A Comprehensive Review on Plant derived Natural products for Diabetes and its complication as nephropathyExtraction and Quantitative Estimation of Chrysophanol From Cassia tora Meal Powder by High-Performance Liquid Chromatography
An isocratic reverse phase HPLC method were described to determined chrysophanol contain in cassia tora meal powder obtained during the split manufacturing process of cassia tora seed husk and germs which are used as a valuable cattle feed and poultry feed as they are quite rich in protein. The method involves methanol: water (90:10) with 0.05% Ortho-Phosphoric acid as solvent system and chromatogram detection was done on 254 nm using photodiode array detector. Chrysophanol were extracted using methanol, 70% methanol, acid hydrolysis and Oxidative hydrolysis by different concentration of the Hydrochloric acid and ferric chloride respectively. An average chrysophanol content was observed in cassia tora meal powder was 0.066 percent. In an efforts to develop an improved method for the determination of content of chrysophanol which is important marker compound in cassia tora meal and also improve method of an extraction methods. The solvents for extraction of chrysophanol from cassia tora were examined in order to increase the chrysophanol contents in cassia tora meal powder. It was found that 70% methanol, 20% HCL (v/v) and 3% FeCl3 (v/v) was capable of increasing the chrysophanol contain up to 0.099 percent.
Keywords: HPLC, Chrysophanol, Extraction, Cassia tora meal powder
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