Evaluation of Protein, Lipid and Inflammatory Profiles in Patients with Non-Hodgkin Lymphoma in the Western Region of Algeria

  • Meriem Rabia Zahzeh Laboratory of Molecular Microbiology, Proteomics and Health University of Sidi Bel Abbes.
  • Touria Zahzeh Laboratory of Molecular Microbiology, Proteomics and Health, University of Sidi Bel Abbes, Algeria

Abstract

Introduction: Non-Hodgkin lymphoma (NHL) is part of the lympho-proliferative syndromes, it is a heterogeneous group of tumors whose incidence has been increasing in recent years. A disruption of protein and lipid profiles is verified during this pathology as well as an increase in the levels of specific markers of inflammation.


Objective: Our study aims to explore protein and lipid profiles and highlight an inflammatory syndrome via the assay of parameters of acute inflammation in patients with NHL and healthy subjects in western Algeria.


Methods: A case-control study comprising 100 patients with NHL and 40 healthy subjects was carried out. Protein and lipid profiles were respectively studied by assays for total protein and globulins and analysis of cholesterol and triglyceride levels. The inflammatory status was demonstrated by studying two parameters: C reactive protein (CRP) and sedimentation rate (VS).


Results: Our results demonstrated an increase in alpha1-globulin and a decrease in gamma-globulin in patients compared to controls (p <0.05). The lipid profile study showed no significant difference for cholesterol but a significant increase in triglycerides in patients with NHL (p <0.05). A highly significant difference in CRP and VS levels between our two groups (p <0.01) with a marked increase in patients demonstrated the presence of acute inflammatory syndrome.


Conclusion: This study allowed us to highlight the alterations in protein and lipid profiles found in NHL and the presence of an inflammatory state, involved in the pathophysiology of non-Hodgkin lymphoma in particular.


Keywords: non-Hodgkin lymphoma, protein profile, lipid profile, CRP, VS.

Keywords: non-Hodgkin lymphoma, protein profile, lipid profile, CRP

Downloads

Download data is not yet available.

Author Biographies

Meriem Rabia Zahzeh, Laboratory of Molecular Microbiology, Proteomics and Health University of Sidi Bel Abbes.

Laboratory of Molecular Microbiology, Proteomics and Health, University of Sidi Bel Abbes, Algeria

Touria Zahzeh, Laboratory of Molecular Microbiology, Proteomics and Health, University of Sidi Bel Abbes, Algeria

Laboratory of Molecular Microbiology, Proteomics and Health, University of Sidi Bel Abbes, Algeria

