Chemical composition and antibacterial activity of Algerian propolis against fish pathogenic bacteria

  • El-Khamsa Soltani Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria
  • Kamel Mokhnache Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria
  • Samia Mezaache-Aichour Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria
  • Noureddine Charef Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria
  • Juan Pedro De Haro Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain
  • Mohamed Mihoub Zerroug Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria
  • Maria Angeles Esteban Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain

Abstract

Five different varieties of propolis from four sites from Sétif region (East of Algeria) (Babor, Setif; Ain-Abbassa and El-Hamma), and one site from the center of Algeria (Tizi-Ouzou) were chemically analysed by gas chromatography-mass spectrometry. One hundred and two compounds were identified including aromatic acids, linear hydrocarbons and their acids, terpenes and alcaloïdes. Furthermore, the in vitro bacteriostatic and bactericidal activities of the aqueous extracts were evaluated against one Gram positive (Bacillus subtilis, used as probiotics in aquaculture) and two Gram negative (Vibrio anguillarum and Vibrio harveyi, pathogenic for fish) bacteria. The obtained results showed that all aqueous extracts of propolis inhibit the growth of B. Subtilis while the growth inhibition of fish pathogens was achieved when using higher propolis concentrations. These antibacterial properties would warrant further studies on the clinical applications of propolis in aquaculture field.


Keywords: Bactericidal activity; Chemical characterization; Propolis; Vibrio.

Downloads

Download data is not yet available.

Author Biographies

El-Khamsa Soltani, Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Kamel Mokhnache, Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Samia Mezaache-Aichour, Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Noureddine Charef, Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Laboratory of Applied Biochemistry, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Juan Pedro De Haro, Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain

Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain

Mohamed Mihoub Zerroug, Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Laboratory of Applied Microbiology, faculty of nature and Life sciences, University Ferhat Abbas Setif 1, 19000, Setif, Algeria

Maria Angeles Esteban, Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain

Fish innate immune system group. Department of Cell Biology & Histology, Faculty of Biology. Regional Campus of International Excellence "Campus Mare Nostrum". University of Murcia, 30100 Murcia, Spain

