In-vitro and in-vivo anti-inflammatory activity of Plumeria indica Linn flowers
Inflammation is a reaction of a living vascularised tissue to an injury. Conventional or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it sometimes have serious side effects. So, number of herbal medicines is recommended for the treatment of inflammation that has no side effects. Hence our study focused to investigate the physicochemical, qualitative phytochemical analysis of bioactive compounds and In-vitro and In-vivo anti-inflammatory activity of Plumeria indica Linn (P. Indica) flowers extract which has boundless medicinal properties. The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The aqueous and ethanolic extract of P. Indica flowers was screened for in-vivo anti-inflammatory activity by carrageenan induced paw edema in rat model and in-vitro anti-inflammatory activity by human red blood cell membrane stabilization method. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids ect. Ethanolic extract showed best in vitro anti-inflammatory activity was screened for in vivo anti-inflammatory activity at the dose level of 250 and 500mg/kg. Indomethacin at the dose level of 10 mg/kg was used as reference standard drug. Both the extracts showed a dose dependent anti-inflammatory potential which provide scientific basis for the traditional claims of P. Indica flowers as an anti-inflammatory drug.
Keywords: Plumeria indica Linn, Anti-inflammatory activity, Carrageenan, Human red blood cells membrane
2. Rang HP, Dale MM, Ritter JM. Anti-inflammatory and immunosuppressant drugs, chapter 14. Flower RJ; Rang and Dale’s Pharmacology, 6th Ed. Elsevier Publication. 2008; 226-45.
3. Sangita K, Shukla G, Sambasiva Rao A. The present status of medicinal plants-Aspects and prospects. Int J Res Pharm Biomed Sci 2011; 2:19-22.
4. Karim A, Sohail MN, Munir S, Sattar S. pharmacology and phytochemistry of Pakistani herbs and herbal drugs used for treatment of diabetes. Int J Pharmacol 2011; 7:419-39.
5. Pattari LS, Muchandi VN, Haricharan KN, Himabindu GM, Tejaswi CH, Ramanjaneyulu K, et al. Study of analgesic acxtivity of Litsea glutinosa (L.) ethanolic extract on swiss albino mice. Int J Pharm Sci Res 2010; 1:93-7.
6. WHO. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine WHO/EDM/TRM/2000. 1, Geneva, Switzerland, 2000.
7. Mulla WA, More SD, Jamge SB, Pawar AM, Kazi MS, Varde MR. Evaluation of antiinflammatory and analgesic activities of ethanolic extract of roots Adhatoda vasica Linn. Int J Pharm Tech Res 2010; 2:1364-8.
8. Shinde P. R., Patil P. S., Bairagi V. A. Phytopharmacological Review of Plumeria species. Sch Acad J Pharm 2014; 3(2):217-227.
9. The Unani pharmacopeia of India. 2007–2010. Department of AYUSH, Ministry of health and family welfare, Government of India.
10. Nayak A, Garg M, Jain S, Khan MA, Jain DK, Khan N. Anti-Urolithiatic and invitro-invivo anti-oxidant effects of methanolic extract of thunbergia laurifolia on ethylene glycol-induced kidney calculi in rats. Sch Acad J Pharm 2019; 8 (3):94-104.
11. Jain DK, Nayak A, Patel P, Jain A, Khan MA. Appraisal of in vitro antioxidant and in vivo anti-inflammatory activities of various extracts from the fruits of vitis vinifera L. Sch Acad J Pharm 2019; 8 (3):86-93.
12. Jain DK, Patel NS, Nagar H, Patel A, Chandel HS. Anti-arthritic activity of tridax procumbens ethanolic extract of leaves. RGUHS J Pharm Sci 2012; 2(4):80-86.
13. Guideline Document on Acute oral Toxicity Testing, Series on Testing and Assessment No. 423. Paris: Organization for Economic Co-Operation and Development, OECD Environment, Health and Safety Publications; 1996. Available from: http//www.oecd.org/ehs.
14. Jonsson M, Jestoi M, Nathanail AV, Kokkonen UM, et al. Application of OECD Guideline 423 in assessing the acute oral toxicity of moniliformin. Food Chem Toxicol, 2013; 53:27-32.
15. Sadique J, Al-Rqobah NA, Bughaith MF, El-Gindy AR. The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia, 1989; LX: 525-532.
16. Oyedapo OO, Akinpelu BA, Orefuwa SO. Anti-inflammatory effect of Theobroma cacao, root extract. J Trop Med Plants, 2004; 5:161-166.
17. Ahmed F, Selim MST, Das AK, Choudhuri MSK. Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn. Pharmazie, 2004; 59(4):329-30.
18. Gupta S, Kohli S, Dwivedi S. In-Vitro anti-inflammatory activity of Sarcostemma acidum Wight. & Arn. Indian herb by Human red blood cell membrane stabilization method. Int J Pharm Teach Prac 2011; 2(4):184-188.
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