Development and Validation of a Green Analytical Method for the Determination of Aspirin and Domperidone Bulk or Formulation Using UV and HPLC
The aim of present study was to investigate the development and validation of a green analytical method for the determination of aspirin and domperidone. Method Development and Validation for Estimation of Domperidone and Aspirin in bulk or formulation by using RP-HPLC. The RP-HPLC method was developed for estimation of Aspirin and Domperidone in synthetic mixture by isocratically using 10 mM KH2PO4: Acetonitrile (20:80) as mobile phase, Prontosil C-18 column (4.6 x 250 mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 231 nm. Then developed method was validated by using various parameters such as, linearity, Range accuracy, precision repeatability, intermediate precision, robustness, limit of detection, limit of quantification. The proposed methods were found to be linear with correlation coefficient close to one. Precision was determined by repeatability, Intermediate precision and reproducibility of the drugs. The robustness of developed method was checked by changing in the deliberate variation in solvent. The result obtained shows the developed methods to be Cost effective, Rapid (Short retention time), Simple, Accurate (the value of SD and % RSD less than 2), Precise and can be successfully employed in the routine analysis of these drugs in bulk drug as well as in tablet dosage form. The Simplicity, Rapidly and Reproducibility of the proposed method completely fulfill the objective of this research work.
Keywords: Asprin; Domperidone; HPLC; Ultra Violet; Validation
2. Saeed AM, Hamzah MJ, Ahmed NQ. Quantitative assay of aspirin and (salicylic acid and heavy metals as impuraties) in Iraqi’s market aspirin tablets using different analytical methods. International Journal of Applied Pharmaceutics, 2018; 10(5):167-172.
3. Willard HH, Merritt LL, Dean JJA, Frank AS. Instrumental method of analysis: CBS Publishers and Distributors, New Delhi, 7th Edition; 1986
4. Mendham J, Denney RC, Barnes JD, Thomas MJ, Chemical analysis, Vogel’s Textbook of Quantitative Chemical Analysis, Pearson Education Asia, Singapore, 6th edn., 2002,1-11.
5. Chatwal GR, Ed AM. Quality Control in Pharmacy; Pharmaceutical Chemistry-Inorganic, Himalaya Publication House, Mumbai, 2nd edn., 1999, 1:25-27.
6. Backett AH, Stenlake JB, Davidson A. Instrumental Method in the development and use of medicines; Practical Pharmaceutical Chemistry, CBS Publishers and Distribution New Delhi, 4th edn., 2002; 2(1-8):85-174.
7. Ahuja S, Scypinski S, Crowther JB. Validation of Pharmaceutical Text Method; Handbook of Modern Pharmaceutical Analysis. Academic Press, London, 2001, 415-445.
8. Sherman RE, Rhodes LJ. Analytical Instrumentation; Practical Guides for Measurement and Control, Instrument Society of America.1996, 647-648.
9. Gennaro AR, Karen BM, Medwick T. Remington ;The Science and Practice of Pharmacy, Mach Publishing Company, Pennsylvania, 19th edn., 1995; 1:437-490.
10. Willard HH, Merritt LL, Dean JA, Settle FA. HPLC Theory and Instrumentation; In Instrumental Methods of Analysis, CBS Publishers and Distributors, New Delhi, 8th edn., 2002, 1-12.
11. Snyder LR, Kirkland JJ, Glajch LJ. Getting Started; In Practical HPLC Method Development, John Willey and Sons, Inc, New York, 2nd edn., 1997,5-17.
12. Snyder LR, Kirkland JJ, Glajch LJ. Non-ionic Samples; Reversed- and Normal-Phase HPLC, In Practical HPLC Method Development, John Wiley and Sons, Inc, New York, 2nd edn. 1997, 233-291.
13. Billiet HAH, Rippel G. Method Development and Selectivity Optimization in High-Performance Liquid Chromatography; In Advances in Chromatography, Marcel Dekker, Inc, New York, 1998; 39:263-310.
14. Sharaf MA. Assessment of Chromatographic Peak Purity; In Advances in hromatography, Market Dekker, Inc, New York, 1997; 37:1-6.
15. Cindy Green. J Vali Teach, In; Analytical method validation. RAC 2000; 6:625- 31.
16. Peter EW. Thin- Layer Chromatography: A modern practical approach. Thomas Graham House, Science Park, Milton Road, Cambridge, UK: The Royal Society of chemistry; 2005.
17. Elhance DN. Foundation of statistics. Kitab mahal, 47th edn; 2003.
18. Ali RMA, Mohammed AAB, Mohammed T, Zainab AJ. Development Method for Determination of Aspirin as Sodium Salicylate by UV-VIS Spectroscopy. IOP Conference Series Materials Science and Engineering. 2019; 571:1-14.
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