Antimicrobial and Hepatoprotective Potential of Pterospermum acerifollium leaves Extracts on Swiss albino mice

  • Deepa Varandani Dr. A.P.J. Abdul Kalam University, Indore, MP, India
  • Sharmishta Sekhar Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India
  • Suman Trivedi Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India
  • Megha Jha Pinnacle Biomedical Research Institute, Bhopal MP, India
  • Sourabh Jain Dr. A.P.J. Abdul Kalam University, Indore, MP, India

Abstract

The objective of present study to investigate the hepatoprotective activity of hydro-alcohollic extract leaves of Pterospermum acerifollium against antitubercular drug induced liver damage in swiss albino mice and also performs antimicrobial activity by disc diffusion assay. Successive extractions was performed with different organic solvents viz; hydroalcohollic by cold maceration. The extract was analysed as antioxidant activity as a content of Total phenolic content, Total flavanoid content, Reducing power assay and DPPH Scavenging assay. Antimicrobial activity of methanolic extract was estimated by Agar well diffusion method. Antitubercular drug induced is used as toxicants in hepatoprotective studies in acute condition was analysed by serum biochemical estimations by AST, ALT, ALP and Total Bilirubin. In-vivo Antioxidant activity was performed by LPO, GSH, SOD and Catalase. During the collection of tissue for biochemical estimation piece of tissue cut and transferred for Histopathological estimation. The levels were measured and it indicated that the extract had significant antioxidant activity however the results obtained were dose dependent the higher the dose (400 mg/kg) the better activity. The extract administered at dose 400 mg/kg showed better activity. The treatment with hydroalcohollic extract of Pterospermum acerifillium reduced the elevated levels of SGOT, SGPT, ALP, TB and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity.


Keywords: Succesive extraction, In-vivo, Serum biochemical, Cold maceration

Keywords: Succesive extraction, In-vivo, Serum biochemical, Cold maceration

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Author Biographies

Deepa Varandani, Dr. A.P.J. Abdul Kalam University, Indore, MP, India

Dr. A.P.J. Abdul Kalam University, Indore, MP, India

Sharmishta Sekhar, Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India

Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India

Suman Trivedi, Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India

Department of Microbiology, Govt. M.L.B. Girls P.G. (Autonomous) College, Bhopal MP, India

Megha Jha, Pinnacle Biomedical Research Institute, Bhopal MP, India

Pinnacle Biomedical Research Institute, Bhopal MP, India

Sourabh Jain, Dr. A.P.J. Abdul Kalam University, Indore, MP, India

Dr. A.P.J. Abdul Kalam University, Indore, MP, India

References

1. Lewis H.W& Elvin-Lewis M.P.H. Medical Botany: Plants Affecting Man’s Health. John Wiley and Sons, New York. 1977, 217-18.
2. Blois M S. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181:1199-1200.
3. Zhang, Donglin, et.al., Phenolics Compounds and Their Antioxidant Properties in Different Tissues of Carrots (Daucus carota L.), Food, Agriculture, & Environment, 2004; 2 (1):95-100.
4. Bauer AW, Kirby WM, Sherris JC. turck, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology. 1966; 45(4):493
5. Kharpate S, Vadnerkar G, Jain D, Jain S., hepatoprotective activity of the ethanol extract of the leaf of Ptrospermum acerifolium. Indian journal of pharmaceutical sciences, 2007; 69(6):850-52.
6. Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N.Türkiye Bitkileri Kırmızı Kitabı (Red Data Book of Turkish Plants) Barı_can Ofset, Ankara. Garibaldi RA, Drusin, RE, Ferebee, SH, Gregg MB 1972. Isoniazid associated hepatitis. Report of an outbreak. Am Rev Respir Dis 2000; 106:357-365.
7. Tietz, N.W., Burtis, C.A., Duncan, P., Ervin, K., Petitclerc, C.J., Rinker, A.D. A reference method for measurement of alkaline phosphatase activity in human serum. Clinical Chemistry 1983; 29:751-61.
8. Ohkawa H., Oniwa N., Yagi K., Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-358.
9. Sedlak J., Rudolf L., Estimation of total, protein-bound and non-protein bound sulphydryl groups in tissues with Ellman's reagent. Anal Biochem; 1968; 25:192-205.
10. Aebi H., Catalase. In: Mehtods in enzymatic analysis. Academic Press 1974; 2:674-84.
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Varandani D, Sekhar S, Trivedi S, Jha M, Jain S. Antimicrobial and Hepatoprotective Potential of Pterospermum acerifollium leaves Extracts on Swiss albino mice. JDDT [Internet]. 15May2020 [cited 1Jun.2020];10(3):170-4. Available from: http://jddtonline.info/index.php/jddt/article/view/4032