Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

  • Saliha Laouicha Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria
  • Abderrahmane Senator Faculty of Natural and Life Sciences, University of Batna 2, Batna, 5000, Algeria
  • Abdallah Kherbache Department of Microbiology and Biochemistry, Faculty of Sciences, University Mohamed Boudiaf, M’sila, M’sila, 28000, Algeria
  • Hamama Bouriche Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria

Abstract

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH, OH, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH, OH, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications.
Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.
Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo

Downloads

Download data is not yet available.

Author Biographies

Saliha Laouicha, Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria

Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria

Abderrahmane Senator, Faculty of Natural and Life Sciences, University of Batna 2, Batna, 5000, Algeria

Faculty of Natural and Life Sciences, University of Batna 2, Batna, 5000, Algeria

Abdallah Kherbache, Department of Microbiology and Biochemistry, Faculty of Sciences, University Mohamed Boudiaf, M’sila, M’sila, 28000, Algeria

Department of Microbiology and Biochemistry, Faculty of Sciences, University Mohamed Boudiaf, M’sila, M’sila, 28000, Algeria

Hamama Bouriche, Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria

Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif 1, Setif, 19000, Algeria

References

[1]: Inbathamizh L, Ponnu TM, Mary EJ, ʺIn vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticlesʺ, J. Pharm. Re. 2013; 6:32-38.
[2]: Lobo V, Patil A, Phatak A, Chandra N, ʺFree radicals, antioxidants and functional foods: impact on human healthʺ, Pharmacogn Rev. 2010; 4:118-126.
[3]: Gönenç A, Hacışevki A, Tavil Y, Çengel A, Torun M, ʺOxidative stress in patients with essential hypertension: a comparison of dippers and nondippersʺ, Eur J Intern Med. 2013; 24: 139-144.
[4]: Ramana KV, Srivastava S, Singhal SS, ʺLipid peroxidation products in human death and diseaseʺ, Oxid Med Longev. 2013; 2013:1-3
[5]: Ozsoy N, Can A, Yanardag R, Akev N, ʺAntioxidant activity of Smilax excelsa L. leaf extractsʺ, Food Chem. 2008; 110: 571-583.
[6]: Rice-Evans C, Miller N, Paganga G, ʺAntioxidant properties of phenolic compoundsʺ, Trends Plant Sci. 1997; 2(4):152-159.
[7]: El Haouari M, L´opez JA, Mekhfia H, Rosado JJ, Salido GM, ʺAntiaggregant effects of Arbutus unedo extracts in human plateletsʺ, J. Ethnopharmacol. 2007; 113:325-331
[8]: Ziyyat A, Legssyer A, Mekhfi A, Dassouli A, Serhrouchni M, Benjelloun J, ʺPhytotherapy of hypertension and diabetes in oriental Moroccoʺ, J. Ethnopharmacol. 1997; 58:45-54.
[9]: Çubukçu B, Suzgeç S, Geleneksel Folklorik Droglar Dergisi, 1997; 4:4.
[10]: Pallauf K, Rivas-Gonzalo JC, del Castillo MD, Cano MP, de Pascual-Teresa S, ʺCharacterization of the antioxidant composition of strawberry tree (Arbutus unedo L.) fruitsʺ, J. Food Compos. Anal. 2008; 21:273-281.
[11]: Benahmed M, Djeddi N, Akkal S, Laouar H, ʺSaccocalyx satureioides as corrosion inhibitor for carbon steel in acid solutionʺ, Int J Ind Chem. 2016; 7:109-120
[12]: Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y, ʺEvaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgaeʺ, Food Chem. 2007; 102:771-776.
[13]: Bahorun T, Gressier B, Trotin F, Brunete C, Dine T, Vasseur J, Gazin JC, Pinkas M, Luycky M, Gazin M, ʺOxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparationsʺ, Arzneimittelforschung.1996; 46: 1086-1089.
[14]: Que F, Mao L, Pan X, ʺAntioxidant activities of five Chinese rice wines and the involvement of phenolic compoundsʺ, Food Res Int. 2006; 39:581-587.
