Phytochemical screening, Antimycobacterial activity of three medicinal Cameroonians plants and Acute toxicity of hydroethanolic extract of Vitellaria paradoxa

  • Jean Paul Assam Assam Department of Microbiology, Faculty of Science, University of Yaounde I P.O. Box 812, Yaounde, Cameroon
  • Marc Flaubert Yumsu Tcham Department of Biochemistry, Faculty of Science, University of Yaounde I P.O. Box 812, Yaounde, Cameroon
  • Ndedi Esther Del Florence Moni Department of Microbiology, Faculty of Science, University of Yaounde I P.O. Box 812, Yaounde, Cameroon
  • Diboue Patrick Hervé Betote 3 Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medecine and Biomedical Sciences, University of Yaounde I ; P.O. Box 812, Yaounde, Cameroon 4 Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaounde ; P.O. Box 13033, Yaounde, Cameroon
  • Tchinda Cedric Fossi Department of Biochemistry, Faculty of Science, University of Yaounde I P.O. Box 812, Yaounde, Cameroon
  • Beng Veronique Penlap Department of Biochemistry, Faculty of Science, University of Yaounde I P.O. Box 812, Yaounde, Cameroon


Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis (M. tuberculosis) complex, resposible  for health problems in developing countries. In Africa, various medicinal plants are traditionally used to treat  TB.  The aim of this study is to carry out the phytochemical screening, to evaluate the antimycobacterial activity of the crude extracts of three medicinal plants present in Cameroon (Zingiber officinale, Vitellaria paradoxa and Alstonia boonei) and the acute toxicity of hydroethanolic extract of Vitellaria paradoxa. The phytochemical screening was obtained by hydroethanolic extraction  and decoction. Inhibitory parameters of antimycobacterial activities were determined using the microplate alamar blue assay against M. tuberculosis H37Rv (ATCC 27294) and on one M. tuberculosis clinical strain. The crude extract with the best antimycobacterial activity was used for the acute toxicity assessment according to the OECD protocol. The results of the phytochemical screening revealed the presence of triterpenes and steroids in all the extracts, whereas  phenols were only present in the decoction of Alstonia boonei. All extracts tested showed antimycobacterial activities. The hydroethanolic extract of V. paradoxa  presented the best antimycobacterial activity with MICs of 78.13 and 625 μg/mL and MBCs of 78.13 and 2500 μg/mL respectively on M. tuberculosis H37Rv and on M. tuberculosis clinical strain. The results of the acute toxicity evaluation of V. paradoxa  showed a lethal dose 50  greater than 5000 mg/kg compared to control. The antimycobacterial activity of all the plant extracts used in this study justifies the traditional use of these medicinal plants on the treatment of  TB.

Keywords: Zingiber officinale, Vitellaria paradoxa, Alstonia boonei, Phytochemical screening, Antimycobacterial activity, Acute toxicity.

Keywords: Zingiber officinale, Vitellaria paradoxa, Alstonia boonei, Phytochemical screening, Antimycobacterial activity, Acute toxicity


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1- Baldwin, P.R., Reeves. A.Z., Powell, K.R., Napier, R.J., Swimm, A.I., Sun, A., Giesler, K., Bommarius, B., Shinnick, T.M., Sny–der, J.P., Liotta, D.C.,Monocarbonyl analogs of curcumin inhibit growth of antibiotic sensitive and resistant strains of Mycobacterium tuberculosis. European Journal of Medicinal Chemistry, 2015; 6 (92):693–9.
2- Zager, E.M., McNerney, R., Multidrug-resistant tuberculosis. BMC Infectious Diseases, 2008; 8:10.
3- Antony, M., James, J., Misra, C.S., Sagadevan, L.D.M., Veettil, A.T., Thankamani, V., Anti- mycobacterial activity of the plant extracts of Alstonia scholaris. International Journal of Current Pharmaceutical Research 2012; 4 (1):40 - 42.
4- Tang, J., Yam, WC., Chen, Z., Mycobacterium tuberculosis infection and vaccine development, 2016; 98:30 – 41.
