Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts

  • Hind Amira Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • islem Amira
  • Fatima Benchikh
  • Hassiba Benabdallah
  • Walid Mamache
  • Smain Amira


The plants of the genus Phlomis are native to Turkey, North Africa, Europe and Asia.  Phlomis bovei De Noé (Lamiaceae) is a rare Algerian endemic plant, commonly known as Kayat El Adjarah. The objective of this study is to quantify the polyphenol content and to evaluate the antioxidant activities of the ethanolic extract (EthE) of Phlomis bovei De Noé. The plant extract was prepared by macerating 100 g of ground material in 1000 ml of 85 % ethanol for 72 h, then the filtrate was evaporated using a rotary evaporator at a temperature of 45C and the filtrate was dried. The total content in polyphenols was determined using Folin Ciocalteu method. Flavonoid content in extracts was determined using Aluminum trichloride assay and the total content of tannins was determined according to Bate Smith method. The antioxidant activity was investigated in vitro by the DPPH (2, 2-diphenyl-1-picryl-hydrazyl), ABTS and iron chelating assays. The results indicate that the ethanol extract was rich in polyphenols and has an important capacity of scavenging the DPPH and ABTS free radical with an IC50 of 0.05 and 0.018 mg/ml, respectively. However, in the ferrous ions chelating ability test, PBEE extract showed moderate chelating activity (IC50 = 1.59 ± 0.05 mg/ml). To conclude, the obtained results may contribute to add possible scientific data to use Phlomis bovei industrially as herbal medicine and as supplementary sources for natural antioxidant drugs in food.

Keywords: antioxidant activity, ethanol extract, Phlomis bovei De Noé, polyphenol content.

Keywords: antioxidant activity, ethanol extract, Phlomis bovei De Noé, polyphenol content


Download data is not yet available.


[1]. Kumpulainen JT, Salonen JT (1999). Natural Antioxidants and Anticarcinogens in Nutrition, Health, and Disease, The Royal Society of Chemistry, UK pp 178- 187.
[2]. Halliwell B, Gutteridge JMC, Cross CE. Free radicals, antioxidants and human disease: where are we now? J. Lab Clin Med, 1992; 119:598-620.
[3]. Hirasa K, Takemasa M. Spices and herbs: Basic concepts. In Spice Science and Technology New York, USA: Marcel Dekker, Inc. 1998, pp. 9-11.
[4]. Velioglu, Y. S., Mazza, G., Gao, L., & Oomah, B. D. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural Food and Chemistry, 1998; 46:4113–4117
[5]. Quezel P, Santa S. Nouvelle flore de l'Algérie et des régions désertiques meridionales, C.N.R.S, Paris, 1962, p. 805.
[6]. Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem, 2007; 102:771-776.
[7]. Bahorun T, Gressier B, Trotin F, Brunete C, Dine T, Vasseur J, Luyckx M, Cazin M, Pinkas M. Oxygen species scavenging activity of phenolic extract from Hawthorn fresh plant organs and pharmaceutical preparations. ArzneimForsch/Drug Res, 1996; 46:1086-1089.
[8]. Bate-Smith EC. Haemanalysis of tannins, the concept of relative astringency. Phytochemistry, 1973; 12:907-912.
[9]. Re R. Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radical Bio Med, 1999; 26:1231-1237.
[10]. Shekhar TC and Anju G. Antioxidant Activity by DPPH Radical Scavenging Method of Ageratum conyzoides Linn. Leaves. American Journal of Ethnomedicine, 2014; 1(4):244-249.
[11]. Decker EA, Welch B. Role of ferritin as lipid oxidation catalyst in muscle food. J Agr Food Chem, 1990; 36:674-677
[12]. Cao GH, Sofic E, Prior RL.. Antioxidant capacity of tea and vegetables. J Agri Food Chem, 1996; 44:3426-3431.
[13]. Zheng W and Wang S. Antioxidant activity and phenolic composition in selected herbs. J Agr Food Chem, 2001; 49:5165-5170.
[14]. Dehpour AA, Ebrahimzadeh MA, Fazel NS, Mohammad NS. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. GRASAS Y ACEITES, 2009; 60(4):405-412, DOI: 10.3989/gya.010109.
[15]. Benchikh F, Amira S, Benabdallah H. The evaluation of antioxidant capacity of different fractions of Myrtus communisL.leaves. Annual Research & Review in Biology, 2018; 22(5):1-14.
[16]. Mehlous S, Benchikh F, Benabdallah H, Loucif K, Kaoudoune C, Laouer H, Amira S. Evaluation of antioxidant activity and polyphenols content of the hydro-methanolic extract from Saccocalyx satureioides Coss and Dur. Journal of Drug Delivery and Therapeutics, 2020; 10(4):188-190
[17]. Piluzza G and Bullitta S. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area, Pharmaceutical Biology, 2011; 49:3:240-247, DOI: 10.3109/13880209.2010.501083.
[18]. Cai YZ, Luo Q, Sun M Corke . Antioxidant activity and phenolic compounds of 112 Chinese medicinal plants associated with anticancer. Life Sci, 2004; 74:2157-2184
[19]. Soobrattee M, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorum T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mut Res, 2005; 579:200-213.
[20]. Prior RL, Wu X, Schaich K. Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. J Agr Food Chem, 2005; 53:4290-4302.
[21]. Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, Riechel TL. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agr Food Chem, 1998; 46:1887-1892.
[22]. Kaoudoune C, Benchikh F, Benabdallah H, Loucif K, Mehlous S, Amira S. Gastroprotective effect and in vitro antioxidant activities of the aqueous extract from Artemisia absinthium L. aerial parts. Journal of drug delivery and therapeutics, 2020; 10(4):153-156.
[23]. Loucif K, Benabdallah H, Benchikh F, Mehlous S, Ben Souici C, Amira S. Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica. Journal of Drug Delivery and Therapeutics. 2020; 10(3-s):196-198.
1 Views | 0 Downloads
How to Cite
Amira H, Amira islem, Benchikh F, Benabdallah H, Mamache W, Amira S. Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts. JDDT [Internet]. 15Sep.2020 [cited 25Sep.2020];10(5):45-8. Available from: http://jddtonline.info/index.php/jddt/article/view/4339