Ameliorative effect of hydroalcoholic extracts of Nigella sativa seed against CCl4-induced acute liver injury in rats

  • Ikenna Kingsley Uchendu Department of Medical Laboratory Science, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria


Objective:  The aim of this study is to investigate the ameliorative effect of hydroalcoholic extract of Nigella sativa (HANS) against CCl4-induced hepatotoxicity in albino rats. Methods: Twenty five (25) albino rats, with average weight (105±5g), were randomly grouped into five groups: A-E, of five (5) rats per group. Group A rats served as normal control, Group B (Negative Control) received intraperitoneal administration of carbon tetrachloride CCl₄ (0.4ml/kg, i.p.) only, Group C received CCl₄ and low dose HANS (400mg/kg, oral), Group D received CCl₄ and high dose HANS (800mg/kg, oral), and Group E (Positive control), received CCl₄ and Vitamin C (200mg/kg, oral), for 3 days. Hepatotoxicity was assessed by measuring serum levels of total bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) using standard methods.  Histopathological analysis of the liver was also carried out. Results: The extracts significantly stablized biochemical markers of hepatic injury, and preserved the histoarchitecture of the liver tissues against CCl4 damage. The protective effect was not dose-dependent, as low dose HANS (400mg/kg), showed better protection than the high dose HANS (800mg/kg). Conclusion: Hydroalcoholic extracts of Nigella sativa has antihepatotoxic effects.

Keywords: carbon tetrachloride, hepatotoxicity, medicinal plants, Nigella sativa, hydroalcoholic extract

Keywords: carbon tetrachloride, hepatotoxicity, medicinal plants, Nigella sativa


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Author Biography

Ikenna Kingsley Uchendu, Department of Medical Laboratory Science, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria

Department of Medical Laboratory Science, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria


