Total Phenolic Contents and Antioxidant Properties of Algerian Alkanna tinctoria aerial part Extracts
Objective: Evaluation of the polyphenols, flavonoids and tannins contents of different aqueous extracts of the aerial part of Alkanna tinctoria and their antioxidant activities.
Methodology: Three extracts; decoction, macerate and infusion of the aerial part of Alkanna tinctoria were prepared. The content of total polyphenols, flavonoids and tannins of the extracts was carried out and antioxidant activity was assessed using DPPH (2,2’-diphenyl-1-picryl hydrazyl), β-carotene bleaching and iron chelation tests.
Results : The results showed that Infusion (Inf) was the richest in total polyphenols (189.66 ± 30.77 mg EGA / g of dried extract) and flavonoids (26.80 ± 8.37 mg EQ / g of dried extract). While the content of tannins is almost the same for Dec and Inf (40.27 ± 15.00, 40.25 ± 18.27 mg ETA / g of dried extract). Moreover, the two extracts Inf (IC50 of 0.09 ± 0.0015 mg / ml) and Dec (IC50 of 0.17 ± 0. 031 mg / ml) showed a very strong anti-radical activity towards the radical DPPH. In the β-carotene bleaching test, Inf and Dec showed a significant inhibitory activity of peroxidation with values of 100% and 99.8% and are more effective compared to BHT (98.15%). Dec and Mac showed strong chelating activity with IC50s of 0.003 ± 0.0002 mg / ml and 0.005 ± 0.00008 mg / ml respectively. These chelating activities remain higher than EDTA.
Conclusion: It may be concluded that the extracts of the aerial part of Alkanna tinctoria exhibit significant both content of polyphenols and antioxidant activity.
Keywords: Alkanna tinctoria, polyphenols, flavonoids, antioxidant activity.
Ou J, Wang M, Zheng J, and Ou, S. Positive and negative effects of polyphenol incorporation in baked foods. Food chem. 2019; 284:90-99.
Elsharkawy E, Elshathely M, Abdeljaleel G, Ibrahim Al-johar H. Anti-inflammatory effects of medicinal plants mixture used by Bedouin people in Saudi Arabia. Herba Pol. 2013; 59 (3):76-87.
Salimikia I, Yazdinezhad AR, Golfakhrabadi F, Esfahani HRM. In vitro antioxidant and free radical scavenging activity of four Alkanna species growing in Iran. Pharmacog. Res. 2015; 7 (1):100-104
Kourounakis AP, Assimopoulou AN, Papageorgiou VP, Gavalasa A , Kourounakis PN. Alkannin and Shikonin: Effect on Free Radical Processes and on Inflammation - A Preliminary Pharmacochemical Investigation. Arch. Pharm. Pharm. Med. Chem. 2002; 6:262–266.
Khan UA, H Rahman H, Qasim M , Hussain A, Azizullah Azizllah3, Murad W, Khan Z , Anees M and Adnan M. Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria. BMC Complem Altern. Med. 2015; 15(127):1-6.
Yousefi R, Ghaffarifar F, Dalimiasl A.The Effect of Alkanna tincturia and Peganum harmala extracts on Leishmania major (MRHO/IR/75/ER) in vitro. Iran. J Parasitol. 2009; 4 (1):40-47.
Ferreira A, Proença C, Serralheiro MLM andAraùjo MEM.The in vitro screening for acetylcholinesterase inhibition antioxidant activity of medicinal plants from Portugal. J. Ethnopharmacol. 2006; 108:31-37,.
Khadhri A, El mokhi R et Smiti S. Composé phenoliques et activité antioxydantes de deux extraits de chardon aglu: Atractylis gummifera. Revue Soc. Sci. Nat. de Tunisie. 2013; 39:44-52.
Valentao AP, Fernandes E, Carvalho F, Andrade PB, Seabra RM, and Bastos ML. Studies on the Antioxidant Activity of Lippia citriodora Infusion: Scavenging Effect on Superoxide Radical, Hydroxyl Radical and Hypochlorous. Biol. Pharm. Bull. 2002; 25:1324-1327.
