Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica

  • Karima Loucif Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Hassiba Benabdallah Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Fatima Benchikh Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Soulaf Mehlous Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Chawki Ben Souici Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria
  • Smain Amira Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Abstract

Phytotherapy has known a great evolution all the world and some medicinal plants are important remedies of some diseases. Ammoides atlantica is one of the medicinal plants used in folk medicine. This study aims to estimate the total phenolics and flavonoids contents then to investigate both in vitro antioxidant activity models of aqueous extract (AqE) from Ammoides atlantica. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 Method. The in vitro antioxidant property was assessed by DPPH-scavenging radicals and lipid peroxidation assays. The results revealed that Ammoides atlantica aqueous extract presented a high total phenolic and flavonoid contents with values of 85.56±4.71 µg GAE (gallic acid equivalent)/mg and 40.55±4.09 µg QE (quercitin equivalent)/mg dry extract, respectively. This extract shows a good DPPH radical scavenging and β-carotene bleaching activities with an IC50 of 107.48±5.9 µg/mL and 130.17±5.52 µg/mL, respectively. This study indicates that the aqueous extract from Ammoides atlantica has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs.


Keywords: Ammoides atlantica, aqueous extract, antioxidant activity, phenolic compounds.

Keywords: Ammoides atlantica, aqueous extract, antioxidant activity, phenolic compounds

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Author Biographies

Karima Loucif, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Hassiba Benabdallah, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Fatima Benchikh, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Soulaf Mehlous, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Chawki Ben Souici, Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria

Biotechnology Research Center (CRBt), UV 03 BP E73, Nouvelle Ville Ali Mendjli, Constantine, Algeria

Smain Amira, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

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Loucif K, Benabdallah H, Benchikh F, Mehlous S, Souici CB, Amira S. Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica. JDDT [Internet]. 30Jun.2020 [cited 2Jul.2020];10(3-s):196-8. Available from: http://jddtonline.info/index.php/jddt/article/view/4151