Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

  • Ahlem Karbab Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Kamel Mokhnache Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Lekhmici Arrar Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Abderahmane Baghiani Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Seddik Khennouf Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria
  • Noureddine Charef Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Abstract

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, flavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants.


Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

Keywords: Medicinal plant, phytochemical screening, free radical scavenging

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Author Biographies

Ahlem Karbab, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Kamel Mokhnache, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Lekhmici Arrar, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Abderahmane Baghiani, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Seddik Khennouf, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Noureddine Charef, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif-1, 19000 Algeria

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Karbab A, Mokhnache K, Arrar L, Baghiani A, Khennouf S, Charef N. Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots. JDDT [Internet]. 15May2020 [cited 1Jun.2020];10(3):133-6. Available from: http://jddtonline.info/index.php/jddt/article/view/4100