Bentaher A, Khennouf S, Bouaziz A, Baghiani A, Dahamna S, Amira S, Arrar L. Polyphenols Content and Antioxidant Activities of Selected Algerian Plants Used for Gastro-duodenal Ulcers. Der Pharma Chemica 2016 ; 8:88-99.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker AP, Vidal N. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97:654–660.
Gharzouli K, Khennouf S, Amira S and Gharzouli A.Effects of Aqueous Extracts from Quercus ilex L. Root Bark, PunicagranatumL. Fruit Peel and Artemisia herba-alba Asso Leaves on Ethanol-induced Gastric Damage in Rats. Phytother. Res 1999; 13:42-45.
Mayouf N, Charef N, Saoudi S, Baghiani A, Khennouf S, Arrar L. Antioxidant and anti-inflammatory effect of Asphodelus microcarpus methanolic extracts. J. Ethnopharmacol. 2019; 239:1-9.
Amamra S, Charef N, Arrar L, Belhaddad O, Khennouf S, ZAIM K, Baghiani A. Phenolic content, antioxidant and antibacterial effects of fruit extracts of Algerian Ash, Fraxinus excelsior. Indian J. Pharm. Sci. 2018;80(6):1021-1028.
Belkhiri F, Baghiani A, Zerroug MM and Arrar L. Investigation of antihemolytic, xanthine oxydase inhibition,antioxydant and antimicrobial properties of SalviaVerbenacaL. Aerial part extracts. Afr. J. Tradit. Complement. Altern. Med. 2017; 14 (2):273-281.
Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem. 2007; 104:1372–1378.
Ghedadba N, Bousselsela H., Hambaba L, Benbia S et Mouloud Y. Évaluation de l’activité antioxydante et antimicrobienne des feuilles et des sommités fleuries de Marrubium vulgare L. Phytothérapie. 2014; 12:15-24.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Intern. Pharmac. Sc. 2011; 1:98-106.
Laouicha S, Senator A, Kherbache A, Bouriche H. Total phenolic contents and antioxidant properties of Algerian Arbutus unedo L. Extracts. J. Drug Deliv. Ther. 2020; 10(3-s):159-168.
Nur Alam Md, JahanBristi N, Rafiquzzaman Md. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceut. J. 2013; 21:143-152.
Falleh H, Ksouri R, Chaieb K, Karray-BouraouiN,Trabelsi N, Boulaaba M and Abdelly C. Phenolic composition of Cynaracardunculus L. organs, and their biological activities.Comptes Rendus Biologies. 2008 ; 331:372-379.
Albano SM, Miguel MG. Biological activities of extracts of plants grown in Portugal. Indus. Crops Prod. 2010; 1-6.
Laguerre M, Lecomte J, villeneuve P. Evaluation of the ability of antioxidants to counteract lipid oxidation: existing methods, new trends and challengres. Progression in lipid research. 2007; 46(5):244-82.
Ameni DJ, Baghiani A, Boumerfeg S, Dahamna S, Khennouf S, Musa H. Abu zarga and Arrar L. Phytochemical profiles, antioxydants capacity and protective effect against aaph-induced mouse erythrocyte damage by daphne gnidium L. shoot extracts. Int. J Pharm. Pharm. Sci. 2015; 7:148-156.
Gao X, Bjork L, Trajkovski V, Uggla M. Evaluation of antioxidant activities of rosehip ethanol extracts in different test systems. J Sci. Food Agric. 2000; 80:2021- 2027.
Sandhar HK, Kumar B , Prasher S, Tiwari P, Salhan M, Sharma P.A Review of phytochemistry and pharmacology of flavonoids. Internat. Pharmac. Sci. 2011; 1:25-41.
Djeridane A, Yousfi M, Brunel JM, StockerP.Isolation and characterization of a new steroid derivative as a powerful antioxidant from Cleome arabica in screening the in vitro antioxidant capacity of 18 Algerian medicinal plants. Food Chem. Tox. 2010; 48:2599-2606.
Gulcin I. Comparisonof in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids. 2007; 32:431-438.
Sahreen S, Khan MR, Khan RA. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chem. 2010; 122: 1205-1211.
Gülçin I, Elmastat M, Aboul-Enein HY. Determination of Antioxidant and Radical Scavenging Activity of Basil (Ocimum basilicum L. Family Lamiaceae) Assayed by Different Methodologies. Phytother.Res. 2007; 21:354–361.
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