Preparation and Characterization of Celecoxib Entrapped Guar Gum Nanoparticles Targeted for Oral Drug Delivery against Colon Cancer: An In-Vitro Study

  • Deepika Chandel Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India
  • Shivani Uppal Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India
  • Surinder Kumar Mehta Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India
  • Geeta Shukla Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Abstract

The present study is an attempt to synthesize nanosized guar gum carriers encapsulating celecoxib as the chemopreventive agent for experimental colorectal cancer (CRC).  Guar gum nanoparticles without celecoxib (eGGNPs) and celecoxib loaded guar gum nanoparticles (cGGNPs) were prepared by oil-in-water emulsification and in situ polymer crosslinking method. Electron microscopy, zeta potential and fourier transform infrared spectra analysis was used to affirm the size, stability and morphology of the nanoparticles. In-vitro drug release was investigated using dialysis method. Further, the effect of nanoparticles (eGGNPs & cGGNPs) was evaluated on Caco 2 colon cancer cell lines. Spherical guar gum nanoparticles were obtained in the size range of 200±6nm with zeta potential of -32.1mV indicating good stability of the GGNPs with drug loading of 30±3.2%, and drug release following zero order kinetics. The eGGNPs had no effect on Caco2 cell viability whereas the cGGNPs showed time and concentration dependent growth inhibition of Caco 2 cells. These findings suggest the successful preparation of chemopreventive nanoparticles that can be targeted as the prophylactic agent for experimental colorectal cancer.


Keywords: nanoparticles, guar gum, celecoxib, colorectal cancer, release kinetics, cytotoxicity

Keywords: nanoparticles, guar gum, celecoxib, colorectal cancer, release kinetics, cytotoxicity

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Author Biographies

Deepika Chandel, Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Shivani Uppal, Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Surinder Kumar Mehta, Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Geeta Shukla, Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

Department of Microbiology, Basic Medical Sciences Block I, South Campus, Sector 25, Panjab University, Chandigarh 160014, India

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Chandel D, Uppal S, Mehta SK, Shukla G. Preparation and Characterization of Celecoxib Entrapped Guar Gum Nanoparticles Targeted for Oral Drug Delivery against Colon Cancer: An In-Vitro Study. JDDT [Internet]. 15Apr.2020 [cited 16Aug.2022];10(2-s):14-1. Available from: http://jddtonline.info/index.php/jddt/article/view/3951