Extraction, Phytochemical Screening and Anti-Inflammatory Activity of Hydro-Ethanolic Extract of Roots of Dactylorhiza hatagirea
The present work showed phytochemical screening, anti-inflammatory activities and sub-acute toxicity of hydro-ethanolic extract of roots of Dactylorhiza hatagirea (D. hatagirea) and rhizomes of Curcuma angustifolia (C. angustifolia). The anti-inflammatory activity was evaluated by Carrageenan-Induced Rat Paw Edema method. Acute toxicity of the extract (2000 mg/kg) was examined in Swiss albino mice for 14 days. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of roots of Dactylorhiza hatagirea and rhizomes of Curcuma angustifolia extract was (0.675, 0.456mg/100mg), followed by flavonoids (0.832, 1.091mg/100mg) respectively. Hydro-ethanolic extract up to 2000 mg/kg did not produce any toxic effects. The hydro-ethanolic extract of Dactylorhiza hatagirea and Curcuma angustifolia (100 and 200 mg/kg) inhibited the inflammation induced by carrageenan in rats in a dose dependent manner. After anti-inflammatory activity tablet formulation was prepared of the extract and evaluated as per IP. The hydro-ethanolic extract of Dactylorhiza hatagirea and Curcuma angustifolia possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.
Keywords: Sub-acute toxicity, anti-inflammatory effect, Dactylorhiza hatagirea, Curcuma angustifolia, Herbal tablet formulation
2. Abdelmagid SM, Barbe MF, Safadi FF, Role of inflammation in the aging bones, Life Sci., 2015; 123:25–34.
3. McCarty MF, Interleukin-6 as a central mediator of cardiovascular risk associated with chronic inflammation, smoking, diabetes, and visceral obesity: down-regulation with essential fatty acids, ethanol and pentoxifylline, Med Hypotheses, 1999; 52:465–477.
4. Azeem AK, Dilip C, Prasanth SS, Shahima VJH, Sajeev K, Naseera C, Anti-inflammatory activity of the glandular extracts of Thunnus alalunga, Asia Pac J Med, 2010; 3:4–412.
5. Medzhitov R, Inflammation 2010: new adventures of an old flame, Cell, 2010; 140:771–776.
6. Kumar V, Bhat ZA, Kumar D, Bohra P, Sheela S, In-vitro anti-inflammatory activity of leaf extracts of Basella alba linn. Var. alba, Int J Drug Dev Res, 2011; 3:124–127.
7. Vane JR, Botting RM, Anti-inflammatory drugs and their mechanism of action, Inflamm Res, 1998;47:78–87.
8. Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag NB, Iputo JE, An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation, J Med Plants Res, 2010; 4:789–795.
9. Gorzalczany S, Lopez P, Acevedo C, Ferraro G, Anti-inflammatory effect of Lithrea molleoides extracts and isolated active compounds, J Ethnopharmacol, 2011;133:994–998.
10. Birkedal-Hansen H, Role of cytokines and inflammatory mediators in tissue destruction, J Periodont Res, 1993; 28:500–510.
11. Hernandez M, Dutzan N, Garcıa-Sesnich J, Abusleme L, Dezerega A, Silva N, Gonzalez FE, Vernal R, Sorsa T, Gamonal J, Host-pathogen interactions in progressive chronic periodontitis, J Dent Res, 2011; 90:1164–1170.
12. Griffin MR, Epidemiology of nonsteroidal anti-inflammatory drug-associated gastrointestinal injury, Am J Med, 1998; 104:23–29.
13. Bhattarai NK, Some endangered medicinal plants of Nepal. In: Handa SS, Kaul MK (eds). Supplement to Cultivation and Aphrodisiac Activity of Dactylorhiza hatagirea Utilization of Medicinal Plants. Jammu: Regional Research Laboratory, 1996; 676–7.
14. Pereira J, The contribution of Jonathan Pereira to plant taxonomy, key volume bulletin, 2011; 66:1804-1853.
15. Bhavna D, Khatri J, Gentella asiatica: The elixir of life. Inter J Pharmaceutical Res 2011; 2(2):431-438.
16. Mukherjee PK, Quality Control of Herbal Drugs”, 2nd Edition, Business Horizons, 2007; 2-14.
17. Khandelwal KR, Ed. Practical Pharmacognosy Technique and Experiments, 23rd Edn, 2005; 15, 29, 149, 56.
18. Kokate CK. Ed. Practical Pharmacognosy, 4th Edn., Vallabh Prakashan, 1994; 112,120.
19. Olufunmis O, Olajuyigbe, O. and Afolayan, Anthony J, phenolic Content and antioxidant property of the bark extract of Ziziphus mucronata wild. Subsp. Mucronata wild, BMC, Complementary and alternative medicine, 2011; 11:130.
20. Guideline Document on Acute oral Toxicity Testing, Series on Testing and Assessment No. 423. Paris: Organization for Economic Co-Operation and Development, OECD Environment, Health and Safety Publications; 1996. Available from: http//www.oecd.org/ehs.
21. Jonsson M, Jestoi M, Nathanail AV, Kokkonen UM, Anttila M, Koivisto P, Peltonen K, Application of OECD Guideline 423 in assessing the acute oral toxicity of moniliformin, Food and chemical toxicology, 2013; 53:27-32.
22. Winter CA, Risley EA, Nuss GW, Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs, Proc Soc Exp Biol Med, 1962; 111:544–547.
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