Inhibitory Effect of Xanthine Oxidase from Tamus communis Roots Extracts/Fraction
In the course of our phytochemical studies of plant Tamus communis L., methanol soluble extract (EMeOH) (138 g) was chromatographed on a silica gel column. The column was eluted with chloroform and then with chloroform /methanol mixtures of increasing polarity. A total of 52 fractions (400 ml each) were collected and grouped according to their TLC behaviour into 6 main fractions (I-VI). Total phenolic and flavonoid contents in these extracts were determined by a colorimetric method. Values varied between 73.143±0.009 and 29.214±0.003 equivalent Gallic acid/g lyophilisate. All the extracts showed inhibitory properties on xanthine oxidase, the IC50 ranges from 0.029±0.017 mg/ml to 0.237±0.026 mg/ml. The extracts exhibited an additional superoxide scavenging capacity by using both enzymatic methods and IC50 values range from 0.039±0.023 mg/ml to 0.141±0.086 mg/ml. These results show that Tamus communis L. extracts have strong anti-oxidant effects and may have some clinical benefits.
Keywords: Xanthine oxidase, Antioxidant, Superoxide scavenger, Tamus communis L.
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