Metal Chelating and Cupric Ion Reducing Antioxidant Capacities of Ammoides atlantica Aqueous Extract

  • Karima Loucif Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Hassiba Benabdallah Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Fatima Benchikh Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Soulaf Mehlous Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Chahrazed Kaoudoune Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Chawki Ben Souici Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria
  • Smain Amira Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Abstract

Reactive oxygen (ROS) and nitrogen species (RNS) are produced in all cells and play important roles in physiology. The loss of the redox balance, either by an increase of oxidant molecules ROS and RNS or by decreased antioxidant system activities cause a state of oxidative stress. Several studies are going on worldwide directed towards finding natural antioxidants of plant origin. Plants containing phenolic compounds have been reported to possess strong antioxidant activity. The objective of this study is to evaluate total polyphenols and flavonoids contents (TPC and TFC) as well as examine the in vitro antioxidative properties from aqueous extract of Ammoides atlantica (AqE). TPC was estimated utilizing Folin-Ciocalteu's reagent. TFC was evaluated utilizing the aluminum chloride method. The antioxidant properties were evaluated using metal chelating and cupric ion reducing antioxidant capacity (CUPRAC) assays. Indeed, results showed that the AqE is rich in polyphenols (141.74±0.44 µg gallic acid equivalents/ mg of dry weight), and flavonoids (61.87±6.7 µg quercetin equivalent/ mg dry weight). These phytochemical compounds possess significant antioxidant activities. The results showed that AqE exhibited a good Metal chelating activity with an IC50 of 36.57±4.73 µg/ mL. CUPRAC assay showed that AqE extract exhibited high cupric ion reducing antioxidant capacity with an A0.5 of 8.58±0.13 µg/mL. These findings provide evidence that AqE of Ammoides atlantica is a potential source of antioxidant which have many benefits towards human health.


Keywords: Ammoides atlantica, aqueous extract, phenolic compounds, metal chelating and cupric ion reducing antioxidant capacity.

Keywords: Ammoides atlantica, aqueous extract, phenolic compounds, metal chelating and cupric ion reducing antioxidant capacity

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Author Biographies

Karima Loucif, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Hassiba Benabdallah, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Fatima Benchikh, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Soulaf Mehlous, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Chahrazed Kaoudoune, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, Badji Mokhtar University, 23000-Annaba, Algeria.

Chawki Ben Souici, Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria

Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria

Smain Amira, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

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1.
Loucif K, Benabdallah H, Benchikh F, Mehlous S, Kaoudoune C, Souici CB, Amira S. Metal Chelating and Cupric Ion Reducing Antioxidant Capacities of Ammoides atlantica Aqueous Extract. JDDT [Internet]. 15Aug.2020 [cited 26Feb.2021];10(4-s):108-11. Available from: http://jddtonline.info/index.php/jddt/article/view/4245