Allelopathic effect of leaf extracts of Punica granatum and Spiraea prunifolia against post-harvest rot of tomato and brinjal

  • Jahangir Abdullah Koka Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India
  • Mohd Yaqub Bhat Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India
  • Abdul Hamid Wani Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India

Abstract

Antimycotic activities of different solvent extracts of Punica granatum L. and Spiraea prunifolia Siebold and Zucc. were carried out through agar well diffusion assay at three concentrations (25µl, 50µl and 75µl) against seven fungi causing rot diseases of tomato and brinjal. All the concentration of plant extracts showed antifungal activity against tested pathogenic fungi. Antimycotic activity increased with the increased concentrations of plant extracts. However, higher concentrations proved more effective than lower concentrations. It was revealed from the present study that the ethanolic extract of Punica granatum L. showed maximum antifungal activity against Rhizoctonia solani and Penicillium expansum and least inhibitory activity against Aspergillus niger. However, the aqueous extract of Punica granatum L. showed maximum antimycotic activity against Rhizoctonia solani and Alternaria alternata and least inhibitory effect against Penicillium expansum. It was further observed from the present study that the ethanolic extract of Spiraea prunifolia Siebold and Zucc. showed maximum antimycotic activity against Rhizoctonia solani and least inhibitory effect against Alternaria alternata. Whereas the aqueous extract of Spiraea prunifolia Siebold and Zucc showed maximum antifungal activity against Aspergillus niger and least inhibitory activity against Alternaria alternata.


Keywords: Plant extracts, Concentration, Antimycotic effectiveness, tomato and brinjal, fungal rot pathogens, Inhibition zone.

Keywords: Plant extracts, Concentration, Antimycotic effectiveness, tomato and brinjal, fungal rot pathogens, Inhibition zone

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Author Biographies

Jahangir Abdullah Koka, Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India

Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir,   Hazratbal, Srinagar, India

Mohd Yaqub Bhat, Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India

Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir,   Hazratbal, Srinagar, India

Abdul Hamid Wani, Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir, Hazratbal, Srinagar, India

Section of Plant Pathology and Mycology, Department of Botany, University of Kashmir,   Hazratbal, Srinagar, India

