Antioxidant chlorophyll purification from maize leaves by liquid-to-liquid extraction method
Chlorophyll is the blood of plant possesses a medical-value for treating support of disease in human, and the paper focused on the purification of antioxidant chlorophyll extracting from maize leaves in Vietnam. Chlorophyll was extracted with 96% ethanol and segmented by different solvents, for example, n-hexane, 96% ethanol, and ethyl acetate, respectively. Ethanol fraction was running via the chromatography column of silica gel for collecting antioxidant purified chlorophyll. All fractions were analysed chlorophyll content, antioxidant activities (total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity) and run the thin layer chromatography for determining the chlorophyll purification degree and the Rf. The results showed that the purification of antioxidant chlorophyll from maize leaves was by using the liquid-liquid segment and the column run, for example, in turn, ethanol, n-hexane, ethanol, and the silica gel chromatography. The highest value of chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity was 0.563±0.003 µg chl equivalent/ml, 1.392±0.018 mg ascorbic acid equivalent/ml, 3.396±0,024 mg FeSO4/ml, and 76.34±0.81 (%), respectively, as the n-hexane/ethanol ratio of 1/1 (v/v). After the chromatography run, chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity corresponded to 0.217±0.002 µg chl equivalent/ml, 0.628±0.013 mg ascorbic acid equivalent/ml, 1.928±0.019 mg FeSO4/ml, and 77.19±0.58 (%), respectively.
Keywords: antioxidant, chlorophyll, maize, purification, liquid-to-liquid
2. Natsuki H. Sachi SK. Nobel prizes for research in plant science: Past, present and future. Reviews in Agricultural Science 2017; 5:83-99.
3. Luxsika N. Sakamon D. Naphaporn C. Effects of pretreatment and drying methods on molecular structure, functional properties and thermal stability of fibre powder exhibiting colour from Centella asiatica L. Int J Food Sci. 2016; 51(3):753-764.
4. Aris H. Su L. Ronald H. Michael KD. Chlorophyll extraction from microalgae: A review on the process engineering aspects. Int J Chem Eng. 2010; 1-11.
5. Ursula ML-M. Rosa MCB. Patricia S. Antioxidant activity of chlorophylls and their derivatives. Food Res Int. 2005; 38:885-891.
6. Levent İA. Chlorophyll: Structural properties, health benefits and its occurrence in virgin olive oils. Akademik Gıda. 2011; 9(2):26-32.
7. Benjamin GMD. Chlorophyll-Its therapeutic place in acute and suppurative disease: Preliminary report of clinical use and rationale. The American Journal of Surgery. 1940; 49(1):49-55.
8. Kateřina V. Ivana M. Jana J. Aleš D. Iva S. Jaroslav Z. Iva N. Jan R. Tomáš V. Roman S. Lucie M. Libor V. Chlorophyll-mediated changes in the redox status of pancreatic cancer cells are associated with its anticancer effects. Oxid Med Cell Longev. 2018; 1-11.
9. Hiscox J. Tsraelstam G. A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot. 1979; 57:1332-1334.
10. Aouicha E. Hanane EH. Hassan F. Anouar A. Brahim EB. Mohammed L. Phytochemical screening and evaluation of antioxidant and antibacterial activities of seeds and pods extracts of Calycotome villosa subsp. Intermedia. Journal of Applied Pharmaceutical Science. 2017; 7(04): 192-198.
11. Vu NB. Dang XC. Phan TKV. Effects of extraction conditions over the phlorotannin content and antioxidant activity of extract from brown algae Sargassum serratum (Nguyen Huu Dai 2004). Free Radicals and Antioxidants. 2017; 7(1):115-122.
12. Dang XC. Vu NB. Tran TTV. Le NH. Effect of storage time on phlorotannin content and antioxidant activity of six Sargassum species from Nhatrang Bay, Vietnam. J Appl Phycol. 2016; 28(1):567-572.
13. Pierre N. Edith NF. Carl MFM. Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves. Food Sci Nutr. 2018; 1-11.
14. Ursula ML-M. Rosa MB. Patricia S. Antioxidant activity of chlorophylls and their derivatives. Food Res Int. 2005; 38:885-891.
15. Dang XC. Nguyen XH. Dinh HD. Le TMT. Nguyen VT. Hoang TH. Dang TTT. Dang XC, Tannins: Extraction from plants. In: Alfredo A, editor. Tannins - Structural properties, biological properties and current knowledge. IntechOpen. 2019; 1-20.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).