The Phytochemical and Comparative Anticancer Study of Methanolic and Chloroform Extracts of Psidium guajava L. Leaves of Pakistani Origin

  • Muhammad Abbas Assistant Professor, Islam College of Pharmacy, Sialkot, Pakistan
  • Muhammad Tayyab Ansari Professor, Department of Pharmaceutical Chemistry, Bahauddin Zakariya University, Multan, Pakistan
  • Saeed ul Hassan Faculty of Pharmacy, University of Lahore, Lahore-51550, Pakistan
  • Muhammad Nadeem Alvi Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan
  • Musharraf Abbas Lahore Pharmacy College, Lahore, Pakistan

Abstract

The chief focus of our study is to evaluate the phytochemical and anti-cancer activity of methanol (PGM) and chloroform extracts (PGC) of the leaves of Psidium guajava (guava) collected from local area of district Sialkot, Pakistan. Shade dried milled leaves was subjected to extraction (maceration) with methanol and chloroform. Quantitative and qualitative screenings by GC-MS and phytochemical techniques were performed. Then different secondary metabolites and phytochemical compounds were identified which are typically associated with the existence of therapeutic characteristics. Psidium guajava has been extensively used as herbal remedies like, anti-diarrheal, antihypertensive, antibacterial, antifungal as well as to control obesity, ulcer, diabetes. In this study, both extracts of P. guajava were evaluated for their anticancer activities against HeLa cell-lines (cancerous cells). The healthiest anticancer response in the form of cell-line suppression was perceived with 200µg/mL of both extracts, PGM showed 81% and PGC exhibited 91% while the standard drug doxorubicin presented around 76% inhibition. The comparative better result was seen with chloroform extract than methanolic abstract. In conclusion, the chloroform and methanol extracts of our nominated plant from Pakistan origin has a good source of phytochemicals that revealed an outstanding anti-cancer potential.


Keywords: Psidium guajava, anticancer, phytochemicals, methanol extracts, secondary metabolites.

Keywords: Psidium guajava, anticancer, phytochemicals, methanol extracts, secondary metabolites

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Author Biographies

Muhammad Abbas, Assistant Professor, Islam College of Pharmacy, Sialkot, Pakistan

Assistant Professor, Islam College of Pharmacy, Sialkot, Pakistan

Muhammad Tayyab Ansari, Professor, Department of Pharmaceutical Chemistry, Bahauddin Zakariya University, Multan, Pakistan

Professor, Department of Pharmaceutical Chemistry, Bahauddin Zakariya University, Multan, Pakistan

Saeed ul Hassan, Faculty of Pharmacy, University of Lahore, Lahore-51550, Pakistan

Professor, Faculty of Pharmacy, University of Lahore, Lahore, Pakistan

Muhammad Nadeem Alvi, Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan

Assistant Professor, Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan

 

Musharraf Abbas, Lahore Pharmacy College, Lahore, Pakistan

Lecturer, Lahore Pharmacy College, Lahore, Pakistan

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Abbas M, Ansari M, Hassan S, Alvi M, Abbas M. The Phytochemical and Comparative Anticancer Study of Methanolic and Chloroform Extracts of Psidium guajava L. Leaves of Pakistani Origin. JDDT [Internet]. 15Feb.2020 [cited 31May2020];10(1-s):149-53. Available from: http://jddtonline.info/index.php/jddt/article/view/3922