Method Development and Validation for Multicomponent Analysis of Rosuvastatin Calcium and Losartan Potassium in Bulk Drug by RP-HPLC

  • Pramod Kumar Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India
  • Amar Deep Ankalgi Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India
  • Pooja Kaushal Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India
  • Mahendra Singh Ashawat Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Abstract

A simple, sensitive and specific liquid chromatographic method was developed for the simultaneous estimation of Rosuvastatin calcium and Losartan potassium in bulk form. Chromatographic conditions included the  Column C-18 (Shim-pack) 250 x 4.6 mm, particle size 5 µm , mobile phase acetonitrile,  methanol and water pH 3 (orthophosphoric acid) in the ratio 20:25:55 recorded  at 233 nm. The retention time were found to be   3.55 and 4.64 min and average percentage recovery 99.42% and 99.63% for Rosuvastatin calcium and Losartan potassium respectively. The proposed method was found to comply with ICH guidelines.


Keyword: Rosuvastatin calcium, Losartan potassium, RP-HPLC, accuracy and linearity.

Keywords: Rosuvastatin calcium, Losartan potassium, RP-HPLC, accuracy and linearity

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Author Biographies

Pramod Kumar, Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Amar Deep Ankalgi, Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Department of Pharmaceutical Analysis &Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Pooja Kaushal, Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Mahendra Singh Ashawat, Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

Department of Pharmaceutical Analysis & Quality Assurance, Laureate Institute of Pharmacy Kathog Jawalaji, H.P., India

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1.
Kumar P, Ankalgi AD, Kaushal P, Ashawat MS. Method Development and Validation for Multicomponent Analysis of Rosuvastatin Calcium and Losartan Potassium in Bulk Drug by RP-HPLC. JDDT [Internet]. 15Oct.2020 [cited 21Oct.2020];10(5-s):76-4. Available from: http://jddtonline.info/index.php/jddt/article/view/4341