In-Vitro Antibacterial Activity of Endophytic Fungi from Peganum harmala (Laghouat, Algeria)

  • Yasmina Ouzid Faculty of sciences, Department of Biology, M’hamed Bougara University, Boumerdes, 35000, Algeria
  • Siliya Karaoui Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.
  • Noria Smail Saadoun Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.
  • Karim Houali Laboratory of analytic biochemistry and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Abstract

Medicinal plants are an inexhaustible source of molecules. They are colonized by mycoendophytes, fungi living in their tissues without apparent symptoms. These fungi can provide secondary metabolites with biological activities. It is with this in mind that we are interested in a spontaneous plant from the dayas region (Laghouat, Algeria): Peganum harmala or Harmel, a toxic medicinal plant belonging to the family Zygophyllaceae. Our study consists in highlighting the antibacterial activity of four kinds of mycoendophytes: Cladosporium, Alternaria, Aspergillus and Penicillium isolated from the leaves of this plant. The antibacterial activity is evaluated by the technique of the double disk diffusion on agar with respect to some Gram-positive bacterial strains. We have adopted two protocols for this purpose. For the first, the mycelia of all the mushrooms are deposited in the same petri dish. For the second, a single disc of the mycelium of a single species is deposited per box. The results obtained show a difference in the sensitivity of the bacterial strains to the bioactive substances of the mycoendophytes studied. The Alternaria genus showed the most significant activity. ANOVA performed between the mean diameters of the mycoendophyte inhibition zones and the antibiotic test disc: Chloramphenicol showed a highly significant difference between these two measurements. The Newman-Keuls test revealed a difference in the susceptibility of bacterial strains to the secondary metabolites of fungus mycoendophytes of Peganum harmala according to the two protocols used. The antibacterial effect is related to interactions between endophytic fungi and their host plant.

Keywords: mycoendophytes, antibacterial activity, Peganum harmala, Laghouat (Algeria), endophytic fungi

Downloads

Download data is not yet available.

Author Biographies

Yasmina Ouzid, Faculty of sciences, Department of Biology, M’hamed Bougara University, Boumerdes, 35000, Algeria

Faculty of sciences, Department of Biology, M’hamed Bougara University, Boumerdes, 35000, Algeria

Siliya Karaoui, Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.

Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.

Noria Smail Saadoun, Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.

Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria.

Karim Houali, Laboratory of analytic biochemistry and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Laboratory of analytic biochemistry and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

References

Akbary P, Fereidouni MS and Akhlaghi M. In vitro antibacterial activity of Peganum harmala (L) extract to some fish pathogenic bacteria. Iranian Journal of Aquatic Animal Health, 2014; 1(1): 7-16.
Arivudainambi US, Anantha Koteswararao E, Kotamraju S, Karunakaran C and Aajendran, A. Antibacterial effect of an extract of the endophytic fungus Alternaria alternata and its cytotoxic activity on MCF-7 and MDA MB-231 tumour cell lines. Biological Lett,2014; 51(1): 7-17.
Arnold AE, Maynard Z and Gilbert GS. Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycological Research, 2001; 105 (12): 1502-1520.
Arnold AE, Mejía LC, Kyllo D, Rojas EI, Maynard Z, Robbins N and Herre EA. Fungal endophytes limit pathogen damage in a tropical tree. The National Academy of Sciences, 2003; 100(26): 15649-15654.
Chomcheon P, Wiyakrutta S, Sriubolmas N, Ngamrojanavanich N, Isarangkul D and Kittakoop P. 3-Nitropropionic acid (3-NPA), a potent antimycobacterial agent from endophytic fungi: is 3-NPA in some plants produced by endophytes? Journal of Natural Products, 2005; 68(7):1103-1105.
Darabpour E, Bavi AP, Motamedi H and Nejad SMS. Antibacterial activity of different parts of Peganum harmala L. Growing in Iran against Multi-Drug Resistant Bacteria. EXCLI Journal, 2011; 25(10): 252-263.
Devaraju R and Satish S. Endophytic Mycoflora of Mirabilis jalapa L. and Studies on Antimicrobial Activity of its Endophytic Fusarium sp. Asian Journal of Experimental Biological Sciences, 2011 ; 2(1): 75-79.
Ellouz, O. Diversité des champignons endophytes mycorhiziens et de classe II chez le pois chiche, et influence du génotype de la plante. Thèse de Doctorat en Sciences, Université de Montréal, Canada, 2011 ; 132 pp.
Gimenez C, Cabrera R, Reina M and González-Coloma A. Bentham Science Publishers Ltd. Fungal Endophytes and their Role in Plant Protection.Current Organic Chemistry, 2007; 11(8):707-720.
Hellwig V, Grothe T, Mayer-Bartschmid A, Endermann R, Geschke FU, Henkel T and Stadler M. Altersetin, a new antibiotic from cultures of endophytic Alternaria spp. Taxonomy, fermentation, isolation, structure elucidation and biological activities. Journal of Antibiotics, 2002; 55: 881-892.
Javadian F, Saeidi S and Jahani S.Antimicrobial Activity of Peganum harmala and Heracleum persicum Against Acinetobacter baumannii. International Journal of Infection, 2016; 3(1): e33554.
Kaul S, Gupta S, Ahmed M and Dhar MK. Endophytic fungi from medicinal plants: a treasure hunts for bioactive metabolites. Phytochemistry Reviews, 2012; 11(4): 487-505.
Mandeel Q, Al-Laith A and Mohsen L . Survey of fusarium species in an arid environment of Bahrain. V. Antimicrobial activity of some local and International fusarium species. Pharmaceutical Biology, 1998; 37 (3): 181-187.
Nakamura CV, Ueda-Nakamura T, Bando E, Negrão Melo AF, Cortez DAG and Dias Filho BP. Antibacterial Activity of Ocimum gratissimum L. Essential Oil. Memorios do Instituto Oswaldo Cruz, 1999; 94(5): 675-678.
Niroumand MC, Farzaei MH and Amin G. Medicinal properties of Peganum harmala L. in traditional Iranian medicine and modern phytotherapy: a review. Journal of Traditional Chinese Medicine, 2015; 35(1): 104-109.
Schulz B, Boyle C, Draeger S, Rommert AK and Krohn K. Endophytic fungi: a source of novel biologically activesecondary metabolites. The British Mycological Society, Mycological Research, 2002; 106 (9): 996-1004.
Schulz B, Boyle C and Sieber TN. What are Endophytes?, Microbial Root Endophytes, Soil Biology, 2006; 9(1): 1-13.
Strobel G and Daisy B. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews, 2002; 67(4): 491-502.
Tabuc C. Flore fongique de différents substrats et conditions optimales de production des mycotoxines. Thèse de Doctorat en Sciences. INPT de Toulouse et de l’Université de Bucarest, France, 2007 ; 190 pp.
Valgas C, Machado de Souza S, Smânia EFA and Smânia JA. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology, 2007; 38(2): 25-35.
Zhao J, Zhou L, Wang J, Shan T, Zhong L, Liu X and Gao X. Endophytic fungi for producing bioactive compounds originally from their host plants. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology A, 2010; 10: 567-576.
Statistics
6 Views | 4 Downloads
How to Cite
1.
Ouzid Y, Karaoui S, Saadoun N, Houali K. In-Vitro Antibacterial Activity of Endophytic Fungi from Peganum harmala (Laghouat, Algeria). JDDT [Internet]. 15Jun.2020 [cited 2Jul.2020];10(3-s):47-1. Available from: http://jddtonline.info/index.php/jddt/article/view/4149