References

1. Louanchi L, Allouda M, Ait Ameur N, et al, « Syndromes lymphoprolifératifs hors myélomes Aspects cliniques » Hématologie, 2015; 21(1):46-67.
2. Hordé P, « Lymphome (cancer du système lymphatique) symptômes et traitement », 2016; 206-218.
3. Boudjerra N, Oukid S, Abad MT and al, « Etude descriptive de 2915 cas de lymphomes non hodgkinien ganglionnaires de l’adulte. Période 2007-2012 » Revue algérienne d’hématologie, 2015; 10-11.
4. Ahossi V, Boisramé-Gastrin S, Perrot G, and al, «Non-hodgkinson lymphoma with mandibular localisation: two case histories» Med Buccale Chir Buccale, 2006; 12(1):21-29.
5. Zeggai S, Harir N, Touhami H, and al, « Profil histoépidémiologique des lymphomes des adultes dans l’Ouest algérien: à propos de 1 544 cas» African Journal of Cancer, 2013; 5:127-132.
6. Moussati S and Aina M, « Etude des facteurs associés au risque de lymphome malin non Hodgkinien ». Tlemcen, Algérie. 2016.
7. Dupuy AM, Badiou S, Descomps B and al, « Dosage immunoturbidimètrique de la CRP et de la CRP haute sensibilité sur sérum et plasma hépariné : apport des réactifs olympus » Spectra biologie, 2003; 132:44-46.
8. Evrard B, Roszyk L, Fattal S and al, « Evaluation de l’Actim CRP : test rapide de dosage semi- quantitatif de la CRP sur le sang total » Annales de biologie clinique, 2005; 5.
9. Janeway CA, Travers P, Duverlie G and al. Immunobiologie. Paris : Masson ; 2003.
10. Bletry O, Khan JE and Somogyi A. Immunopathologie, réaction inflammatoire. Paris: Masson, 2006.
11. Boukhari AA, Hartani M, Boumesbah S, « Etude de la corrélation entre la vitesse de sédimentation et la protéine C réactive dans la recherche de syndrome inflammatoire » Hématologie, 2019.
12. Kuzu OF, Noory MA and Robertson GP, «The Role of Cholesterol in Cancer» Cancer research. 2016; 76(8):2063-2070.
13. Ding X, Zhang W, Li S, and al, «The Role of Cholesterol Metabolism in Cancer» Suis J Cancer Res, 2019; 9(2):219-227.
15. Allioua M. « Statut nutritionnel et désordres métaboliques chez les patients atteints de lymphome non hodgkinien ». Tlemcen, Algérie, 2011.
15. Skeggs LT Jr and Hochtrasser H, «Multiple automatic sequential analyses» Clin Chem, 1964.
16. Aribi M, « Eléctrophorèses de zone des proteines sériques sur acétate de cellulose : méthode densitométrique (HELENA) ». Tlemcen, Algérie, 2007.
17. Anonyme, « CRP-Latex, slide agglutination ». Spain : Spinreact S.A, 2005.
18. Borghini T, Vernez L, Kesseler D, « Fiche technique protéine C réactive et vitesse de sédimentation». Centre suisse de contrôle de qualité, 2013.
19. Natio HK, « Cholesterol » Clin Chem The C.V. Mosby Co. St Louis. Toronto. Princeton, 1984; 1194-11206.
20. Fossati P and Prencipe L, «Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide» Clin Chem, 1982; 28:2077-80.
21. Kaplan A and al, «Triglycerides» Clin Chem the C.V. Mosby Co. St Louis. Toronto. Priceton, 1984; 1194-1206.
22. Boussarie D. L'électrophorèse des protéines sériques en pathologie du furet (Mustela putorius furo). France : Bull. Acad. Vét ; 2007.
23. Bouayadi O, Bensalah M, Rahmani N and al, «Electrophorèse des protéines sériques: étude de 410 profils électrophorétiques» Pan Afr Med J, 2019; 32:161.
24. Gennes C. Syndromes inflammatoires. Traité de médecine interne. Flammarion ; 1996. P. 98-102.
25. Balcells A. Examen de laboratoire pour le praticien. Paris : Masson ; 1998.
26. Thomas C. Interprétation de l’électrophorèse des protéines sériques. F.M.C Tourcoing, 2015.
27. Frenzel L, « L’hypogammaglobulonémie : que faut-il en retenir ? » Feuillets de biologie, 2017.
28. Grivennikov SI, Greten FR, Karin M, «Immunity, Inflammation, and Cancer » Cell, 2010; 140(6):883-99.
29. Boras E, Slevin M, Alexander MY and al, «Monomeric C-reactive protein and Notch-3 co-operatively increase angiogenesis through PI3K signalling pathway» Cytokine, 2014; 69:165-79.
