Antibacterial and Antifungal Effects of Carica papaya and Cucurbita specie Seed Extracts on Escherichia coli and Aspergillus flavus

  • Ogechukwu Calista Dozie-Nwakile Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
  • Calista Dozie Nwakile Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
  • Ngozi Felicia Onyemelukwe Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
  • Seto Aladenika Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
  • Adaobi Chisom Uchenna Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
  • Emmanuel Chibuzor Ugwu Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Abstract

Objective: In this work, we studied the antifungal and anti-bacterial properties of seeds of Carica papaya and Curcubita specie using selected bacteria and fungi.


Methods: Modified cold extraction method with ethanol and n-hexane was conducted. Antimicrobial properties of the extracts were done using agar block dilution for fungi and agar diffusion method for the bacteria. Measurement of the mean growth rate (MGR) for the fungi isolate and the inhibition zone diameter (IZD) for the bacteria were used as parameters.


Results: Significant antifungal property was observed in ethanolic extract of Carica papaya at a concentration of 6% at four days of its exposure, while n-hexane extract of Carica papaya and ethanolic extract of Curcubita specie show fungistatic action. Ethanolic extract of Carica papaya at 6% concentration showed more antifungal property than the control drug. Antibacterial action for all the test extracts was poor, with the control drug showing more significant action than the extracts. There was a statistical significance difference between the ethanolic extract of Carica papaya and Curcubita specie (p< 0.05).


Conclusion: This is an indication that ethanolic extract of Carica papaya can be used in the treatment of some of the fungal infection caused by Aspergillus flavus likewise n-hexane extracts.


Keywords: Antifungal, Anti-bacterial, Carica papaya, Curcubita specie, ethanolic extract, n-hexane extract.

Keywords: Antifungal, Anti-bacterial, Carica papaya, Curcubita specie, ethanolic extract, n-hexane extract

Downloads

Download data is not yet available.

Author Biographies

Ogechukwu Calista Dozie-Nwakile, Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Calista Dozie Nwakile, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Ngozi Felicia Onyemelukwe, Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Seto Aladenika, Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Adaobi Chisom Uchenna, Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Emmanuel Chibuzor Ugwu, Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

References

1. Jahan F, Lawrence R, Kumar V, Junaid M. Evolution of antimicrobial activities of plant extracts on antibiotic susceptible and resistant Staphylococcus aureus strains. JOCPR 2011; 3(4):777-789.
2. Akinyemi KO, Oluwa K, Omomigbehim EO. Antimicrobial activities of crude extract of the medicinal plants used in Southwest Nigeria folk medicine in some food borne pathogen. African journal of traditional chemistry 2006; 3(4):13-22.
3. Farnsworth NR, The role of Ethan I pharmacology in drug development. Bioactive components from plants.Ciba foundation symposium 154. John Wiley and sons. Baffin lane Duchester: 1990, 2-21.
4. Jaiswal P, Kumar P, Singh VK, Singh DK. Review article: Carica papaya Linn, a potential source of various health products. J pharm Res 2010; 3(5):998-1003.
5. Arumuganathan K, Earle ED,. Nuclear DNA, a content of some important plant species. Plant mol biol rep 1991; 9: 208-218.
6. Owoyele BV, Adebukola OM, Funmilayo A, Soladoye AO. Antiinflammatory activities of ethanolic extract of Carica papaya leaves. Inflammopharmacol 2008; 16:168-173.
7. Aravind G, Debjit B, Duraivel S, Harish G. Traditional and medicinal use of Caricapapaya. J Med Plant studies 2013; 1(1): 7-15.
8. Yogiraj V, Goyal PK, Chauhan CS, Goyal A, Vyas B. Carica papaya Linn: An overview. Int J Herbal Medicine 2014; 2(5): 1-8.
9. Jigna P, Nehal K, Sumitra C. Evaluation of antimicrobial and photochemical analysis of Bauhinia variegate Linn. bark. Afri J Biomed res, 2006; 9(1):53-57.
10. Hasham FA, Wahba HE. .Isothiocyanate in myrosinase treated herb extract of Clemiedirysanthadecne and their antimicrobial activity. Phytother res 2000; 14(4):284-287.
11. Chanwitheesule A, Teerawutqulreg A, Rakariyatham N. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem, 2005; 92:491-497.
12. Kalia VC, Rashmi SL. Modified cold percolation method for extracting oil from oil seeds. J Sci Ind res India 2002; 61(8):630-634.
13. Chessbough, M. District laboratory practice in tropical countries. Cambridge university press, South Africa, 2nd edition: 2006, pp 1-434.
14. Mohit k, Faheem M, Singh S, Shalizad A, Bhargava AK.Antifungal activity of Carica papaya important food and drug plant.Asian J Plant sci res 2013; 3(1):83-86.
15. Saddiq AA. Antimicrobial and therapeutics effect of the pumpkin plant (Curcubita moschata) against some harmful microbial. Egypt J Exp. Biol 2010; 6(1):41-49.
16. Mello VJ, Homes MT, Lemos FO, Delfino JL, Andrade SP, Lopes MT, Salas CE. The gastric ulcer protective role of cysteine proteinase from Carica candamarcensis. Phytomedicine. 2008; 15:237-244.
17. Mali RG, Mehta AA. A review on antihelminthic plants. NPR 2008: 7(5):466-475.
18. Fokou E, Achum M, Tchouanguep M. Preliminary evaluation of five species of egusi seeds in Cameroon. AJFAND, 2014, 4(1):1-11.
19. Rajakaruna N, Harris C, Tower G. Antimicrobial activity of plants collected from serpentine out crop in Sri Lanka. Pharm Biol. 2002; 40(3):235-244.
20. Wang H, Ng T. Isolation of curcurmoschine, a novel antifungal peptide abundant in arginine, glutamate and glycine residue from black pumpkin seed. Plant sci 2003; 24:969-972.
21. Rahmani A, Aldebasi Y. Potential role of Carica papaya and their Active Constituents in the prevention and treatment of diseases. Int J Pharm Pharm Sci 2015; 8(1):11-15.
22. Stuart H. Bacteria. Essential microbiology. John Wiley and sons limited. Atrium southern gate, Duchester West Sussex, England: 2005, pp. 1-478.
23. Consolacion YR, Kathleen L. Sterols from Cucurbita maxima on study of flowers; PJS 2005; 34(2):83-87.
24. Neelamma G, Durai-Swamy B, Dhamodaran P. Phytochemical and Pharmacological overview of Cucurbita maxima and future perspective as potential phytotherapeutic agent. EJPMR 2016; 3(8):277-287.
25. Chukeuemeka NO, Anthonia AB. Antifungal effect of pawpaw and papain in post harvestCaricapapaya Linn. fruit rot. Afr. J. Agric. Res 2010: 5(12):1531-1535.
26. Amna AN. Antifungal and prophylactic activity of Pumpkin ( Curcubitamoschata) extract against A.flavus and Aflatoxin B1. Afr J Microbiol res 2012; 6(41):6941-6947.
Statistics
25 Views | 19 Downloads
How to Cite
1.
Dozie-Nwakile OC, Nwakile CD, Onyemelukwe NF, Aladenika S, Uchenna AC, Ugwu EC. Antibacterial and Antifungal Effects of Carica papaya and Cucurbita specie Seed Extracts on Escherichia coli and Aspergillus flavus. JDDT [Internet]. 25Jun.2020 [cited 2Jul.2020];10(3-s):189-95. Available from: http://jddtonline.info/index.php/jddt/article/view/4133