Acute and Sub-acute Toxic Effects of Algerian Peganum harmala L. Crud Extract

  • Abir Rezzagui Laboratory of Applied Biochemistry : Oxidative stress and Inflammation. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria
  • Mounira Merghem Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria
  • Ismahane Derafa Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria
  • Saliha Dahamna Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Abstract

The present study was carried out to evaluate the acute and sub-acute toxicity of Algerian Peganum harmala L. seeds (Zygophyllaceae). For the acute toxicity assessment, oral administration of P. harmala crud extract to mice in single doses of 0-12 g/kg caused dose-dependent general behavior adverse effects and mortality with LD50 of 2,86 g/kg. In the sub-acute study, daily oral administration of the crude aqueous extract for 28 consecutive days did not result no mortality. However, our results showed a significant difference in organs weights between control and treated animals. In the biochemical analysis, significant changes occurred in Bilirubin, uric acid (UA) and alkaline phosphatase (ALP) in the treated animals when compared to the control ones. Hematological analysis showed differences in WBC count and Hemoglobin estimation. However, urinalysis was negative for all parameters except Glucose in the treated group. Pathologically, a few gross abnormalities and histological changes were observed in hepatic and nephritic tissues.


Keywords: Peganum harmala L., Acute and Sub-Acute Toxicity, Hematological parameters, Biochemical parameters

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Author Biographies

Abir Rezzagui, Laboratory of Applied Biochemistry : Oxidative stress and Inflammation. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Laboratory of Applied Biochemistry : Oxidative stress and Inflammation. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Mounira Merghem, Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Ismahane Derafa, Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Saliha Dahamna, Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases. Department of Animal Biology and Physiology. Faculty of Natural and Life Sciences. University Ferhat Abbas. Sétif - 19000 Algeria

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Rezzagui A, Merghem M, Derafa I, Dahamna S. Acute and Sub-acute Toxic Effects of Algerian Peganum harmala L. Crud Extract. JDDT [Internet]. 15Mar.2020 [cited 9Apr.2020];10(2):115-21. Available from: http://jddtonline.info/index.php/jddt/article/view/3920