Efficacy of Aqueous Crude Fruit Extract of Desert Date (Balanites aegyptiaca) in Anaesthetization of African Catfish (Clarias gariepinus) Fingerlings

  • Idi Audu Wakawa Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria
  • Abubakar Chiroma Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria

Abstract

Chemicals have been used to anaesthetize fish but due to their hazardous effects on the environment, fish and humans environmentally friendly plant anaesthetics are being sought. Fruit of Desert date (Balanites aegyptiaca) is nontoxic to humans but has been reported to have anaesthetic potentials on fish. This study investigates anaesthetic effect of aqueous crude fruit extract of B. aegyptiaca on African catfish (Clarias gariepinus) fingerlings. Phytochemical and proximate compositions of the fruit were screened. Exactly 120 C. gariepinus fingerlings (mean weight 32.13±2.43g and mean total length 23.88±2.11cm) were used for the experiment. A total of 10 fingerlings were exposed to each of 2.00, 2.50, 3.00, 3.50 and 4.00g/L concentrations of aqueous crude fruit extract of B. aegyptiaca and a control in 6 plastic tanks (45x28x25cm) filled with 10L of tap water. Setup was arranged in randomized block design and replicated. Temperature, dissolved oxygen, pH, free carbon dioxide and total alkalinity were monitored. Results revealed long mean induction (25.05±3.35 min) and recovery (108.35±2.45 min) times with resultant mortalities (40%) of C. gariepinus fingerlings. significant correlation (P<0.01) exists between concentration of the fruit extract and induction time as well as between fruit extract concentrations and survival of fingerlings. Aqueous crude fruit extract of B. aegyptiaca is, therefore, not effective for anaesthetization of C. gariepinus fingerlings hence should be avoided.


Keywords: Anaesthetic, Balanites aegyptiaca, Clarias gariepinus

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Author Biographies

Idi Audu Wakawa, Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria

Chief Lecturer, Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria

Abubakar Chiroma, Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria

Chief Lecturer, Department of Biology, Umar Suleiman College of Education, Gashua, Yobe State, Nigeria