30. Guyton KZ, Xu Q, Holbrook NJ, «Induction of the mammalian stress response gene GADD153 by oxidative stress: role of AP-1 element» Biochem J, 1996; 314(2):547-54.
31. Kim Y, Ryu J, Ryu MS and al, «C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression» FEBS Lett, 2014; 588(4):625-31.
32. Tugal D, Liao X, Jain MK, «TIS21 (/BTG2/PC3) as a link between ageing and cancer: cell cycle regulator and endogenous cell death molecule» Arterioscler Thromb Vasc Biol, 2013; 33:1135-44.
33. Marnella L, Moldb C, Du Clos TW, « C-reactive protein: Ligands, receptors and role in inflammation» Clinical Immunology, 2005; 117(2):104-111.
34. Devaraj S, Du Clos TW, Jialal I, « Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects» Arterioscler Thromb Vasc Biol, 2005; 26:1359-63.
35. Sproston NR and Ashworth JJ, « Rôle de la protéine C-réactive sur les sites d'inflammation et d'infection » Front Immunol, 2018; 9: 754.
36. Felger JC, Haroon E, Patel TA and al, «What does plasma CRP tell us about peripheral and central inflammation in depression?» Molecular Psychiatry, 2020; 25:1301-1311.
37. Legouffe E, Rodriguez C, Picgt MC and al, «C-Reactive Protein Serum Level is a Valuable and Simple Prognostic Marker in Non Hodgkin's Lymphoma» Leukemia & Lymphoma, 1998; 31:3-4:351-357.
38. Brigden M, «The erythrocyte sedimentation rate. Still a helpful test when used judiciously» Postgrad Med, 1998; 103.
39. Monti M, « Vieux et nouveaux biomarqueurs inflammatoires : quelle utilité pour l’interniste généraliste ? » Rev Med Suisse, 2013; 9:2008-2012.
40. Durant R, Blain H, Jeandel C, « Syndrome inflammatoire chez la personne âgée » Traité EMC AKOS (Traité de Médecine), 2005.
41. Pierre AS. Le métabolisme des acides gras monoinsaturés et la prolifération des cellules cancéreuses coliques : rôle de la Stéaroyl-CoA Désaturase-1 et effets des isomères conjugués de l’acide linoléique. Université de Bourgogne; 2012.
42. Raynard B, Barnoud D, Schneider SM and al. Nutrition et hémopathies malignes. Traité de nutrition artificielle de l’adulte. La société francophone de nutrition entérale et parentérale ; 2007. P.777-785.
43. Lagarde L, « Métabolisme des lipides bio-actifs. Pathologie Biologie » Edition scientifique et medicals Elsevier SAS, 2003; 51:241-243.
44. Weinroth SE, Parenti DM, Simon GL, «Wasting syndrome in AIDS: pathophysiologic mechanisms and therapeutic approaches» Infect Agents Dis, 1995; 4:76-94.
45. Swinnen JV, Vanderhoydonc F, Elgamal AA and al, « Selective activation of the fatty acid synthesis pathway in human prostate cancer» Int J Cancer, 2000; 88:176-179.
46. Yoon S, Lee MY, Park SW and al, « Up-regulation of acetyl-CoA carboxylase alpha and fatty acid synthase by human epidermal growth factor receptor 2 at the translational level in breast cancer cells» J Biol Chem, 2007; 282:26122-26131.
47. Swinnen JV, Brusselmans K, Verhoeven G, « Increased lipogenesis in cancer cells: new players, novel targets» Curr Opin Clin Nutr Metab Care, 2006; 9:358-365.
48. Sakai K, Okuyama H, Yura J and al, « Composition and turnover of phospholipids and neutral lipids in human breast cancer and reference tissues» Carcinogenesis, 1992; 13:579-584.
49. Robert J, Montaudon D, Hugues P, « Incorporation and metabolism of exogenous fatty acids by cultured normal and tumoral glial cells» Biochim Biophys Acta, 1983; 752:383-395.
Statistics
5 Views | 6 Downloads
How to Cite
1.
Zahzeh MR, Zahzeh T. Evaluation of Protein, Lipid and Inflammatory Profiles in Patients with Non-Hodgkin Lymphoma in the Western Region of Algeria. JDDT [Internet]. 15Sep.2020 [cited 25Sep.2020];10(5):1-. Available from: http://jddtonline.info/index.php/jddt/article/view/4335