Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats

  • Ikenna Kingsley Uchendu Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria
  • Ebuka Bitrus Nnedu Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria
  • Ifeoma Blessing Ekeigwe Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Abstract

Objective:  The aim of this study is to investigate the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cardiotoxicity of cadmium in albino rats.


 Methods: Twenty five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Cardiac injury was assessed by measuring serum levels of Aspartate aminotransferase (AST), Lactate dehydrogenase (LDH) and Creatine kinase (CK-MB) using standard methods. The heart was harvested for histopathological examination.


Results: CdCl2 induced significant cardiotoxicity with marked elevation in the levels of biochemical markers of cardiac functions (p<0.05 or p<0.01); these were however attenuated by MENS. Histopathological examination of the heart sections supported the biochemical findings.


Conclusion: Nigella sativa seed extract is potentially cardioprotective against harmful chemical toxins such as cadmium.


Keywords: Cadmium, CdCl2, Nigella sativa, Medicinal food, cardiotoxicity

Keywords: Cadmium, CdCl2, Nigella sativa, cardiotoxicity

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Author Biographies

Ikenna Kingsley Uchendu, Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Ebuka Bitrus Nnedu, Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Ifeoma Blessing Ekeigwe, Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

Department of Medical Laboratory Science, University of Nigeria, Enugu, Nigeria

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Uchendu IK, Nnedu EB, Ekeigwe IB. Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats. JDDT [Internet]. 15Feb.2020 [cited 4Jun.2020];10(1-s):174-7. Available from: http://jddtonline.info/index.php/jddt/article/view/3868