(4):645-650.
11. Guemmaz T, Zerrargui F, Boumerfeg S, Arrar L, Aoucharia S, Khennouf S, Charef N, Baghiani A. Anti-hemolytic, Anti-lipid Peroxidation, Antioxidant Properties and Toxicity of Xanthium strumarium Leaves Extracts. Annu Res Rev Biol.2018; 24 (3):1-12.
12. Bouaziz A, Khennouf S, Abdalla S, Djidel S, Abu Zarga M, Bentahar A, Dahamna S, Baghiani A, Amira S. Phytochemical Analysis, Antioxidant Activity and Hypotensive Effect of Algerian Azarole (Crataegus azarolus L.) Leaves Extracts. Res.J. Pharm. Biol.Chem. Sci.2014; 8(6):968-976.
13. Mayouf N, Charef N, Saoudi S, Baghiani A, Khennouf S, Arrar L. Antioxidant and anti-inflammatory effect of Asphodelus microcarpus methanolic extracts. J. Ethnopharmacol. 2019; 239:111914.
14. Benslama A, Boumerfeg S, Aouachria S, Guemmaz T, Arrar L, Khennouf S and Baghiani A. Xanthine Oxidase Inhibition, In-vitro Antioxidant Activity and Antibacterial Effect of the Aerial Part Extracts of Thymus pallidus Coss. Curr Nutr Food Sci. 2019; 15:1-9.
15. Rastogi S, Iqbal M S, Ohri D. In vitro study of anti-inflammatory and antioxidant activity of some medicinal plants and their interrelationship. Asian J Pharm Clin Res.2018; 11(4):195-202.
16. Kiranmayi G V N, Anusha V, Chandrika Y, Satya Priya I V, Santhu Swetha K U B G, Vamsi Krishna Y. Preliminary phytochemical screening and in vitro evaluation of anti-inflammatory, antiarthritic, and thrombolytic activities of ethanolic leaf extract of Bauhinia purpurea. Int. J. Green Pharm.2018; 12(1):S241.
17. Kim J, Kim H, Choi H et al. Anti-Inflammatory Effects of a Stauntonia hexaphylla Fruit Extract in Lipopolysaccharide-Activated RAW-264.7 Macrophages and Rats by Carrageenan-Induced Hind Paw Swelling. Nutrients.2018; 10(1):110.
18. Belyagoubi-Benhammou N et al. (Phenolic content and antioxidant activities in vitro of some selected Algerians plants. J Med Plant Res. 2014; 8(40):1198-1207.
19. Kumar V, Roy B K. Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis. Sci. Rep.2018; 8, 10714.
20. Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants (Basel). 2019; 8(4):96.
21. Tijani A A, Adekomi D A, Adewole S O. In vitro and in vivo Determination of Hydroxyl Radical Scavenging Activity (HRSA) of Fractions of Aqueous Extract of Moringa Oleifera Leaves (AEMOL). Eurasian J Med Oncol. 2018; 2(4):209–216.
22. Lupu A R and Cremer L. Hydroxyl radical scanenger activity of natural sod. Roum Arch Microbiol Immunol.2018; 77(1):73-83.
23. Ruskin R, Vasanthakumari B and Citarasu T. In vitro antioxidant activity of various leaf extracts of canthium coromandelicum (Burm.f.) Alston. Asian J Pharm Clin Res.2017; 10:214-218.
24. Gunathilake K D P P, Ranaweera K K D S and Vasantha Rupasinghe H P. In Vitro Anti-Inflammatory Properties of Selected Green Leafy Vegetables. Biomedicine.2018; 6:107.
25. Banerjee S, Chanda A, Adhikari A, Das A, Biswas S. Evaluation of Phytochemical Screening and Anti Inflammatory Activity of Leaves and Stem of Mikania scandens (L.) Wild. Ann Med Health Sci Res. 2014; 4(4):532-536.
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Kaddour SM, Arrar L, Baghiani A. Anti-Inflammatory Potential Evaluation (In-Vitro and In-Vivo) of Arthrophytum scoparium Aerial Part. JDDT [Internet]. 15Sep.2020 [cited 25Sep.2020];10(5):213-8. Available from: http://jddtonline.info/index.php/jddt/article/view/4409