Acute Oral Toxicity Studies of the Crude Extract of Endophytic Fungi Isolated from Annona senegalensis Pers
Establishment of safety and toxicity profiles of metabolites of endophytic organisms from known medicinal plants are crucial in their pharmacological and biological applications. The aim of this study was to evaluate the oral acute toxicity (LD50) of crude extract of endophytic fungi isolated from Annona senegalensis Pers. The endophytic fungal metabolite was extracted with ethyl acetate. The LD50 was estimated following the method described by Lorke. Three dose levels (10, 100, and 1000 mg/kg) of the crude extract were administered to three mice each for the first phase using oral gavage needle in a single dose disposable syringe. The animals were observed for possible deaths or other side effects of the test substance in each group within 24 hours of the treatment. In the second phase, which was deduced from the first phase, eight mice were sub-divided into four groups of two mice each and they were treated with doses of 1200, 1600, 2900 and 5000 mg/kg orally. They were also observed within 24 hours and final LD50 value was determined. Results showed that the endophytic fungal extract exhibited no mortality or any histological defect in the liver tissues of the mice. More so, the immunological parameter tested showed significant increase in neutrophils and lymphocytes relative to the control in all the fungal isolates. Additionally, the LD50 for the crude metabolites was > 5000 mg/kg. This study has revealed that crude extract of endophytic fungi isolated from Annona senegalensis Pers did not show oral acute toxicity in mice. Further studies will evaluate long term-toxicity of the crude extract.
Keywords: Endophytes, LD50, Annona senegalensis, Metabolites, Fungi
2. Sheah Y G, Jin H C, Yin YT, Hip SY, Chun W H. Acute Oral Toxicity Study of Aquilaria Crassna and Α-Tocopherol In Mice. IJPSR, 2016; 7 (4):1456-1461.
3. Kale OE, Awodele O, Akindele AJ. Subacute and subchronic oral toxicity assessments of Acridocarpus smeathmannii (DC.) Guill. & Perr. root in Wistar rats, Toxicol. Rep.2019; 6:161–175.
4. Ahmad L, He Y, Hao J C, Semotiuk A, Liu QR, Mazari, P. Toxic pyrrolizidine alkaloids provide a warning sign to overuse of the ethnomedicine Arnebia benthamii, J. Ethnopharmacol. 2018; 210:88–94.
5. Kpemissia M, Metowogoa K, Melilaa M, Veerapurc V P, Negrub M., Taulescub M., Adrian-Valentin P, Suhasc DS, Puneethc TA,Vijayakumarc S, Eklu-Gadegbekua K, Aklikokoua K. Acute and subchronic oral toxicity assessments of Combretum micranthum (Combretaceae) in Wistar rats Toxicology Reports 2020; 7:162–168
6. Potchoo Y, Richard D, Sakie E, Guissou IP, Kini F, Yaro B. Comparative Phytochemical Content of Leaves Extract of two Annona Senegalensis Pers: The one from Togo and the other Originates from Burkina Faso. Journal of Biological Sciences 2008; 8(3):577-583.
7. Koto-te-Nyiwa N, Emmanuel LM., John LB, Ruphin DD, Colette MA, Pius TM. A mini-review of the Pharmacognosy and Phytochemisry of a Tropical Medicinal Plant. Annona Senegalensis Pers. Tropical Plant Research 2017; 4(1):168-175.
8. Gadaleta D , Vuković1 K., Toma1 C, Lavado1 GJ, Karmaus A.L, Mansouri K., Kleinstreuer NC, Benfenati E, Roncaglioni, A. SAR and QSAR modeling of a large collection of LD50 rat acute oral toxicity data Cheminform 2019; 11:58
9. OECD Test No. 425: acute oral toxicity: up-and-down procedure,OECD guidelines for the testing of chemicals, section 4, OECD Publishing, Paris. 2008.
10. Kennedy GL Jr, Graepel GJ. Acute toxicity in the rat following either oral or inhalation exposure. Toxicol Lett 1991; 56(3):317–326.
11. Garry S, Diasy B, Uvidello C. The Biological Promise of Microbial Endophyte and their Natural Products. Plant Pathology Journal 2005; 4(2):161-176.
12. Khaled A S, Waill A E, Ahmed MT, Ahmed AE, Tahany M Abdel-R, Ahmed I.E and Eman F A. Antiviral and Antioxidant Potential of Fungal Endophytes of Egyptian Medicinal Plants. Fermentation 2018; 4, 49.
13. Tan R, Zou W. Endophytes: A rich source of functional metabolites. Nat. Prod. Rep. 2001; 18:448–459.
14. Herrera H, Sanhueza T, Alžbˇeta Novotná, Trevor C C, Cesar A. Isolation and identification of endophytic bacteria from mycorrhizal tissues of terrestrial orchids from southern Chile. Diversity 2020; 12(55):1-12.
15. Lorke, D. “A new approach to practical acute toxicity testing,” Archives of Toxicology, 1983; 54(4):275–287,
16. Okezie MU, Eze P M, Okoye FBC, Ikegbunam MN, Ugwu MC, Esimone CO. Secondary Metabolites from an endophytic fungus of Vernonia amygdalina. African Journal of Pharmaceutical Research and Development. 2017; 9 (1):24-26.
17. Simth BJ, Black LL. Morphological, cultural and pathogenic variation among Colletotrihum species isolated from strawberry. Plant Diseases 1990; 74:69–67.
18. Sofowora, A. Medicinal Plants and Traditional Medicine in Africa, Spectrum Books Limited, Ibadan, Nigeria 1993 P 34-45.
19. Ochei J, Kolhatkar A. Medical laboratory science: theory and practice. Tata McGraw-Hill Publishing Company Limited, new Delhi.2007; PP 265-287.
20. Randox laboratory limited. Manual/RX Monza. 55 Diamond Road Crumlin, County Antrim United Kingdom.2014; PP. 1-10.
21. Cheesbrough M. District laboratory practice in tropical countries. Cambridge University Press, United kingdom. 2000; Pp 296 – 327.
22. Sarah CF, John SM, Jan MS, Sweta B. Toxicity Studies of Chanoclavine in Mice. Toxins 2019; 11(249):1-11
23. Abutaha N, AL-Zharani M, AL-Mekhlafi FA, AL-Tamimi J, Mashaly AMA, Wadaan M A. Acute and Subacute Toxicity of Ethyl Acetate Fraction of Cochliobolus spicifer (Nelson) isolated from Phoenix dactylifera(Linnaeus) on Balb/c Mice. Res. J. Biotech. 2017; 12(4) 2-6.
24. Samuel EO, Edifofon A., Omolola TF, Kevwe BE, Oluyemisi FK. Annona senegalensis Person (Annonoaceae): A review of its ethnomedicinal uses, biological activites and phytocompound. Journal of Pharmacognosy and Phytochemistry 2016; 5(2):211-219.
25. Xiao W, Wang X, Wang C, Wang M, Fei C, Zhang L, Xue F, Wang G, Zhang K. Acute and 30-day oral toxicity studies of a novel coccidiostat – ethanamizuril, Toxicology Research, 2019; 8(5):686–695
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