Antihyperglycemic activity of total extract and fractions of Anogeissus leiocarpus

  • Enam Aku MOTTO Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515
  • Povi Lawson-Evi Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515
  • Yendube Kantati Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515
  • Kwashie Eklu-Gadegbeku Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515
  • Kodjo Aklikokou Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515
  • Messanvi Gbeassor Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Abstract

Anogeissus leiocarpus (Combretaceae) is a medicinal plant used by traditional practitioners to treat people living with diabetes mellitus in Togo. The objective of this work was to evaluate the hypoglycemic activity of the plant. The hydroalcoholic extract and fractions of A. leiocarpus roots was evaluated on hyperglycemic mice by oral glucose tolerance test (OGTT) and on normoglycemic mice. The effect of the total extract and fractions was also measured on the adsorption and absorption of glucose respectively in vitro and ex vivo. At 30 minutes after glucose overload, the total extract and all fractions (supernatant and pellet) significantly (p< 0.0001) reduced hyperglycemia compared to controls. However, this reduction in hyperglycemia was greater in mice treated with the supernatant fraction at 100 mg.kg-1 during 180 minutes (p < 0.01). In our conditions, the extract at the 500 mg.kg-1 of the total extract in normoglycemic mice did not significantly decrease (p>0.05) basal blood glucose levels compared to controls. In vitro, the extract and fractions of A. leiocarpus adsorbed glucose and inhibited glucose intestinal absorption ex vivo. The results of this study support the use of A. leiocarpus as an antidiabetic plant.


Keywords:  Anogeissus leiocarpus- fractions- antihyperglycemic- adsorption – absorption.

Keywords: Anogeissus leiocarpus, antihyperglycemic, adsorption

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Author Biographies

Enam Aku MOTTO, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Povi Lawson-Evi, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Yendube Kantati, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Kwashie Eklu-Gadegbeku, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Kodjo Aklikokou, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Messanvi Gbeassor, Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

Laboratory of Physiology / Pharmacology. Faculty of Sciences, University of Lome – TOGO. BP 1515

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MOTTO EA, Lawson-Evi P, Kantati Y, Eklu-Gadegbeku K, Aklikokou K, Gbeassor M. Antihyperglycemic activity of total extract and fractions of Anogeissus leiocarpus. JDDT [Internet]. 15May2020 [cited 1Jun.2020];10(3):107-13. Available from: http://jddtonline.info/index.php/jddt/article/view/4078