References

1. Neiffer DL, Stamper MA, Fish Sedation, Anaesthesia, Analgesia and Euthanasia: Considerations, Methods, and Types of Drugs. Institute for Laboratory Animal Research (ILAR), 2009; 50 (4): 343-360.
2. Javahery S, Moradlu AH, AQUI-S, New anaesthetic for use in fish propagation. Global Veterinaria, 2012; 9(2):205-210.
3. Matin SMA, Hossain MA, Hashim MA, Clove oil anesthesia in singhi (Heteropneuses fossilis) and Lata (Channa punctatus) fish. The Bangladesh Veterinarian, 2000; 26: 68-73.
4. Javahery S, Nechoubin H, Moradl AH, Effect of anaesthesia with clove oil in fish: A Review. Fish Physiology and Biochemistry, 2012; 38(6): 1545-1552.
5. Stuart NC, Anaesthesia in fish. Journal of Small Animal Practices. 1985; 22: 377-384.
6. Ramanayaka JC, Atapattu NSBM, Fish Anaesthetic Properties of Some Local Plant Materials. Tropical Agricultural Research & Extension, 2006; 9: 143-148.
7. Hajek G, Kłyszejko B, Dziaman R, The Anaesthetic effect of Clove Oil on Nile Tilapia, Oreochromis niloticus L. Acta Ichthyologica et Piscatoria, 2006; 36(2): 93–97.
8. Marking LL, Mayer F, Are better anaesthetics needed in fisheries? Fisheries, 1985; 10(6): 2-5
9. Kamble AD, Saini VP Ojha ML, The efficacy of clove oil as anesthetic in Nile tilapia (Oreochromis niloticus) and its potential metabolism reducing capacity. International Journal of Fauna and Biological Studies, 2014; 1(6):1-6
10. Chothani L. Vagshsiya HU. A review on Balanites aegyptiaca Del. (desert date):,phytochemical constituents, traditional uses, and pharmacological activity. Pharmacological Review, 2011; 5(9):55-62.
11. Onyema AM, Chinedu OJ, Ahmad MS, Evaluation of Balanites aegyptiaca Linna. Dlile, stem bark and synthetic surfactant for surface activity. American Journal of Chemistry and Application, 2017; 4(2):11-15.
12. Tesfaye, A. (2015). Balanites (Balanite aegyptiaca) Del., multipurpose tree: a prospective review. International Journal of Modern Chemistry and Applied Science, 2(3), 189-194.
13. Nkunya, M. H., Weenen, H., & Bray, D. H. (1990). Chemical evaluation of Tanzanian medicinal plants for the active constituents as a basis for the medicinal usefulness of the plants. In K. E. Mshigeni, M. H. Nkuanya, V. Fupi, R. L. Mahunnah, & E. N. Mshiu (Eds.). Proceedings of International Conference on Traditional Medicinal Plants. Arusha; pp.101–11.
14. Salihu M, Nwozo SO, Oloyede OB, Aqueous extract of Balanites aegyptiaca Del fruit mesocarp protects against CCL4 – induced liver damage in rats. British Journal of Pharmaceutical Research. 2013; 3(4): 917-928.
15. Absalom KV, Nwadiaro PO, Wophill, N, Toxicity of aqueous extract of desert date (B. aegyptiaca linnaeus) on the juveniles of catfish (Clarias gariepinus Teugels, 1986).Journal of Agriculture and Veterinary Science, 2013; 3(3):13-18.
16. Vijay P, Nivedita A, Bellundagi A, Ethanobaotany and qualitative phytochemical analysis of some Indian medicinal plants. International Journal of Chemical & Pharmaceutical Sciences, 2013; 4(2): 7-11.
17. Kumar KNS, Sangeetha B, Suchitra P, Ravishinkar B, Yashovarma B, Pharmacognosy and quality characterization of Balanites aegyptiaca (L.) Delile fruits. Indian Journal of Natural Products and Resources, 2016; 7(1): 40-50.
18. Umar KJ, Abubakar L, Alhassan B, Yahaya, SD, Hassan, LG, Sani NA, Nutritional profile of Balanites aegyptiaca flower. Vasile Goldis: University Press, 2014. P.169-173.
19. Ekpete OA, Edori OS, Fubara EP, Proximate composition and mineral composition of some Nigerian fruits. British Journal of Applied Science and Technology, 2013; 3(4):1447-1454.
20. Marcel A, Bievenu MJ, Proximate, mineral and phytochemical analysis of the leaves of H. myriantha and Urera trinervis. Pakistan Journal of Biological Sciences, 2012; 15(11): 536-541.
21. Emebu PK, Anyika JU, Proximate and mineral composition of Kale (Brassica oleracea) grown in Delta State, Nigeria. Pakistan Journal of Nutrition, 2011; 10:190-194.
22. Raimi MM, Oyekanmi AM, Farombi AG, Proximate and Phytochemical Composition of Leaves of Ceiba pentandra, Manihot esculentus and Abelmoschus esculentus in south western Nigeria. Scientific Research Journal, 2014; 2(4): 30-34.
23. Achi NK, Onyeabo C, Ekelem-Egedigwe CA, Onyeanula JC, Phytochemical, Proximate Analysis, Vitamin and Mineral Composition of Aqueous Extract of Ficus capensis leaves in South Eastern Nigeria. Journal of Applied Pharmaceutical Science, 2017; 7 (3):117-122.
24. Froese R, Daniel, P, (eds). Clarias gariepinus. In FishBase. 2014; March 2014 version. retrieved October 2, 2017 from https://en.wikipedia.org
25. Clay D, Population biology growth and feeding of Africa catfish (Clariasgariepinus) with special reference to juveniles and their importance in fish culture. Archipelago hydrobiology, 1989; 87(4):433 – 438.