References

1. Snowdon AL. Postharvest diseases and disorders of fruits and vegetables. Vol.1. General Introduction and fruits. Boca Raton, F.L. CRC Press.1992. pp.302.
2. Naqvi SAMH. Diseases of fruits and vegetables, Kluwer Academic Publishers, Netherland, 2004. 2: 511-535.
3. El-Ghaouth A. Wilson CL. Wisniewski ME. Biologically based alternatives to synthetic fungicides for the postharvest diseases of fruit and vegetables. In: Naqvi, S. A. M. H. (Ed.), Diseases of Fruit and Vegetables, Kluwer Academic Publishers, The Netherlands, 2004. 2: 511–535.
4. Droby S. Improving quality and safety of fresh fruit and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Horticulturae, 2006. 709: 45–51.
5. Singh D. Sharma RR. Postharvest diseases of fruit and vegetables and their management. In: Prasad, D. (Ed.), Sustainable Pest Management. Daya Publishing House, New Delhi, India.2007.
6. Bhowmik D. Gopinath H. Pragati Kumar B. Duraivel S. Aravind G. Sampath Kumar KP. Medicinal uses of Punica granatum and its health benefits. Journal of Pharmacognomy and Phytochemistry, 2013. 1(5):28-35.
7. Facciola S. Cornucopia: a Source Book of Edible Plants. Kampong PublicatioCalifornia. (1990) 166
8. Elgorashi EE. Van Staden J. Pharmacological screening of six Amaryllidaceae species. Journal of Ethnopharmacology, 2004. 90:27-32.
9. Broekaert WF. Terras FRG. Cammue BPA. Vanderleyden J. An automated quantative assay for fungal growth inhibition. FEMS Microbiology Letters, 1990. 69:55-60.
10. Abril M. Curry KJ. Smith BJ. Wedge DE. Improved microassays used to test natural product based and conventional fungicides on plant pathogenic fungi. Plant dis. 2008. 92: 106-112.
11. Perez C. Pauli M.Bazerque P. An antibiotic assay by the well agar method. Acta Biolog, Med. Experiment.1990; 15:113-115.
12. Alzoreky NS. Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International Journal of Food Microbiology 2003.80:223-230.
13. Ahmad N. Amir MK. Ayaz S. Ahmad JA. Antimicrobial profile of the selected medicinal plants. Int J Chem Lif. Sci. 2012. 01:1039-1041.
14. Norrel SA. Messley KE. Microbilogy laboratory Manual Principlesw and Applications,Prentice Hall, Upper Saddle River. New Jersey. 1997.85-90.
15. Ganie SA. Ghani MY. Qazi N. Shabir-u-Rehman. Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. African Journal of Microbiology Research, 2013. 7(34): 4397-4402.
16. Mehmood T. Shafique S. Shafique S. Zaheer Z. Fungitoxic potential of Tagetus erectus for the management of Alternaria alternata strains of tomato. Pak. J. Bot. 2014. 46(3): 1047-1054.
17. Akpa AD. Musa B. Paswall AT. Effect of Neem extracts on mycelial growth of the sorghum anthrachnose pathogen, Collectotrichum graminicola Proc. 21st Annual Conference of Nigerian Society for Plant Protection, 1991. 10-13 March, pp 47.
18. Tijjani A. Gurama AU. Aliyu M. In vitro and In vivo evaluation of some plant extracts for the control of wet rot disease of potato caused by Rhizopus stolonifer. Journal of league of Researchers of Nigeria, 2010. 11(2):45-49.
19. Kareem SO. Akpan I. Ojo OP. Antimicrobial Activities of Calotropis procera on selected pathogenic microorganisms. African J. Biomedical Res. 2008. 11: 105– 110.
20. Rizk MA. Phytotoxic effect of Calotropis procera extract on seedling development and rhizosphere microflora of tomato plants in soil infested with Fusarium oxysporum f. sp. lycopersici. World Appl. Sci. J. 2008. 3(3):391-397.
21. Venkatesan S. Subramanian SP. Evaluation of antifungal activity of Calotropis gigantea latex extract: An in vitro study. Internat. J. Pharmaceut. Sci. and Res. 2010. 1 (9):88-96.
22. Moubasher AH. Elnaghy MA. Megala SE. Fungi isolated from sclerotia of Sclerotium cepivorum and from soil and their effects upon the pathogen. Plant and Soil, 1970.33: 305-312.
23. Singh UP. Pandey VN. Wagner KG. Singh KP. Antifungal activity of ajoene, a constituent of garlic (Allium sativum).Can. J. Bot.1990. 68: 1354-1356.
24. Hasan MM. Chowdhury SP. Alam S. Hossain B. Alam MS. Antifungal effects of plant extracts on seed-borne fungi of wheat seed regarding seed germination, Seedling health and vigour index. Pak. J. Biol. Sci. 2005. 8: 1284-1289.
25. Tagoe DNA. Nayar HD. Akpaka RA comparison of antifungal properties of onion (Allium cepa), Ginger (Zingiber officinale) and Garlic (Allium sativum) against Aspergillus flavus, Aspergillus niger and Cladosporium herbarum. Res. J. Med. Plant, 2011. 5: 281-287.
26. Daya R. Ram D. Fungitoxic properties of some plant extracts against Alternaria brassicae. Ann. Agri. Biol. Res. 1997. 2:25-26.
27. Mondall NK. Mojumdar A. Chatterje SK. Banerjee A. Datta JK. Gupta S. Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal of Applied Sciences and Environmental Management, 2009. 13(1): 49-53.
28. Saheb SL. More SM. Junne SB. Wadje SS. The antifungal activity of five Terminalia species checked by paper disc method. International Journal of Pharma Research and Development. 2011.3(2):36-40.
29. Senguttuvan J. Paulsamy S. Krishnamoorthy K. In vitro antifungal activity of leaf and root extracts of the medicinal plant, Hypochaeris radicata L. International Journal of Pharmacy and Pharmaceutical Sciences, 2013. 5(3): 758-761.
30. Mangang HC. Chhetry GKN. Antifungal properties of certain plant extracts against Rhizoctonia solani causing root rot of French bean in organic soil of Manipur. International Journal of Scientific and Research Publications, 2012. 2 (5): 1-4.
31. Tijjani A. Adebitan SA. Gurama AU. Aliyu M. Haruna SG. Mohammad GU. Musab I. In vitro and in vivo efficacy of some plant extracts for the control of tomato fruit rot caused by Aspergillus flavus. International Journal of Scientific and Research Publications, 2014. 4(4):1-5.
32. Parveen S. Wani AH. Ganie AA. Pala SA. Mir RA. Antifungal activity of some plant extracts on some pathogenic fungi. Archives of Phytopathology and Plant Protection, 2014. 47:279–284, Doi: 10.1080/03235408.2013.808857.
33. Koka JA. Wani AH. Bhat MY. Parveen S. Antifungal Activity of Ethanolic and Aqueous Leaf Extracts of Taraxicum officinale and Mentha arvensis on the growth of some selected fungal species under in vitro Conditions, Int. J. Pure App. Biosci., 2017. 5 (5):1170-1176.
34. Koka JA. Wani AH. Bhat MY. Wani TA. Parveen S. Antimycotic activity of ethanolic and aqueous leaf extracts of Ajuga bracteosa Wall. Ex Benth. (Lamiale: Lamiaceae) and Iris kashmiriana Baker (Asparagales: Iridaceae) against some vegetable rot fungi. Brazilian Journal of Biological Sciences, 2018. 5 (9):75-84
35. Satpute SB. Vanmare DJ. In vitro antifungal activity of Tamarindus indica L. extract against pathogenic fungi. International Journal of Botany Studies, 2017.2(3): 25-28.
36. Jantasorn A. Moungsrimuangdee B. Dethoup T. In vitro antifungal activity evaluation of five plant extracts against five plant pathogenic fungi causing rice and economic crop diseases. Journal of Biopesticides, 2016. 9(1): 01-07.
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1.
Koka JA, Bhat MY, Wani AH. Allelopathic effect of leaf extracts of Punica granatum and Spiraea prunifolia against post-harvest rot of tomato and brinjal. JDDT [Internet]. 15Apr.2020 [cited 16Aug.2022];10(2-s):1-. Available from: http://jddtonline.info/index.php/jddt/article/view/3948