In Vivo Antioxidant Potential of Protein Hydrolysates of some Cucurbitaceae Seed
Nowadays, dietary proteins play a very crucial role against oxidation, which is a fundamental process in the occurrence of many diseases. The aim of this study was to hydrolyze globulin fractions from C. moschata (CMH), C. lanatus (CLH) and L. siceraria (LSH), and to evaluate their in vivo antioxidant potential. The in vivo antioxidant potential was conducted using in vivo catalase and lipid peroxidation (LPO) assay methods. The total protein content was also estimated using Bio-Rad protein assay and bovine serum albumin methods. For in vivo study, ethanol was induced into test animals for toxicity and oxidative damage for 15 days. The catalase and LOP of serum / liver homogenate were determined. The results showed that globulin hydrolysates decrease the MDA level and increase the catalase level in normal mice. Based on these findings, this study provides information about in vivo antioxidant activities of C. moschata, C. lanatus and L. siceraria hydrolysates. Globulin hydrolysates of L. siceraria showed effective antioxidant properties in vivo models, whereas globulin hydrolysates of C. moschata and C. lanatus exhibited remarkable antioxidant properties, as well. Thus, the results suggest that globulin hydrolysates might be used as a novel source in reducing the risk of oxidative stressed diseases and development of functional foods.
Keywords: cucurbitaceae, globulin, antioxidant, trypsin
2. Henning SM, Yang J, Shao P, Lee RP, Huang J, Ly A, Hsu M, Lu QY, Thames G, Heber D, Li Z. Health benefit of vegetable/fruit juice-based diet: Role of microbiome. Scientific reports. 2017 May 19; 7(1):1-9.
3. Caili FU, Huan S, Quanhong LI. A review on pharmacological activities and utilization technologies of pumpkin. Plant foods for human nutrition. 2006 Jun 1; 61(2):70-7.
4. Hasler CM. Functional foods: benefits, concerns and challenges—a position paper from the American Council on Science and Health. The Journal of nutrition. 2002 Dec 1; 132(12):3772-81.
5. Hernández-Ledesma B, Hsieh CC, editors. Bioactive food peptides in health and disease. BoD–Books on Demand; 2013 Jan 30.
6. Patel S, Rauf A. Edible seeds from Cucurbitaceae family as potential functional foods: Immense promises, few concerns. Biomedicine & Pharmacotherapy. 2017 Jul 1; 91:330-7
7. Kinsella JE. Protein structure and functional properties: Emulsification and flavor binding effects. In ACS Symposium series-American Chemical Society (USA). 1982.
8. Singh Gill N, Sood S, Muthuraman A, Bali M, Dev Sharma P. Evaluation of antioxidant and anti-ulcerative potential of Citrullus lanatus seed extract in rats. Latin American journal of pharmacy. 2011; 30.
9. Thaiphanit S, Anprung P. Physicochemical and emulsion properties of edible protein concentrate from coconut (Cocos nucifera L.) processing by-products and the influence of heat treatment. Food Hydrocolloids. 2016 Jan 1; 52:756-65.
10. Ozuna C, León-Galván M. Cucurbitaceae seed protein hydrolysates as a potential source of bioactive peptides with functional properties. BioMed Research International. 2017; 2017.
11. Clare DA, Swaisgood HE. Bioactive milk peptides: a prospectus. Journal of dairy science. 2000 Jun 1; 83(6):1187-95.
12. Saadi S, Saari N, Anwar F, Hamid AA, Ghazali HM. Recent advances in food biopeptides: Production, biological functionalities and therapeutic applications. Biotechnology advances. 2015 Jan 1; 33(1):80-116.
13. Dash P, Ghosh G. Amino acid composition, antioxidant and functional properties of protein hydrolysates from Cucurbitaceae seeds. Journal of food science and technology. 2017 Dec 1; 54(13):4162-72.
14. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 1976 May 7; 72(1-2):248-54.
15. Nazeer RA, Kumar NS, Ganesh RJ. In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate. Peptides. 2012 Jun 1; 35(2):261-8.
16. Onoja SO, Omeh YN, Ezeja MI, Chukwu MN. Evaluation of the in vitro and in vivo antioxidant potentials of Aframomum melegueta methanolic seed extract. Journal of Tropical Medicine. 2014; 1-6.
17. Farbiszewski R, Chwiecko M, Holownia A, Pawlowska D. The decrease of superoxide dismutase activity and depletion of sulfhydryl compounds in ethanol-induced liver injury. Drug and alcohol dependence. 1991 Oct 1; 28(3):291-4.
18. Schisler NJ, Singh SM. Effect of ethanol in vivo on enzymes which detoxify oxygen free radicals. Free Radical Biology and Medicine. 1989 Jan 1; 7(2):117-23.
19. Escarabajal D, Miquel M, Aragon CM. A psychopharmacological study of the relationship between brain catalase activity and ethanol-induced locomotor activity in mice. Journal of studies on alcohol. 2000 Jul; 61(4):493-8.
20. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal. 2013 Apr 1; 21(2):143-52.
21. Tang X, Wu Q, Le G, Shi Y. Effects of heat treatment on structural modification and in vivo antioxidant capacity of soy protein. Nutrition. 2012 Nov 1; 28(11-12):1180-5.
22. Oyenihi OR, Afolabi BA, Oyenihi AB, Ogunmokun OJ, Oguntibeju OO. Hepato-and neuro-protective effects of watermelon juice on acute ethanol-induced oxidative stress in rats. Toxicology reports. 2016 Jan 1; 3:288-94.
23. Li M, Carlson S, Kinzer JA, Perpall HJ. HPLC and LC-MS studies of hydroxylation of phenylalanine as an assay for hydroxyl radicals generated from Udenfriend’s reagent. Biochemical and biophysical research communications. 2003 Dec 12; 312(2):316-22.
24. Fan J, He J, Zhuang Y, Sun L. Purification and identification of antioxidant peptides from enzymatic hydrolysates of tilapia (Oreochromis niloticus) frame protein. Molecules. 2012 Nov; 17(11):12836-50.
25. Torres-Fuentes C, del Mar Contreras M, Recio I, Alaiz M, Vioque J. Identification and characterization of antioxidant peptides from chickpea protein hydrolysates. Food Chemistry. 2015 Aug 1; 180:194-202.
26. Guo H, Kouzuma Y, Yonekura M. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry. 2009 Mar 1; 113(1):238-45.
27. Sanogo R, Germano MP, D'Angelo V, Guglielmo M, De Pasquale R. Antihepatotoxic properties of Entada africana (Mimosaceae). Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 1998; 12(S1):S157-9.
28. Soliman AM, Abu-El-Zahab HS, Alswiai GA. Efficacy evaluation of the protein isolated from Peganum harmala seeds as an antioxidant in liver of rats. Asian Pacific journal of tropical medicine. 2013 Apr 13; 6(4):285-95.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).