1. Hosseini M, Mohammadpour T, Karami R, Rajaei Z, Sadeghnia HR, Soukhtanloo M. Effects of the hydro-alcoholic extract of Nigella sativa on scopolamine-induced spatial memory impairment in rats and its possible mechanism. Chinese Journal of Integrative Medicine. 2015; 21(6):438-444
2. Tavakkoli A, Ahmadi A, Razavi BM, Hosseinzadeh H. A review of miracle herb: Nigella sativa. Iran J Pharm Res. 2017; 16:2-23.
3. Amin B, Hosseinzadeh H. Black cumin (Nigella sativa) and its active constituent, thymoquinone: an overview on the analgesic and anti-inflammatory effects. Planta Medical. 2015; 82:8-16
4. Uchendu IK, Nnedu EB, Orji OC. Combination of aqueous extracts of curcuma longa (turmeric) and some calcium channel blockers synergistically improves CCl4-induced hepatotoxicity in albino rats. Pharmacologyonline. 2018; 3:392-401
5. Rahmat AK, Muhammad RK, Sumaira S, Mushtaq A, Naseer AS. Carbon tetrachloride induced lipid peroxidation and hyperglycemia in rat: A novel study. Toxicology and Industrial Health. 2015; 31(6):546-553.
6. Hismiogullari AA, Hismiogullari SE, Karaca O, Sunay FB, Paksoy S, Can M, Seyrek K, Yavvz O. The protective effect of curcumin administration on CCl4- induced nephrotoxicity in rats. Pharmacology Report. 2015; 67:410-416.
7. Uchendu IK, Nnedu EB, Ekeigwe IB, Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats, Journal of Drug Delivery and Therapeutics. 2020; 10(1-s):174-177.
8. Uchendu IK, Ekeigwe IB, Nnedu EB, Antidyslipidaemic and cardioprotective effects of turmeric (Curcuma longa) in rat fed a high cholesterol diet, Journal of Drug Delivery and Therapeutics. 2020; 10(1-s):178-181.
9. Uchendu IK, Agu CE. Anti-nephrotoxic and anti-hyperlipidaemic potentials of aqueous extracts of turmeric (curcuma longa) in hypercholesterolaemic albino rat. Pharmacologyonline. 2018; 3:1-11
10. Uchendu IK, Nnedu EB, Orji OC. Combination of aqueous extracts of Cucuma longa and some calcium channel blockers synergistically improves CCl4-induced hepatotoxicity in albino rats. Pharmacologyonline 2018; 3: 392-401.
11. Kingsley UI. Effects of tomato extract (lycopersicon esculentum) on carbimazole-induced alterations in kidney of albino rats. International Journal of Research and. Review. 2018; 5:72-79.
12. Nnedu EB, Uchendu IK, Orji OC. The role of crude methanol leaf extract of moringa oleifera in protection against hyperlipidaemia and cardiomyopathy in albino rat fed a high cholesterol diet and carbimazole. pharmacologyonline 2018; 3:261-271.
13. Uchendu IK, Agu CE, Orji OC, Nnedu EB, Arinze C, Uchenna AC, Okongwu UC. Effect of tomato (lycopersicon esculentum) extract on acetaminophen - induced acute hepatotoxicity in albino wistar rat. Bioequivalence and Bioavailability International Journal; 2018, 2(1):000119.
14. Orji OC, Uchendu IK, Agu CE, Nnedu EB, Okerreke AN, Orji GC. Combined effects of vitamin c and tomato extract (lycopersicon esculentum) on carbimazole-induced alterations in the testes of male albino rats. Indian Journal of Physiology and Pharmacology. 2018; 62(3):380-384.
15. Ikenna, K.U.; Chidozie, E.A.; Oliver, C.O.; Eluke, B.C.; Ikechukwu, J.C., Nnedu, E.B., Tochi, F.N. and Oluwanifemi, P.A. Effect of Soy (Glycine max) Against Alcohol-Induced Biochemical Alteration in Liver of Male Albino Rat. Der Pharma Chemica; 2017, 9 (16):115-119.
16. Uchendu IK, Orji OC, Agu CE. Attenuation of glycerol-induced acute renal failure in albino rats by soy beans (Glycine max). International Journal of ChemTech Research 2017; 10(12):165-172.
17. Anioke I, Okwuosa C, Uchendu I, Chijioke O, Dozie-Nwakile O, Ikegwuonu I, Kalu P, Okafor M. Investigation into hypoglycemic, antihyperlipidemic, and renoprotective potentials of Dennettia tripetala (Pepper Fruit) Seed in a Rat Model of Diabetes. Hindawi BioMed Research International, 2017; Article ID6923629, 11pages
18. Ikenna, K.U., Okechukwu, K.O., Chidozie, E.A, Oliver, C.O., Blessing, E.C. and Tochi, F.N. Hypolipidaemic and renoprotective effects of glycine max (soy bean) against lipid profile and renal biochemical alterations in hypercholesterolemic rat. International Journal of Biomedical Research. 2016, 7(12):822-828.
19. Kingsley U.I., Steven O.O., Agu C.E.; Orji O.C., Chekwube B.E., Nwosu T. F. Anti-hyperlipidemic effect of crude methanolic extracts of Glycine max (soy bean) on high cholesterol diet-fed albino rats. Journal of Medical and Allied Science., 2017, 7 (1):34-40
20. Babaei HA, Motamedifar M, Khalifat S, Mohammadi A, Khosrow Z, Motamedifar A. In vitro study of antibacterial property and cytotoxic effects of aqueous, ethanolic, methanolic, and hydroalcoholic extracts of fenugreek seed. Pakistan Journal of Medical and Health Sciences. 2018; 12(2): 906-910.
21. Lorke D. A new approach to practical acute toxicity testing. Archive of Toxicology. 1983; 54(4):275-287.
22. Trease G, Evans SM. Pharmacognosy: (15th Edition). English Language Book Society. Bailliere Tindall, London, 2002. pp. 23-67.
23. Reitman S. and Frankel SA. “Colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase”. Am. J. Clin Pathol. 1957; 28:56-58.
24. Kind PR and King EJ. Colorimetric method for determination of serum alkaline phosphatase. J. Clin Pathol. 1954; 7:322.
25. Malloy HT and Evelyn KA. “The determination of bilirubin with the photoelectric colorimetric method”. Journal of Biological Chemistry 1937; 119:481-490.
26. Baker FJ, Silverton RE, Pallister CJ Baker and Silverton’s Introduction to Laboratory Technology. 7th Edition, Butterworth-Heinemann, Wobrun, MA, USA, 1998; page 448.
27. Ding HR, Wang JL, Ren HZ, Shi XL. Lipometabolism and glycometabolism in liver diseases. BioMedical Research International. 2018; 2018: ID 1287127, 7 pages.
28. Champe PC, Harvey RA, Ferrier DR. Lippincotts Illustrated Reviews: Biochemistry, 4th Edition. Wolters Kluwer Health, Lippincott Williams and Wilkins, New York. 2008; pp251-284.
29. Cox MM, Nelson DL. Lehninger Principle of Biochemistry 4th Edition. W. H. Freeman and Company, New York. 2005; pp238-276.
30. Allen JH. The wicked problem of chemicals policy: opportunities for innovation. Journal of Eenvironmental Science 2013; 3:101-8
31. Schyman P, Printz RL, Estes SK, , Boyd KL, Shiota M, Wallqvist A. Identification of the toxicity pathways associated with thioacetamide-induced injuries in rat liver and kidney. Frontiers of Pharmacology. 2018; 9:1272.
32. Ajibesin KK. Dacryodes edulis (G. Don) HJ. Lam: A review on its medicinal, phytochemical and economic properties. Research Journal of Medicinal Plant 2011; 5(1):32-41.
33. Ekeigwe IB, Ikegwuonu IC, Uchendu IK, Uchenna CA , Okongwu UC. Curcuma longa aqueous extract prevents myocardial injury in hypercholesterolaemic albino rat. Ukrainian Biochemical Journal. 2019; 91(4):50-57.
34. Bourgou S, Pichette A, Marzouk B, Legault J. Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany. 2010; 76:210-216.
35. Pieta PG. Flavonoids as antioxidants. Journal of Natural Products. 2000; 63(7):1035-1042.
36. Essawy AE, Abdel-Moneim AM, Khayyat L, Elzergy AA. Nigella sativa seeds protect against hepatotoxicity and dyslipidaemia induced by carbon tetrachloride in mice. Journal of Applied Pharmaceutical Science. 2012; 2 (10):21-25.
37. Al-Razzuqi R, Hussaini J, Al-Jeboori A. A protective effect of nigella sativa against carbon tetrachloride-induced liver injury in experimental rabbit models. International Journal of Green Pharmacy 2011; 5(3):198-200.
38. Ismail N, Ismail M, Azmi HN, Bakar AFM, Basri H, Abdullah AM. Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by thymoquinone-rich fraction and thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes. Oxidative Medicine and Cellular Longevity. 2016; 2016:2528935.
39. Paarakh MP. Nigella sativa Linn – A comprehensive review. Indian Journal of Natural Products and Resources. 2010; 1(4):409-429.
40. Khan AM, Afzal M. Chemical composition of Nigella sativa Linn: part 2 recent advances. Inflammopharmacology. 2016; 24:67-79.
41. Saleh FA, El-Darra N, Raafat K, Ghazzawi IE. . Phytochemical analysis of Nigella sativa L. Utilizing GC-MS exploring its antimicrobial effects against multidrug-resistant bacteria. Pharmacogsy Journal. 2018; 10(1):99-105.
42. Ahmadi M, Scurtu M, Tulcan C, Boldura O, Milovanov C, Hutu I, Mircu C, Radulov I, Dronca D. Nigella sativa – a plant with personality in biochemistry and experimental medicine researches. Bulletin UASVM veterinary medicine. 2016; 73(2):203-209.
43. Darakhshan S, Pour AB, Colagar AH, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacological. Research. 2015; 95-96:138-158.
44. Cherrak SA, Mokhtari-Soulimane N, Berroukeche F, Bensenane B, Cherbonnel A, Merzouk H, Elhabiri M. In vitro antioxidant versus metal ion chelating properties of flavonoids: A structure-activity investigation. Plos One. 2016; 11(10):e0165575.
45. Mahmoud AM, Bautista RJH, Sandhu MA, Hussein OE. Beneficial effects of citrus flavonoids on cardiovascular and metabolic health. Oxidative Medicine and Cellular Longevity. 2019; 2019:19.
46. Kooti W, Hasanzadeh-Nooti Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, Pharmacology and therapeutic uses of black seed (Nigella Savita). Chin J Nat Medicines. 2016; 14(10):732-745.
47. Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. Journalof Nutritional Science, 2016; 5:1-15.
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Uchendu IK. Ameliorative effect of hydroalcoholic extracts of Nigella sativa seed against CCl4-induced acute liver injury in rats. JDDT [Internet]. 15May2020 [cited 1Jun.2020];10(3):164-9. Available from: