Development and Validation of a Green Analytical Method for the Determination of Aspirin and Domperidone Bulk or Formulation Using UV and HPLC

  • Sneha Jagnade Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India
  • Pushpendra Soni Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India
  • Lavakesh Kumar Omray Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Abstract

The aim of present study was to investigate the development and validation of a green analytical method for the determination of aspirin and domperidone. Method Development and Validation for Estimation of Domperidone and Aspirin in bulk or formulation by using RP-HPLC. The RP-HPLC method was developed for estimation of Aspirin and Domperidone in synthetic mixture by isocratically using 10 mM KH2PO4: Acetonitrile (20:80) as mobile phase, Prontosil C-18 column (4.6 x 250 mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 231 nm. Then developed method was validated by using various parameters such as, linearity, Range accuracy, precision repeatability, intermediate precision, robustness, limit of detection, limit of quantification. The proposed methods were found to be linear with correlation coefficient close to one. Precision was determined by repeatability, Intermediate precision and reproducibility of the drugs. The robustness of developed method was checked by changing in the deliberate variation in solvent. The result obtained shows the developed methods to be Cost effective, Rapid (Short retention time), Simple, Accurate (the value of SD and % RSD less than 2), Precise and can be successfully employed in the routine analysis of these drugs in bulk drug as well as in tablet dosage form. The Simplicity, Rapidly and Reproducibility of the proposed method completely fulfill the objective of this research work.


Keywords: Asprin; Domperidone; HPLC; Ultra Violet; Validation

Keywords: Asprin, Domperidone, HPLC, Ultra Violet, Validation

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Author Biographies

Sneha Jagnade, Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Pushpendra Soni, Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Lavakesh Kumar Omray, Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

Radharamn Institute of Pharmaceutical Sciences Bhopal (M.P) 462044 India

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Jagnade S, Soni P, Omray LK. Development and Validation of a Green Analytical Method for the Determination of Aspirin and Domperidone Bulk or Formulation Using UV and HPLC. JDDT [Internet]. 15Nov.2020 [cited 26Nov.2020];10(6):49-6. Available from: http://jddtonline.info/index.php/jddt/article/view/4374