Development and Validation of Advanced UV-Spectrophotometric Methods and a RP-HPLC Method for the Simultaneous Estimation of Beclomethasone Dipropionate and Formoterol Fumarate Dihydrate in Bulk and Pharmaceutical Dosage Forms

  • Rajashree Mashru Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India
  • Manthan Trivedi Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

Abstract

The presented research work aims to develop and validate UV-Spectrophotometric as well as RP-HPLC methods for the simultaneous estimation of Beclomethasone Dipropionate (BDP) and Formoterol Fumarate Dihydrate (FFD). These methods offer a higher degree of sensitivity than the already present methods of analysis. By implementing advanced spectroscopic techniques such as Dual Wavelength Method and First Derivative Spectroscopy it is found that the sensitivity of the methods is increased. The linearity of both the methods was in the range of 10µg/ml to 50µg/ml for BDP and 1µg/ml to 5µg/ml for FFD, with an r2 value of 0.999 and 0.9988 respectively for Dual Wavelength Method and First Derivative Method. The LOD values of Dual Wavelength Method and First Derivative Method were found to be 0.127µg/ml and 0.016µg/ml respectively. A RP-HPLC Method has also been developed and validated for this combination having a linearity in range of 50µg/ml to 250µg/ml for BDP and 1.5µg/ml to 7.5µg/ml for FFD. The r2 value of BDP and FFD was found to be 0.9995 and 0.994. The application of this RP-HPLC Method may also be extended for the simultaneous estimation of a triple combination of Beclomethasone Dipropionate, Formoterol Fumarate and Glycopyrronium Bromide (GPB).


Keywords: Beclomethasone, Formoterol, Glycopyrrolate, Dual-Wavelength-Method, First-Derivative, Spectroscopy, RP-HPLC

Keywords: Beclomethasone, Formoterol, Glycopyrrolate, Dual-Wavelength-Method, First-Derivative, Spectroscopy, RP-HPLC

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Author Biographies

Rajashree Mashru, Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

Manthan Trivedi, Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

Faculty of Pharmacy, G. H. Patel Building, Donor’s Plaza, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

References

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Mashru R, Trivedi M. Development and Validation of Advanced UV-Spectrophotometric Methods and a RP-HPLC Method for the Simultaneous Estimation of Beclomethasone Dipropionate and Formoterol Fumarate Dihydrate in Bulk and Pharmaceutical Dosage Forms. JDDT [Internet]. 15Sep.2020 [cited 25Sep.2020];10(5):108-17. Available from: http://jddtonline.info/index.php/jddt/article/view/4382