Therapeutic Efficacy of Moxifloxacin Mucoadhesive Hydrogel for Bacterial Keratitis
Bacterial keratitis is a hypothetically devastating corneal infection due to the opportunity of fast development; corneal devastation either to be completed in 24–48 hours with even more contagious bacterial aetiological agents. Moxifloxacin mucoadhesive Hydrogel was prepared by using polymer Hydroxy Propyl Methyl Cellulose E50 LV by hydration method. Moxifloxacin was dissolved in small quantity of water and Benzalkonium Chloride was added to the Polymer solution. The formulations were evaluated for clarity, pH measurement, spread-ability test, drug content estimation, viscosity study, in vitro diffusion study and antibacterial activity. The developed formulation exhibits the sustained release over a period of 10 hour. The optimized formulation was further evaluated with antimicrobial activity. The results of the in-vitro antimicrobial activity of hydrogel were satisfactory.
Keywords: Corneal Infection, Hydrogel, Moxifloxacin, invitro release
2. Robles-Contreras A, Perez-Cano HJ, Babayan-Sosa A, Baca-Lozada O. Bacterial keratitis infection: A battle between virulence factors and the immune response, Common Eye Infections. Imtiaz Chaudhry, IntechOpen, DOI: 10.5772/52264.
3. Leck AK, ThomasPA, Hagan M.et al., Aetiology of suppurative corneal ulcers in Ghana and South India, and epidemiology of fungal keratitis. The British Journal of Ophthalmology.2002; 86 (11):1211–1215.
4. Pinna A, Zanetti S, Sotgiu M, Sechi LA, FaddaG, Carta F. Identification and antibiotic susceptibility of coagulase-negative staphylococci isolated in corneal/external infections. The British Journal of Ophthalmology.1999; 83(7):771–773.
5. ManikandanP, Bhaskar M, Revathy R, JohnRK, Narendran K, NarendranV. Speciation of coagulase-negative Staphylococcus causing bacterial keratitis.Indian Journal of Ophthalmology.2005; 53(1):59–60.
6. Ly AN, PhamJN, BadenochPR, et al. Bacteria commonly isolated from keratitis specimens retain antibiotic susceptibility to fluoroquinolones and gentamicin plus cephalothin,” Clinical and Experimental Ophthalmology.2006; 34 (1):44–50.
7. JohnJF, HarvinAM. History and evolution of antibiotic resistance in coagulase-negative staphylococci: susceptibility profiles of new anti-staphylococcal agents. Therapeutics and Clinical Risk Management.2007; 3(6):1143–1152.
8. Schein OD, BuehlerPO, StamlerJF, VerdierDD, KatzJ. The impact of overnight wear on the risk of contact lens-associated ulcerative keratitis. Archives of Ophthalmology. 1994; 112 (2):186–190,.
9. Limberg MB. A review of bacterial keratitis and bacterial conjunctivitis. Am J Ophthalmol. 1991; 112:2S–9S.
10. Hägerström H.Edsman K. Strømme M. Low-frequency dielectric spectroscopy as a tool for studying the compatibility between pharmaceutical gels and mucus tissue. J. Pharm. Sci. 2003, 92:1869–1881.
11. Hanafy NAN, Quarta A, Ferraro MM, Dini L, Nobile C, De Giorgi ML. Carallo S, Citti C, Gaballo A, Cannazza G,et al. Polymeric Nano-MicellesasNovel Cargo-Carriers for LY2157299 LiverCancerCells Delivery. Int. J. Mol. Sci. 2018; 6:19.
12. Hanafy NAN, El-Kemary M, Leporatti S. Reduction diameter of CaCO3 crystals by using polyacrylic acid might improve cellular uptake of encapsulated curcumin in breast cancer. J. Nanomed. Res. 2018, 7:235–239.
13. Hanafy NA, DeGiorgi MLD, Nobile C, Rinaldi R, Leporatti S. Control of Colloidal CaCO3 suspension by using biodegradable polymers during fabrication. Beni-Suef Univ. J. Basic Appl. Sci. 2015; 4:60–70.
14. Preethi GB, Narendra E. Formulation and Evaluation of In Situ Mucoadhesive Ophthalmic Hydrogel for Sustained Delivery of Pefloxacin Mesylate. International Journal of Pharmacy and Pharmaceutical Sciences. 2015; 7(8):345-350.
15. Gokulgandhi MR, Parikh JR, Megha Barot M, Modi DM. A pH triggered in situ gel-forming ophthalmic drug delivery system for tropicamide. Drug Delivery Technology. 2007; 5:44-9.
16. Zhidong L, Jiawei L, Shufang N, Hui L, Pingtian D, Weisan P. Study of an alginate/HPMC based in situ gelling ophthalmic delivery system for gatifloxacin. Int J Pharm. 2006; 315:12-7.
17. Khanna R, Agarwal S P, Ahuja A. Preparation and evaluation of mucoadhesive buccal films of clotrimazole for oral Candida infections. Indian J Pharm Sci. 1997; 59:299-305.
18. Sultana Y, Aqil M, Ali A, Zafar S. Evaluation of carbopol‐methylcellulose based sustained‐release ocular delivery system for pefloxacin mesylate using rabbit eye model. Pharm Dev Technol. 2006; 11:313‐9.
19. Desi HA, Bhalla HL. Preparation and evaluation of a new eye drop containing a combination of ciprofloxacin and dexamethasone. Indian Drugs. 2000; 37:112.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).