Study of the Chelating Ability of Hexane, Chloroform, Ethyl acetate and Methanolic Root Extracts from Algerian Phragmites australis Species

  • Abdelhakim Sellal Department of Biology and Plant Ecology, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria
  • Rima Belattar Department of Biochemistry, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria

Abstract

Introduction: Heavy metals that enter organisms can cause illness and disease. Chelation therapy is a solution that uses chelating agents to protect these organisms by high accumulation. Materials and methods: The objective of this study is to evaluate the capability of different roots extracts from reed or EDTA (as standard) to complexate iron using, ferrozine-ferrous reducing assay or Zn and Cu using the murexide-Zn or Cu reducing assay in vitro. Results: the results proved that the organic phase of the first extract and EDTA (standard chelator), have the strongest chelation activities with respective rates of 70% and 97% for iron, 55% and 56% for zinc and 47% and 88% for copper compared to the control groups considered to be 100% of the complexation. Finally, the HPLC analysis showed the presence of six organic acids, which are probably responsible for this effect. Conclusion: The hexane extract and EDTA (standard), have the strongest chelation activities.


Keywords: Phragmites australis, Chelation, Heavy metals, Organic acids, Ferrozine, Murexide.

Keywords: Phragmites australis, Chelation, Heavy metals, Organic acids, Ferrozine, Murexide

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Author Biographies

Abdelhakim Sellal, Department of Biology and Plant Ecology, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria

Department of Biology and Plant Ecology, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria

Rima Belattar, Department of Biochemistry, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria

Department of Biochemistry, Faculty of Nature Sciences and Life, Ferhat Abbas University, Setif 1, Algeria

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Sellal A, Belattar R. Study of the Chelating Ability of Hexane, Chloroform, Ethyl acetate and Methanolic Root Extracts from Algerian Phragmites australis Species. JDDT [Internet]. 15Jul.2020 [cited 7Aug.2020];10(4):87-2. Available from: http://jddtonline.info/index.php/jddt/article/view/4210