[15]: Sudha G, Sangeetha Priya M, Indhu Shree R, Vadivukkarasi S, ʺIn vitro free radical scavenging activity of raw pepino fruit (solanum muricatum aiton) ʺ, Int. J. Curr. Pharm. Res. 2011; 3:137-140.
[16]: Lee DS, Woo JY, Ahn CB, Je JY, ʺChitosan–hydroxycinnamic acid conjugates: Preparation, antioxidant and antimicrobial activityʺ, Food Chem. 2014; 148:97-104
[17]: Rohit Y, Saurabh R, Ajay S, ʺReview on antioxidants activity and its evaluationʺ. J. Pharma. Res. 2012; 1(2):41-58.
[18]: Bougatef A, Hajji M, Balti R, Lassoued I, Triki-Ellouz Y, Nasri M, ʺAntioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteasesʺ, Food Chem. 2009; 114: 1198-1205.
[19]: Bouhlali EDT, Alem C, Filali Zegzouti Y, ʺAntioxidant and anti-hemolytic activities of phenolic constituents of sixmoroccan date fruit (Phoenix dactylifera L.) syrupsʺ, Biotechnol. Indian J. 2016; 12(1): 45-52.
[20]: Pabuçcuoğlu A, Kıvcak B, Bas M, Mert T, ʺAntioxidant activity of Arbutus unedo leavesʺ, Fitoterapia. 2003; 74: 597-599.
[21]: Jiang YH, Jiang XL, Wang P, Hu XK, ʺIn vitro antioxidant activities of water-soluble polysaccharides extracted from isaria farinosa b05ʺ, J. Food. Biochem. 2005; 29:323-335.
[22]: Nagulendran KR, Velavan S, Mahesh R, Hazeena Begum V, ʺIn Vitro antioxidant activity and total polyphenolic content of Cyperus rotundus Rhizomesʺ, E-J. Chem. 2007; 4:440-449.
[23]: Serhat K, Sait C, Semra T, Ökkes Y and Ismail T, ʺHydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthornʺ, Chem. J. 2012; 02:9-12.
[24]: Gülçin ˙I, Alici HA, Cesur M, ʺDetermination of in Vitro Antioxidant and Radical Scavenging Activities of Propofolʺ, Chem. Pharm. Bull. 2005; 53(3):281-285.
[25]: Yin H, Xu L, Porter NA, 2011. ʺFree radical lipid peroxidation: mechanisms and analysisʺ, Chem Rev. 2011; 111:5944-5972.
[26]: Gonçalves S, Gomes D, Costa P, Romano A, ʺThe phenolic content and antioxidant activity of infusions from Mediterranean medicinal plantsʺ. Ind Crops Prod. 2013; 43:465-471.
[27]: Ben Jeddou K, Chaari F, Maktouf S, Nouri-Ellouz O, Helbert CB, Ghorbel RE, ʺStructural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peelsʺ, Food chem. 2016; 205: 97-105.
[28]: Balti R, Bougatef A, El Hadj Ali N, Ktari N, Jellouli K, Nedjar-Arroume N, Dhulster P, Nasri M, ʺComparative Study on Biochemical Properties and Antioxidative Activity of Cuttlefish (Sepia Officinalis) Protein Hydrolysates Produced by Alcalase and Bacillus licheniformis NH1 Proteasesʺ, J. Amino Acids, 2011; 2011: 1-11.
[29]: Zhao H, Zhang H, Yang S, ʺPhenolic compounds and its antioxidant activities in ethanolic extracts from seven cultivars of Chinese jujubeʺ Food Sci. Hum. Wellness. 2014; 3:183-190.
[30]: Gülçin İ, Beydemir Ş, ʺPhenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitorsʺ Mini-Rev. Med. Chem. 2013; 13:408-430.
[31]: Carcache-Blanco EJ, Cuendet M, Park EJ, Su BN, Rivero-Cruz JF, Farnsworth NR, Pezzuto JM, Douglas Kinghorn A, ʺPotential cancer chemopreventive agents from Arbutus unedoʺ, Nat. Prod. Res. 2006; 20:327-334.
[32]: Prasad KN, Xie H, Hao J, Yang B, Qiu S, Wei X, Chen F, Jiang Y, ʺAntioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peelʺ, Food Chem. 2010; 118:62-66.
[33]: Gülçin İ, Bursal E, Şehitoğlu HM, Bilsel M, Gören AC, ʺPolyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurumʺ, Food Chem. Toxicol. 2010; 48:2227-2238.
[34]: Priyadharshini SD, Sujatha V, ʺAntioxidant assessment for various solvent fractions of Cassia fistula Linn. flowersʺ, Int. J. PharmTech Res. 2012; 4(1):510-517.
[35]: Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T, ʺPhenolics as potential antioxidant therapeutic agents: mechanism and actionsʺ, Mutat. Res. 2005; 579:200-213.
[36]: Kosanic M, Rankovic B, Dasic M, ʺAntioxidant and antimicrobial properties of mushroomsʺ, Bulg. J. Agric. Sci. 2013; 19(5):1042-1048.