References

1. Ghisalberti, E.L., Bee World 1979; 60:59(https://doi.org/10.1080/0005772X.1979.11097738)
2. Burdock, G.A., Food Chem. Toxicol. 1998; 36:347 (http://dx.doi.org/10.1016/S0278-6915(97)00145-2)
3. Bankova, V.S., De Castro, S.L., Marcucci, M.C., Apidologie, 2000 ; 31 :3(https://doi.org/10.1051/apido:2000102)
4. Ivanovska, N.D., Dimov, V.B., Bankova, V.S., Popov, S.S., J Ethnopharmacol. 1995 ; 47 :145 (https://doi.org/10.1016/0378-8741(95)01272-F)
5. Park, Y.K., Koo, M.H., Abreu, J.A.S., Ikegaki, M., Cury, J.A., Rosalen, P.L., Curr. Microbiol. 1998; 36:24 (https://doi.org/10.1007/s002849900274)
6. Trusheva, B., Trunkova, D., Bankova, V., Chem. Cent. J. 2007; 1:13 (https://doi.org/10.1186/1752-153X-1-13)
7. Mello, B.C.B.S., Petrus, J.C.C., Hubinger, M.D., J. Food Eng. 2010; 96:533 (https://doi.org/10.1016/j.jfoodeng.2009.08.040)
8. Nadia, B.H., Wided, K., Kheira, B., Hassiba, R., Lamia, B., Rhouati, S., Alyane, M., Zellagui, A., Lahouel, M., Acta Biol Hung. 2009; 60(4):385 (https://doi.org/10.1556/ABiol.60.2009.4.5)
9. Piccinelli, A.L. Mencherini, T., Celano, R., Mouhoubi, Z., Tamendjari, A., Aquino, R.P.,Rastrelli, L., J Agric Food Chem. 2013 ; 61(21):5080 (https://doi.org/10.1021/jf400779w)
10. Gülçin, I., Bursal, E., Sehitoglu, M. H., Bilsel, M., Gören, A. C., Food ChemToxicol. 2010; 48:2227(https://doi.org/10.1016/j.fct.2010.05.053)
11. Popova, M., Silic, S. i, Kaftanoglu, O., Bankova, V.S., Phytomedicine 2005; 12:221
(http://dx.doi.org/10.1016/j.phymed.2003.09.007 )
12. Rico, R.M., Tapia-Paniagua, S., Martínez-Manzanares, E., Balebona, M.C. Moriñigo, M.A., J ApplMicrobiol. 2008; 105(3):752 (https://doi.org/10.1111/j.1365-2672.2008.03786.x)
13. Andrews, J.M., J AntimicrobChemother. 48 (1) (2001) 5 (https://doi.org/10.1093/jac/dkf083)
14. Salomao, K., Dantas, A.P., Borba, C.M., Campos, L.C., Machado, D.G., Aquino Neto, F.R., de Castro, S.L., Lett. Appl. Microbiol. 2004; 38:87 (https://doi.org/10.1111/j.1472-765X.2003.01458.x)
15. Pellati, F., Prencipe, F.P., Benvenuti, S., J Pharmaceut Biomed. 2013; 84:103 (https://doi.org/10.1016/j.jpba.2013.05.045)
16. Mohammadzadeh, S., Shariatpanahi, M., Hamedi, M., Ahmadkhaniha, R., Samadi, N., Ostad, S.N., Food Chem. 2007; 103:1097 (https://doi.org/10.1016/j.foodchem.2006.10.006)
17. Isidorov, V.A., Szczepaniak, L., Bakier, S. Food Chem. 2014; 142:101 (https://doi.org/10.1016/j.foodchem.2013.07.032)
18. Abd-El-Rhman, AM., Fish Shellfish Immunol. 2009; 27(3):454 (https://doi.org/10.1016/j.fsi.2009.06.015)
19. Tukmechi1, A., Ownagh, A., Mohebbat, A., Braz J Microbiol. 2010; 41:1086 (https://doi.org/10.1590/S1517-83822010000400030)
20. Sulaiman, G.M., Al Sammarrae, K.W., Ad’hiah, A.H., Zucchetti, M., Frapolli, R., Bello, E., Erba, E., D’Incalci, M., Renzo, B., Food ChemToxicol. 2011; 49:2415 (https://doi.org/10.1016/j.fct.2011.06.060)
21. Cuesta, A., Rodríguez, A., Esteban M.A., Meseguer, J., Fish Shellfish Immunol. 2005; 18(1):71(https://doi.org/10.1016/j.fsi.2004.06.002)
22. EnisYonar, M., MişeYonar, S., Silici, S., Fish Shellfish Immunol. 2011 ; 31(2)318 (https://doi.org/10.1016/j.fsi.2011.05.019)
23. Cañada-Cañada, F., Muñoz de la Peña, A., Espinosa-Mansilla, A., Anal Bioanal Chem. 2009; 395(4):987 (https://doi.org/10.1007/s00216-009-2872-z)
24. Banskota, A.H., Tezuka, Y., Kadota, S., Phytother. Res. 2001 ; 15 :561 (https://doi.org/10.1002/ptr.1029)
25. Aly, S.M., Abdel-Galil Ahmed, Y., Abdel-Aziz Ghareeb, A., Mohamed, M.F.,Fish Shellfish Immunol. 2008; 25(1-2):128 (https://doi.org/10.1016/j.fsi.2008.03.013)
26. Salinas, I., Cuesta, A., Esteban M.A., Meseguer. J., Fish Shellfish Immunol. 2005; 19(1):67 (https://doi.org/10.1016/j.fsi.2004.11.007)
27. Salinas, I., Abelli, L., Bertoni, F., Picchietti, S., Roque, A., Furones, D., Cuesta, A., Meseguer, J., Esteban, M.A., Fish Shellfish Immunol. 2008; 25(1-2):114 (https://doi.org/10.1016/j.fsi.2008.03.011)
28. Cerezuela, R., Fumanal, M., Tapia-Paniagua, S.T., Meseguer, J., Moriñigo, M.A., Esteban, M.A., Cell Tissue Res. 2012; 350(3):477 (https://doi.org/10.1007/s00441-012-1495-4)
29. Cerezuela, R., Fumanal, M., Tapia-Paniagua, S.T., Meseguer, J., Moriñigo, M.Á., Esteban,M.Á., Fish Shellfish Immunol. 2013; 34(5):1063 (https://doi.org/10.1016/j.fsi.2013.01.015)
30. Petersen, A., Andersen, J.S., Kaewmak, T., Somsiri, T., Dalsgaard, A., Appl. Environ. Microbiol. 2002 ; 68(12) :6036 (https://doi.org/10.1128/aem.68.12.6036-6042.2002)
31. Marcucci, M.C., Ferreres, F., Garc a-Viguera, C., Bankova, V.S., De Castro, S.L., Dantas, A.P., alente, P.H.M., Paulino, N., J Ethnopharmacol. 2001; 74:105 (https://doi.org/10.1016/S0378-8741(00)00326-3)
32. Takaisi, N.B., Scjoncjer, H., Planta Med. 1994; 60:222 (http://dx.doi.org/10.1055/s-2006-959463)
33. Cushnie, T.P.T., Lamb, A.J., J Ethnopharmacol. 2005; 101:243 (https://doi.org/10.1016/j.jep.2005.04.014)
Statistics
26 Views | 40 Downloads
How to Cite
1.
Soltani E-K, Mokhnache K, Mezaache-Aichour S, Charef N, Haro JPD, Zerroug MM, Esteban MA. Chemical composition and antibacterial activity of Algerian propolis against fish pathogenic bacteria. JDDT [Internet]. 15Mar.2020 [cited 8Jul.2020];10(2):12-9. Available from: http://jddtonline.info/index.php/jddt/article/view/3904