Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice

  • Kokou Atchou Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo
  • Povi Lawson-Evi Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo
  • Kossi Metowogo Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo
  • Batomayena Bakoma Department of Pharmacy, Faculty of Health Sciences, University of Lome, Lome-Togo
  • Kwashie Eklu-Gadegbeku Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo
  • Kodjo AkliKokou Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo
  • Mensavi Gbeassor Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Abstract

Crataeva adansonii DC. ssp. adansonii is a medicinal plant belong to Capparidaceae family, used traditionally by Togolese to treat diabetes mellitus. The aim of this study was to evaluate the antihyperglycaemic and antioxidant activities of the plant leaves. The effect of hydroethanolic extract of C. adansonii ssp. adansonii leaves was assessed in vivo on blood glucose levels in fasting normal and glucose loaded hyperglycemic (oral glucose tolerance test) mice. The phytochemical screening has been done by coloring tests in chemistry. In vitro antioxidants assays had been performed by 2, 2'-azobis 2 amidinopropane dihydrochloride (AAPH) free radical scavenging assay, Ferric reducing antioxidant power (FRAP) assay, Fe2+ chelating assay and total antioxidant capacity (TAC) quantification. After 30 min glucose loading (4 g/kg), the extract 500 mg/kg was showed a significant (p < 0.001) decreased of the peak of blood glucose compared to the hyperglycemic control. The two doses of extract (250 and 500 mg/kg) did not act on the basic blood glucose compared to the normoglycemic control. Phytochemical screening revealed a presence of flavonoids, tannins, alkaloids and sterols. AAPH free radical scavenging activity, reducing power, Fe2+ chelating activity and total antioxidant capacity have shown dose-dependent antioxidant capacity and reducing power of extract compared to ascorbic acid and Butylated hydroxyl Toluene used as reference drug. These finding prove that C. adansonii ssp. adansonii leaves can be used in the treatment of diabetes mellitus and its complications related to oxidative stress and support the use of the plant in traditional medicine in diabetes treatment. 


Keywords: Crataeva adansonii, antihyperglycaemic, phytochemical, antioxidant.

Keywords: Crataeva adansonii, antihyperglycaemic, phytochemical, antioxidant.

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Author Biographies

Kokou Atchou, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Povi Lawson-Evi, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Kossi Metowogo, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Batomayena Bakoma, Department of Pharmacy, Faculty of Health Sciences, University of Lome, Lome-Togo

Department of Pharmacy, Faculty of Health Sciences, University of Lome, Lome-Togo

Kwashie Eklu-Gadegbeku, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Kodjo AkliKokou, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Mensavi Gbeassor, Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

Laboratory of Physiology-Pharmacology, Faculty of Sciences, University of Lome, Lome-Togo

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Atchou K, Lawson-Evi P, Metowogo K, Bakoma B, Eklu-Gadegbeku K, AkliKokou K, Gbeassor M. Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice. JDDT [Internet]. 15Feb.2020 [cited 12Jul.2020];10(1-s):30-8. Available from: http://jddtonline.info/index.php/jddt/article/view/3855