26. Hamidu D, The management of water rights and its consequences in Bade Local Government Area of Yobe State. Unpublished master’s thesis, Bayero University, Kano, Kano; 2010; 155pp.
27. Trease GE, Evans WC, Pharmacognosy. 13th edition, Bailler Tindal, London 1989. P. 247-762.
28. Sofowora AF, Medicinal Plants and Traditional Medicine in Africa.. John Wiley and sons, New York, 1982, pp.256
29. Association of Official Analytical Chemist [AOAC], Official Methods of Analysis. (18th ed.), Washington. DC. 2006.
30. Audu BS, Omirinde JO, Gosomji IJ, Wazhi PE, Histopathological changes in the gill and liver of Clarias gariepinus exposed to acute concentrations of Vernonia amygdalina. Animal Research International, 2017; 14(1):2576-2587
31. Rezende FP, Pascoal LM, Vianna RA, Lanna EAT, Sedation of Nile tilapia with essential oils: tea tree, clove, eucleptus, and mint oils. Review of Caatinga, 2017; 30(2):479-486.
32. Summerfelt RC, Smith, LS, Methods for fish biology. In C. B. Schreck,, & P. B. Moyle, (Eds.) Anaesthesia, Surgery and Related Techniques. American Fisheries Society, Bethesda, MD; 1990. P. 213-272
33. American Public Health Association [APHA], Standard Methods for Examination of Water and Waste Water Washington, USA. 1988.
34. Tsuchiya H, Analgesic agents of plant origin: A review of phytochemicals with anaesthetic activity. Molecules, 2017; 22: 1369-1402
35. Olumiyiwa O, Babafemi J, Johnson C H, Patterns of Delay Amongst Pulmonary Tuberculosis Patient in Lagos. Bio-Medical Public Health, 2004; 4(18):147-245.
36. Okia CA, Agea JG, Kwetegyeka J, Okiror P, Kimondo J, Teklehaimanot Z, Nutritional value of commonly consumed desert date tree products. African Crop Science Journal, 2013; 21(3):657-667.
37. Wakawa A I, Audu BS, Sulaiman Y, Phytochemistry and proximate composition of root, stem bark, leaf and fruit of desert date, Balanites aegyptiaca. The Journal of Phytopharmacology, 2018;7(6): 464-470.
38. Ayoola PB, Adeyeye A, Proximate analysis and nutrient evaluation of some Nigerian pawpaw seeds varieties. Science Focus, 2009; 14(4):554-558.
39. Stehly GR, Gingerich WH, Evaluation of AQUI-STM (efficacy and minimum toxic concentration) as a fish anaesthetic/sedative for public aquaculture in the United States. Aquaculture Research, 1999; 30:365-372.
40. Audu BS, Adamu KM, Ufodike EBC, Behavioural response and opercula ventilation rate of Nile Tilapia (Oreochromis niloticus) fingerlings after anaesthesia with crude leaf extract of marijuana (Cannabis sativa). Applied Science Research Journal, 2013; 1(2): 66-77.
41. Anju TD, Solomon SG, Cheikyula JO, Effects of aqueous crude leaf extract of Tephropsia vogeli as a tranquilizer on the African Catfish, Hetreobranchus longifilis Val. (Pisces 1840). American Journal of Research Communication, 2015; 3(6):45-59.
42. Environmental Protection Agency (EPA), Fish physiology, toxicology and water quality. In: D, Randall, D Y M, Mandy (eds). Proceedings of the Eighth International Symposium, Chongqing, 2006; China between October 12th and 14th, 2004.
43. Mallya YJ, The effects of dissolved oxygen on fish growth in aquaculture. Final project, Kingolwira National Fish Farming Centre, Fisheries Division Ministry of Natural Resources and Tourism Tanzania. 2007.
44. Adebola K, Ayo F, Histological changes in liver, gills and kidney of catfish (Heterobranchus bidorsalis) exposed to cypermethrin concentration. International Journal of Histology and Cytology, 2014; 1(4):31-33.
45. Fafioye OO, Fagade SO, Adebisi AA, Toxicity of Raphia vinifera, P. beauv fruit extracts on biochemical composition of Nile tilapia (Oreochromis niloticus). Biokemistri, 2005; 17:137–142.
46. Wakawa A I, Audu BS, Sulaiman Y, Anaesthetic effects of aqueous crude leaf extracts of Desert date (Balanites aegyptiaca) on Nile tilapia Oreochromis niloticus) fingerlings. International Journal of Fisheries and Aquatic Studies, 2019; 7(1): 147-152.
47. Adebayo SF, Olufayo MO, Anaesthetic effects of Datura stramonium Leaf on Heterobranchus bidorsalis Juveniles. International Journal of Fisheries and Aquatic studies, 2017; 5(2):590-593.
48. Sladky KK, Swanson CR, Stockpot MK, Loomis, MR, Lewbart GA, Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthetics in Red Pacu (Piaractus brachypomus). American Journal of Veterinary Research, 2001; 62(3):337-342.
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Wakawa I, Chiroma A. Efficacy of Aqueous Crude Fruit Extract of Desert Date (Balanites aegyptiaca) in Anaesthetization of African Catfish (Clarias gariepinus) Fingerlings. JDDT [Internet]. 15Mar.2020 [cited 3Jul.2020];10(2):45-2. Available from: http://jddtonline.info/index.php/jddt/article/view/3859