[37]: Mira L, Fernandez MT, Santos M, Rocha R, Florencio MH, Jennings KR, ʺInteractions of flavonoids with iron and copper ions: a mechanism for their antioxidant activityʺ, Free Radic. Res. 2002; 36:1199-208.
[38]: Bajpa VK, Sharma A, Kang SC, Baek KH, ʺAntioxidant, lipid peroxidation inhibition and free radical scavenging efficacy of a diterpenoid compound sugiol isolated from Metasequoia glyptostroboidesʺ, Asian Pac. J. Trop. Med. 2014; 7: 9-15.
[39]: Lizcano ALJ, Viloria-Bernal M, Vicente F, Berrueta LA, Gallo B, Martınez-Ca~namero M, Ruiz-Larrea MB, Ruiz-Sanz JI, ʺLipid oxidation inhibitory effects and phenolic composition of aqueous extracts from medicinal plants of Colombianʺ, Int J Mol Sci. 2012; 13:5454-5467.
[40]: Saleh MR, Hasan MH, Adam A, ʺPhenolic compound and antioxidant levels of Prismatomeris rajnglabraʺ, J . Pharmacogn. Phytochem. 2015; 3:5-11.
[41]: Moyo B, Oyedemi S, Masika PJ, Muchenje V, ʺPolyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/ sunflower seed cakeʺ, Meat Sci. 2012; 91:441-447.
[42]: Salcedo CL, Frías MA, Cutro AC, Nazareno MA, Disalvo EA, ʺAntiradical activity of gallic acid included in lipid interphasesʺ, BBA-Biomembranes, 2014; 1838: 2656-2661.
[43]: Djeridane A, Yousfi M, Brunel JM, Stocker P, ʺIsolation and characterization of a new steroid derivative as a powerful antioxidant from Cleome arabica in screening the in vitro antioxidant capacity of 18 Algerian medicinal plantsʺ, Food Chem.Toxicol. 2010; 48:2599-606.
[44]: Varghese CP, Ambrose C, Jin SC, Lim YJ, Keisaban T, ʺAntioxidant and anti-inflammatory activity of Eurycoma longifolia Jack, a traditional medicinal plant in Malaysiaʺ, Int. J. Pharm. Sci. Nano. 2013; 5:1875-1878.
[45]: Yang HL, Chen SC, Chang NW, Chang JM, Lee ML, Tsai PC, Fu HH, Kao WW, Chiang HC, Wang HH, Hseu YC, ʺProtection from oxidative damage using Bidens pilosa extracts in normal human erythrocytesʺ, Food Chem. Toxicol. 2006; 44:1513-21.
[46]: Yang HL, Korivi M, Lin MK, Chang HC-W, Wu C-R, Lee M-S, Chen WT-L, Hseu Y-H, ʺAntihemolytic and antioxidant properties of pearl powder against 2,2′-azobis(2-amidinopropane) dihydrochloride-induced hemolysis and oxidative damage to erythrocyte membrane lipids and proteinsʺ, J. Food drug anal. 2017; 25:898-907.
[47]: Yu J, Ahmednaand M, Goktepe I, ʺEffects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolicsʺ, Food Chem. 2005; 90:199-206.
[48]: Dai F, Miao Q, Zhou B, Yang L, Liu ZL, ʺProtective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cellsʺ, Life Sci. 2006; 78:2488-93.
[49]: Chaudhuri S, Banerjee A, Basu K, Sengupta B, Sengupta PK, ʺInteraction of flavonoids with red blood cell membrane lipids and proteins: antioxidant and antihemolytic effectsʺ, Int. J. Biol. Macromol. 2007; 41(1):42-48.
[50]: De Freitas MV, Rita de Cassia MN, da Costa Huss JC, de Souza TMT, Costa JO, Firmino CB, Penha-Silva N, ʺInfluence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytesʺ, Toxicol. In Vitro, 2008; 22: 219-224.
[51]: Alvarez-Suarez JM, Giampieri F, Gonzalez-Paramas AM, Damiani E, Astolfi P, Martinez-Sanchez G, Bompadre S, Quiles JL, Santos-Buelga C, Battino M, ʺPhenolics from monofloral honeys protect human erythrocyte membranes against oxidative damageʺ. Food Chem Toxicol. 2012; 50: 1508-16.
[52]: Henneberg R, Otuki FM, Furman AEF, Hermann P, Nascimento AG, Leonart MSS, ʺProtective effect of flavonoids against reactive oxygen species production in sickle cell anemia patients treated with hydroxyureaʺ, Rev. Bras. Hematol. Hemoter. 2013; 3:52-5.
Statistics
12 Views | 13 Downloads
How to Cite
1.
Laouicha S, Senator A, Kherbache A, Bouriche H. Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts. JDDT [Internet]. 15Jun.2020 [cited 2Jul.2020];10(3-s):159-68. Available from: http://jddtonline.info/index.php/jddt/article/view/4182