Assessment of histo-reparative effect of methanolic extract of Camellia sinensis in rat model of Lead-induced testicular damage

  • Dayo Rotimi Omotoso Department of Anatomy, Faculty of Basic Medical Sciences, Redeemer's University, Ede, Osun State, Nigeria
  • Adeniran Oluwadamilare Akinola Department of Physiology, College of Health Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria
  • Winners Prince Ehiemere Department of Anatomy, College of Health Sciences, Igbinedion University, Okada, Edo State, Nigeria

Abstract

Objective: To assess reparative effect of methanolic extract of Camellia sinensis on testicular histomorphology of male Wistar rats exposed to lead-induced testicular damage.  Methods: Twenty four male Wistar rats were grouped into four A–D (n = 6). These include normal control group A (distilled water 5 ml/kg), test control group B (lead acetate 30 mg/kg), treatment group C (lead acetate 30 mg/kg + MECS 5 mg/kg) and treatment group D (lead acetate 30 mg/kg + MECS 10 mg/kg). Administrations were through oral route for a period of 60 days and body weight of study animals was recorded during days 0, 20, 40 and 60 of study. Phytochemical analysis of extract was done using GCMS. At the end of sudy, testicular tissue weight of study animals was measured followed by histopathological study. Results: The extract significantly ameliorated body weight loss and exhibited histo-reparative effect on testicular histomorphology of study animals following lead exposure. Conclusion: Methanolic extract of Camellia sinensis exhibits reparative effect against toxic effect of tissue toxicant due to its antioxidant activity.


Keywords: Camellia sinensis, Lead toxicity, Testis histomorphology, Wistar rats

Keywords: Camellia sinensis, Lead toxicity, Testis histomorphology, Wistar rats

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Author Biographies

Dayo Rotimi Omotoso, Department of Anatomy, Faculty of Basic Medical Sciences, Redeemer's University, Ede, Osun State, Nigeria

Department of Anatomy, Faculty of Basic Medical Sciences, Redeemer’s University, Ede, Osun State, Nigeria

Adeniran Oluwadamilare Akinola, Department of Physiology, College of Health Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria

Department of Physiology, College of Health Sciences, University of Medical Sciences, Ondo City, Ondo State, Nigeria

Winners Prince Ehiemere, Department of Anatomy, College of Health Sciences, Igbinedion University, Okada, Edo State, Nigeria

Department of Anatomy, College of Health Sciences, Igbinedion University, Okada, Edo State, Nigeria

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Omotoso D, Akinola A, Ehiemere W. Assessment of histo-reparative effect of methanolic extract of Camellia sinensis in rat model of Lead-induced testicular damage. JDDT [Internet]. 15Aug.2020 [cited 23Apr.2021];10(4-s):46-2. Available from: http://jddtonline.info/index.php/jddt/article/view/4007