5- WHO. Global tuberculosis report 2017. Licence: CC BY-NC-SA 3.0 IGO. ISBN 978 -92- 4 -156551- 6.
6- Ibekwe, N.N., Ameh, S.J., Plant natural products research in tuberculosis drug discovery and development: A situation report with focus on Nigerian biodiversity. African Journal of Biotechnology 2015; 13 (23), 2307 – 2320.
7- Proksch, P., Georgios, D., Rainer, K., Hendrik, K., Rudolf, H., Nicole, J., Victor, W., Wenhan, L., Callyaerins from the marine sponge Callyspongia aerizusa: cyclic peptides with antitubercular activity. Journal of Natural Products, 2015.
8- Gordien, A.Y., Gray, A.I., Franzblau, S.G., Seidel, V., Antimycobacterial terpenoids from Juniperus communis L. (Cuppressaceae). Journal of Ethnopharmacology, 2009; 126:500–505.
9- Nguta, J.M., Regina, A.O., Nyarko, A.K., Manu, D.Y., Addo, P.G.A., Current perspectives in drug discovery against tuberculosis from natural products. International Journal of Mycobacteriology, 2015b; 4:165– 183.
10- Abdullahi, M., Amupitan, J.O., Oyewale, A.O., Okogun, J.I., Kolo, I., An ethnobotanical survey of indigenous flora for treating tuberculosis and other respiratory diseases in Niger State, Nigeria. Journal Of Phytomedicine And Therapeutics; 2007; 12, 1-21.
11- Ogbole, O.O., Ajaiyeoba, E.O., Traditional management of tuberculosis in Ogun state of Nigeria: the practice and ethnobotanical survey. African Journal of Traditional, Complementary and Alternative Medicines, 2010; 7(1):79–84.
12- Aska, A.S., Kubmarawa, D., Preliminary Phytochemical Screening of Some Indigenous Medicinal Plants Used In the Treatment of Tuberculosis in Bauchi State, Nigeria. IOSR Journal of Applied Chemistry, 2016; 9 (4), 48-52.
13- Mutabana, N., Mpulusu, D., Plantes Médicinales cultivées dans la zone de Kabondo à Kisangani (Zaire). African Study Monographs, 1990; 11(2):87-99.
14- Ladoh-Y, C.F., Vandi, T., Dibong, S.D., Mpondo, M.E., Wansi, J.D., Betti, J.L., Choula, F., Ndongo, D., Tomedi, E.M., Étude ethnobotanique des plantes médicinales commercialisées dans les marchés de la ville de Douala, Cameroun. Journal of Applied Biosciences,2016; 99:9450–9466.
15- Ha, S.K., Moon, E., Ju, M.S., Kim, D.H., Ryu, J.H., Oh, M.S., Kim, S.Y., Shogaol, A Ginger Product, Modulates Neuroinflammation: A New Approach to Neuroprotection. Neuropharmacology, 2012; 63:211-23.
16- Haniadka, R., Saxena, A., Shivashankara, A.R., Fayad, R., Palatty, P.L., Nazreth, N., Francis, A., Arora, R., Baliga, M.S., Ginger Protects the Liver against the Toxic Effects of Xenobiotic Compounds: Preclinical Observations. Journal of Nutrition and Food Sciences 2013; 3:226.
17- Moustafa, B.H., Amal, K.M., Mohamed, E.R., Eman, H.G., A Review on Phenolic Compounds from Family Sapotaceae. Journal of Pharmacognosy and Phytochemistry 2016; 5(2):280-287.
18- Mpondo, M.E., Vandi, D., Ngouondjou, F.T., Mvogo, O.P.B., Embolo, E.E., Dibond, S.D., Contribution des populations des villages du centre Cameroun aux traitements traditionnels des affections des voies respiratoires. Journal of Animal & Plant Sciences 2017; 32(3):5223-5242.
