Vaccine Preperative Trial for Leptospirosis and their Pathological, Immunological Study by Serum Electrophoresis

  • VISHNU KIRAN MANAM PG and Research Department of Microbiology, Jaya College of Arts & Science, University of Madras, Chennai, Tamilnadu, India - 602024
  • G Sumathi Leptospirosis Research Cell, Institute of Microbiology, Madras Medical College, Chennai 600003.Tamilnadu, India


Leptospirosis is a fatal infectious disease caused by different serovars of Leptospira spirochetes affecting humans and animals. In the present study, the trials of the whole-cell killed formalin treated monovalent vaccine using Leptospira icterohaemorrhagiae and trivalent vaccine using Leptospira icterohaemorrhagiae, Leptospira louisiana, and Leptospira hebdomadis were studied. The serum electrophoresis studies were done after administration of the vaccine into the experimental albino mice along with the booster dose of the vaccinated serum by densitometric readings. Similarly, the pathological observations were made by dissecting the virulent mice, vaccinated mice, and comparing them with the control mice. The MAT titre was also studied after the booster dose administration of the vaccinated serum. The monovalent and trivalent whole-cell killed formalin treated vaccines shows significant raise in the total proteins, albumin, globulin, α 1 globulin, α 2 globulin, β globulin and γ globulins of the serum as well as increase in significant levels in the antibody levels after the administration of the booster dose at an interval of 14 days.

Keywords: Leptospira, Whole-cell killed formalin treated vaccine, Immunological study, Pathological study, Serum electrophoresis.

Keywords: Leptospira, Whole-cell killed formalin treated vaccine, Immunological study, Serum electrophoresis


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Author Biographies

VISHNU KIRAN MANAM, PG and Research Department of Microbiology, Jaya College of Arts & Science, University of Madras, Chennai, Tamilnadu, India - 602024

PG and Research Department of Microbiology, Jaya College of Arts & Science, University of Madras, Chennai, Tamilnadu, India - 602024

G Sumathi, Leptospirosis Research Cell, Institute of Microbiology, Madras Medical College, Chennai 600003.Tamilnadu, India

Leptospirosis Research Cell, Institute of Microbiology, Madras Medical College, Chennai 600003.Tamilnadu, India


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MANAM VK, Sumathi G. Vaccine Preperative Trial for Leptospirosis and their Pathological, Immunological Study by Serum Electrophoresis. JDDT [Internet]. 15Jun.2020 [cited 17Jan.2021];10(3-s):116-27. Available from: