A Highly Specific Colorimetric Method for On-Spot Determination of Lidocaine Using Color Kit and Application of Uncertainty Principles

  • Margi Gandhi Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA
  • Rajashree Mashru Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA

Abstract

A simple, accurate, precise, selective and detectable Colorimetric method was developed for estimation of Lidocaine in five different Pharmaceutical Formulations. The method is an azo C-coupling reaction where Lidocaine undergoes series of reaction and finally couples with resorcinol to form yellow color azo compound. The colored complex was measured at 430 nm. Beers law was obeyed in concentration range of 0.05-0.8 ug/ml. The method was validated and was found to be accurate, precise and robust with limit of detection and quantification to be 0.014 and 0.045 ug/ml. The Color kit was also developed for On-Spot detection of Lidocaine. Measurement uncertainty principles were also adopted to obtain reliable results where the process of uncertainty started from specifying measurand, then identifying uncertainty sources by cause-effect diagram, quantification of these sources of uncertainty and then finally calculating combined standard uncertainty and expanded uncertainty. In the present experiment, Concentration of sample and mass of sample was the major contributor towards uncertainty for all five Formulations. From the five formulations combined standard uncertainty of Transdermal patch was high, followed by Aerosol, Ointment, Gel and Injection.


Keywords: Lidocaine, Uncertainty, Transdermal patch, Aerosol, Injection, Ointment, Gel

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Author Biographies

Margi Gandhi, Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA

Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA

Rajashree Mashru, Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA

Faculty of Pharmacy, G.H. Patel Building, Donors Plaza, The M.S. University Of Baroda, Vadodara, 390002, INDIA

References

1) Lazeeza Sattar Omer, Rasul Jameel Ali, Extraction-Spectrophotometric Determination of Lidocaine Hydrochloride in Pharmaceuticals, International Journal of Chemistry; 2017; 9(4).
2) Edhorn, G. A; Pharx, M. Determination of lidocaine in whole blood by gas chromatography. Canad. Anaesth. Sot.. J. 1971; 18(2).
3) Sporkert , F; Pragst , F. Determination of Lidocaine in Hair of Drug Fatalities by Headspace Solid-Phase Microextraction. Journal of Analytical Toxicology. 2000; Vol. 24, July/August.
4) Janguo, L; Huangxia, J. Simultaneous determination of ethamsylate, tramadol and lidocaine in human urine by capillary electrophoresis with electrochemiluminescence detection. Electrophoresis. 2006; 27:3467–3474.
5) Zonghui, Q; Guangming, S; Rong, T; Lijun, P. Determination of the Content of Lidocaine Hydrochloride in Different Medium by Spectrophotometric Method. China Pharmacy. 2007; 34.
6) Sasa, I ; Dada, Y; Lnln, G. Spectrophotometric determination of lidocaine in some pharmaceutical preparations using bromocresol green. Pharmazie. 1975; 408. 30, 6, PMID 1161799.
7) Putta, M; Gurupadayya, B. M; Disha, N. S; Sama, S. Spectrophotometric determination of lidocaine in bulk and pharmaceutical formulations. Pharm Analysis & Quality Assurance. 2013; 02:573.
8) Qin, Z; Pu, L; Tan, R. Spectrophotometric Method for Determining Lidocaine Hydrochloride with Eosin Methylene Blue. Journal of Jiangxi Normal University. 2007; 03
9) Dr. A.J.Pratapareddy ,New Spectrophotometric Determination Ofmetoclopramide In Bulk And Pharmaceutical Dosage Form, Journal of Drug Discovery and Therapeutics, 2015; 3(35):01-04
10) ICH Q2 (R1): Validation of analytical procedure, Text and methodology, Geneva, International conference on Harmonization, 2005.
11) Sevtap Karakurt, Serdar Karakurt , Measurement Uncertainty Of Spectrophotometric Determination Of Hexavalent Chromium In Water , U.P.B. Sci. Bull., Series B, 2017; 79(4).
12) Karan Mittal, Riddhish Patadia, Chintan Vora, Rajashree C Mashru , A newfangled study using risk silhouette and uncertainty approximation for quantification of acyclovir in diverse formulation , Journal of Pharmaceutical Analysis, June 2014.
13) Krishna Jadav, Rajashree C. Mashru, Risk assessment and uncertainty appraisal for quantification of Aripiprazole and lamotrigine in combination: A neoteric validation approach, The Pharma Innovation Journal 2019; 8(4):538-548.
14) Eurachem/Citac, CG 4 Quantifying Uncertainty in Analytical Measurement Third Edition, 2012.
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Gandhi M, Mashru R. A Highly Specific Colorimetric Method for On-Spot Determination of Lidocaine Using Color Kit and Application of Uncertainty Principles. JDDT [Internet]. 15Mar.2020 [cited 8Jul.2020];10(2):86-. Available from: http://jddtonline.info/index.php/jddt/article/view/3970