19- Ballo, M.K., Etude phytochimique et l’évaluation de l’activité sur Mycobacterium tuberculosis in vitro de 22 plantes utilisées dans le traitement traditionnel de la tuberculose au Mali. Thèse de pharmacie, faculté de pharmacie. Université des sciences, des techniques et des technologies de Bamako, Mali, 2013.
20- Osadebe, P.O., Anti-infl ammatory properties of the root bark of Alstonia boonei. Nigerian Journal of Natural Products and Medicine 2002; 3:39– 41.
21- Prakash, P., Gupta, N., Thérapeutic uses of Ocimum sanctum (Tulsi) with a note on eugenol and its pharmacological actions : A Short Review. Indian Journal of Physiology and Pharmacology 2005; 49(2):125-131.
22- Sourabie, T.S., Nikiema, J.B., Guissou, I.P., Nacoulma, O.G., Etude comparée des effets anti-hépatotoxiques d’extraits d’Argemone mexicana L. (Papaveraceae), une plante utilisée dans le traitement traditionnel de la jaunisse au Burkina Faso. International Journal of Biological. Chemical Sciences, 2012; 6(3):1139-1147.
23- Rong X., Peng G., Suzuki T., Yang Q., Yamahara J et Li Y. A 35-day gavage safety assessment of ginger in rats.Regulatory Toxicology and Pharmacology, 2009; 54(2):118-123.
24- Harborne, J.B., Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. (3rd edition). Chapman and Hall Co., New York, 1998; Pp, 1-302.
25- Evans, W.C., Trease and Evans Pharmacology, 4th Edn. WB Saunders Company Ltd, 2000; Pp, 224- 239.
26- Salie, F., Eagles, P.F.K., Leng, H.M.J., Preliminary antimicrobial screening of four South African Asteraceae species. Journal of Ethnopharmacology 1996; 52:27–33.
27- Collins, L., Franzblau, S.G., Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrobial Agents and Chemotherapy, 1997; 41:1004–1009.
28- Carbonelle, B., Denis, F., Marmonier, A., Pinon, G., Vague, R., Bactériologie Médicale: Techniques Usuelles. SIMEP Edition, Paris, 1987; 228-282.
29- Gatsing, D., Mbah, J.A., Garba, I.H., Tane, P., Djemgou, P., Nji-Nkah, B.F., An Antisalmonellal Agent from the Leaves of Glossocalyx brevipes Benth (Monimiaceae). Pakistan Journal of Biological Sciences, 2006; 9:84-87.
30- OECD 423., OECD Guideline for testing of chemicals : Acute Oral Toxicity – Acute Toxic Class Method; 2001.
31- Mahmoudi, S., Khali, M., Mahmoudi, N., Etude de l’extraction des composés phénoliques de différentes parties de la fleur d’artichaut (Cynara scolymus L.). Revue « Nature & Technologie ». B- Sciences Agronomiques et Biologiques 2013; 9:35-40.
32- Lehout, R., Laib, M., Comparaison de trois méthodes d’extraction des composés phénoliques et des flavonoïdes à partir de la plante médicinale : Artemisia herba alba Asso. Mémoire de Master en Biochimie moléculaire et santé. Université des Frères Mentouri Constantine, Algérie. 2015; Pp, 41.
33- Kémajou, A., Mba, L., Bagda, A.A., Effet du séchage sur les principes actifs des plantes médicinales : cas des alcaloïdes totaux des écorces de Alstonia boonei Wild, plante antipaludéenne. Nature & Technology, 2012; 07 :62-66.
34- Mariangela, M., Francesco, M., Filomena, C., A comparative study of Zingiber officinale Roscoe pulp and peel: phytochemical composition and evaluation of antitumour activity. Natural Product Research, .2015; Pp, 3.
35- Bruneton, J., Pharmacognosie : Phytochimie, Plantes Médicinales. 3ème édition, Lavoisier Techniques & Documentation, Paris 1999; Pp, 231-236.
36- Kpemissi, A., Les anacardiaceae du togo : études botaniques, écologiques et propriétés antifongiques. Thèse de doctorat des Universités de Reims Champagne-Andenne et Lomé. Togo. 2007; Pp, 80-83.
37- Ross, C.F., Hoye, C.Jr., Fernandez-Plotka, V.C., Influence of Heating on the Polyphenolic Content and Antioxidant Activity of Grape Seed Flour. Journal of Food Science 2011; 76 :6.
38- Kobo, P.I., Erin, P.J., Suleiman, M.M., Aliyu, H., Tauheed, M., Muftau, S., Mamman, M., Antitrypanosomal effect of methanolic extract of Zingiber officinale (ginger) on Trypanosoma brucei brucei-infected Wistar mice. Veterinary World, 2014; 7(10) :770-775.
39- Amari, S., Étude phytochimique et évaluation de l’activité antibactérienne et antioxydante de deux extraits de la plante Zingiber officinale. Mémoire de master en Sciences biologiques, option-biochimie appliqué. Université Abou Bekr Belkaid-Tlemcen, Algérie. 2016.
40- Ogudo, B.U., Lawal, T.O., Adeniyi, B.A., Extracts of Zingiber officinale Rosc. (Ginger) and Curcuma longa Linn. (Turmeric) Rhizomes inhibited Nontuberculous Mycobacteria in vitro. Journal of Biology, Agriculture and Healthcare, 2014; 4 :12.
41- Voukeng, I.k., Penlap, B.V., Kuete, V., Antibacterial activity of six medicinal Cameroonian plants against Gram-positive and Gram-negative multidrug resistant phenotypes. BMC Complementary and Alternative Medicine, 2016; 16 :388.
42- Opoku, F., Akoto, O., Antimicrobial and Phytochemical Properties of Alstonia Boonei Extracts. Organic Chemistry Current Research 2015; 1 :137.
43- Owolabi, O.J., Arhewoh, I.M., Innih, S.O., Anaka, O.N., Monyei, C.F., The ethanol leaf extract of Alstonia boonei (Apocynaceae) reduces hyperglycemia in alloxan-induced diabetic rats. Nigerian Journal of Pharmaceutical Sciences 2014; 13(1) :12-21.
44- El-Mahmood, A.M., Doughari, J.H., Ladan, N., Antimicrobial screening of stem bark extracts of Vitellaria paradoxa against some enteric pathogenic microorganisms. African Journal of Pharmacy and Pharmacology 2008; 2(5):089-094.
45- Ndukwe, I.G., Amupitan, J.O., Isah, Y. and Adegoke, K.S., Phytochemical and antimicrobial screening of the crude extracts from the root, stem bark and leaves of Vitellaria paradoxa (GAERTN. F). African Journal of Biotechnology 2007; 6(16):1905-1909.
46- Fodouop, C.S.P., Tala, S.D., Lunga, K.P., Kodjio, N., Mefokou, Y.D., Nwabo, K.A.H., Nji-kah, B., Tchoumboue, J., Gatsing, D., Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats. BMC Complementary and Alternative Medicine, 2017; 17 :160.
47- Bruneton, J., Pharmacognosie : Phytochimie, Plantes Médicinales. 3ème édition, Lavoisier Techniques & Documentation, Paris, 1999; Pp, 231-236.
48- Nyegue, M.A., Propriétés chimiques et biologiques des huiles essentielles de quelque plantes aromatiques et/ou médicinales du Cameroun: évaluation de leurs activités antiradicalaires, antiinflammatoires et antimicrobiennes. Thèse de doctorat des universités Montpellier II et Yaoundé I. Cameroun 2006; Pp, 46-143
49- Hudaib, M., Speroni, E., Di Pietra, A.M., Cavrini, V., GC/ MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle. Journal of Pharmaceutical and Biomedical and Analysis 2002; 29 :691-700.
50- Nguta Joseph. M., Appiah-Opong Regina., Nyarko Alexander. K., Yeboah-Manu Dorothy., Addo Phyllis. G. A., Otchere Isaac., Kissi-Twum Abena. Antimycobacterial and cytotoxic activity of selected medicinal plant extracts. Journal of Ethnopharmacology, 2016; 182:10 – 15
51- Paiva, P.M.G., Gomes, F.S., Napoleão, T.H., R.A. Sá R. A., Correia, M.T.S., Coelho, L.C.B.B., Antimicrobial activity of secondary metabolites and lectins from plants. A. Méndez-vilas (Ed) 2010.
