Formulation and Cytotoxic Characterization of Trigonella foenum Loaded Polymeric Nanoparticles
The aim of present study was to formulate herbal Trigonella Foenum (TF) loaded nanoparticles using hydrophilic polymer at different concentration and optimizing the nanoparticle formulation. Herbal drug having high molecular size or poor water solubility, suffers from less assimilation and poor bio accessibility and hence loading of TF in nanoparticles improves the systematic drug delivery approaches. The formulation containing 3 % of polymer PVP showed optimum result with encapsulation efficiency of 77.73±0.28 and loading efficiency of 16.98±0.7. Simultaneously showing high stability with zeta potential of -23.7±0.98 mV and PDI of 215. The mean average particle size of nanoparticles was found to be 432.1 ± 16.01. FT-IR studies suggested compatibility of polymer and drug when observed with major peaks and functional groups. The in-vitro SRB cytotoxicity assay showed a maximum inhibition of cell viability of 71±2.3 % and IC 50 value of 100±10.2 ug/ml.
Keywords: Trigonella Foenum, poor bioavailability, polymeric nanoparticle , cytotoxicity assay
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