Costus afer modulates the activities of glycolytic and gluconeogenic enzymes in streptozotocin induced diabetic rats

  • Armelle D Tchamgoue Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaounde https://orcid.org/0000-0002-3431-2059
  • Paul DD Dzeufiet Laboratory of Animal Biology, University of Yaounde, P.O. Box 812 Yaounde, Cameroon
  • Jules-Roger Kuiata Research Unit of Microbiology and Antimicrobial Substances, University of Dschang, P.O Box 67 Dschang, Cameroon https://orcid.org/0000-0002-5134-9427
  • Gabriel A Agbor Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé Cameroon

Abstract

Background: Glycolytic enzymes (Hexokinase and α-glucosidase) and gluconeogenic enzymes (Glucose-6-phosphatase and fructose-1,6-disphosphatase) control carbohydrate metabolism and hence glucose hemostasis, an important approach for the control of diabetes. The present study was carried out to evaluate the effect of hydro-ethanolic leaf extract of Costus afer on these enzymes.


Materials and methods: Streptozotocin-induced diabetic rats were administered hydro-ethanolic leaf extract of Costus afer (250, 500 and 1000 mg/kg body weight) orally for 8 weeks. Effect of the extract was monitored on the decrease in glycemia and the modulatory activities on glycolytic and gluconeogenic enzymes activities. The pancreas was also immunohistochemically examined for regeneration of the Islet cells.


Results: Hydro-ethanolic extract significantly decreased the blood glucose concentration, serum glucose-6-phosphatase and fructose-1,6-disphosphatase activities which were associated with an increase in the activity of hexokinase in the liver and kidney. immunohistochemical examination of the pancreas, revealed the preservation and regeneration of the islet of the Langerhans cells. 


Conclusion: Thus, C. afer acts against hyperglycemia modulating the activities of glucose-6-phosphatase, fructose-1,6-bisphosphatase and hexokinase, and regeneration of β-cells.

Keywords: Costus afer, gluconeogenic enzymes, glycolytic enzymes, streptozotocin, α-glucosidase

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Author Biographies

Armelle D Tchamgoue, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaounde

Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé Cameroon

Paul DD Dzeufiet, Laboratory of Animal Biology, University of Yaounde, P.O. Box 812 Yaounde, Cameroon

Laboratory of Animal Biology, University of Yaounde, P.O. Box 812 Yaounde, Cameroon

Jules-Roger Kuiata, Research Unit of Microbiology and Antimicrobial Substances, University of Dschang, P.O Box 67 Dschang, Cameroon

Research Unit of Microbiology and Antimicrobial Substances, University of Dschang, P.O Box 67 Dschang, Cameroon

Gabriel A Agbor, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé Cameroon

Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé Cameroon

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Tchamgoue A, Dzeufiet P, Kuiata J-R, Agbor G. Costus afer modulates the activities of glycolytic and gluconeogenic enzymes in streptozotocin induced diabetic rats. JDDT [Internet]. 15Aug.2020 [cited 23Apr.2021];10(4-s):63-0. Available from: http://jddtonline.info/index.php/jddt/article/view/4270