52- Cowan, M.M., Plant products as antimicrobial agents. Clinical Microbiology Reviews 1999; 12:564-582.
53- Thangara, J.H.S., Adjei, O., Allen, B.W., Portaels, F., In-vitro activity of ciprofloxacin, sparfloxacin, ofloxacin, amikacin and rifampicin against Ghanian isolates of Mycobacterium ulcerans. Journal Antimicrobial Agents Chemoter 2000; 45 (2) :231-233.
54- Bhimba, B.V., Meenupriya, J., Lycias, J.E., Naveena, D.E., kumar, S., Thangaraj, M., Antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis. Asian Pacific Journal of Tropical Medicine 2010; Pp, 412-420.
55- Kuete, V., Potential of Cameroonian plants and derived products against microbial infections : A Review. Planta Medica 2010; 76 :1479–1491.
56- Gordien Andréa Y., Gray Alexander I., Franzblau Scott G., Seidel Véronique. Antimycobacterial terpenoids from Juniperus communis L. (Cuppressaceae). Journal of Ethnopharmacology 2009; 126:500–505
57- Brent Copp R. Antimycobacterial natural products. Nat. Prod. Rep 2003; 20:535–557
58- Kuete, V., Tangmouo, J.G., Meyer, M.J.J., Lall, N. Diospyrone, crassiflorone and plumbagin: three antimycobacterial and antigonorrhoeal naphthoquinones from two Diospyros spp. International Journal of Antimicrobial Agents, 2009; 34 :322–325.
59- Hiserodt, R.D., Franzblau, S.G., Rosen, R.T. Isolation of 6-, 8-, and 10-Gingerol from Ginger Rhizome by HPLC and Preliminary Evaluation of Inhibition of Mycobacterium avium and Mycobacterium tuberculosis. Journal of Agricultural and Food Chemistry, 1998; 46, 2504-08.
60- Raju, A., Degani, M.S., Khambete, M.P., Ray, M.K., Rajan, M.G.R. Antifolate activity of plant polyphenols against Mycobacterium tuberculosis. Phytotherapy Research 2015; 29 :1646-1651.
61- Adotey, K.J.P., Adukpo, E.G., Boahen, O.Y., Armah, A.F., A Review of the Ethnobotany and Pharmacological Importance of Alstonia boonei De Wild (Apocynaceae). International Scholarly Research Network Pharmacology 2012; P, 9.
62- OECD. Harmonised Integrated Hazard Classification for Human Health and Environmental Effects of Chemical Substances as endorsed by the 28th Joint Meeting of the Chemicals Committee and the Working Party on Chemicals in November 1998, Part 2, P.11. [,3380,EN-documents-521-14-no-24-no-0,FF.html]. (Consulté le 4 Avril 2018).
63- Mainasara, A.S., Oduola, T., Musa, U., Mshelia, A.S., Muhammed, A.O., Ajayi, A.S., . Hepatotoxicity Assessment in Wistar Rats Exposed to Vitellaria paradoxa Stem Bark Extract. European Journal of Medicinal Plants 2016a;13(3):1-9.
64- Mainasara, A.S., Oduola, T., Musa, U., Mshelia, A.S., Muhammed, A.O., Ajayi, A.S., Effect of Vitellaria paradoxa Stem Bark Ingestion on Kidney Functions in Wistar Rats. British Journal of Pharmaceutical Research 2016b ;11(2):1-8.
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Assam Assam JP, Yumsu Tcham MF, Moni NEDF, Betote DPH, Fossi TC, Penlap BV. Phytochemical screening, Antimycobacterial activity of three medicinal Cameroonians plants and Acute toxicity of hydroethanolic extract of Vitellaria paradoxa. JDDT [Internet]. 15Feb.2020 [cited 27Feb.2020];10(1-s):96-04. Available from: