Ligand Decorated Embelin Loaded PLGA Nanoparticles for Management of Alcohol Induced Hepatotoxicity
Preparation of surface modified Embelin loaded nanoparticles (GA-PEG-PLGA) for the management of hepatotoxicity. Surface modified Embelin loaded GA-PEG-PLGA NPs were evaluated by NMR, FTIR, TEM techniques and in vitro release studies. The biodistribution of the nanoparticles was assessed by High-performance liquid chromatography (HPLC), and the cellular uptake study was evaluated using Hep G2 cells (liver cells lines). The hepatoprotecttive effect of the surface modified Embelin loaded GA-PEG-PLGA NPs was investigated in-vitro and in-vivo. The surface modified Embelin loaded GA-PEG-PLGA nanoparticles significantly increases the uptake of drug in liver by 2.5 folds more than plain drug.
Keywords: Glycyrrhetinic acid, Receptor mediated, Surface functionalization, Embelin, PLGA nanoparticle.
2. Moghimi SM, Hunter AC, Murray JC. Long-circulating and target-specific nanoparticles: theory to practice. Pharmacological reviews. 2001;53(2):283-318.
3. Brannon-Peppas L, Blanchette JO. Nanoparticle and targeted systems for cancer therapy. Advanced drug delivery reviews. 2012;64:206-12.
4. Gu FX, Karnik R, Wang AZ, Alexis F, Levy-Nissenbaum E, Hong S, et al. Targeted nanoparticles for cancer therapy. Nano today. 2007;2(3):14-21.
5. Poojari R, Gupta S, Maru G, Khade B, Bhagwat S. Chemopreventive and hepatoprotective effects of embelin on N-nitrosodiethylamine and carbon tetrachloride induced preneoplasia and toxicity in rat liver. Asian Pac J Cancer Prev. 2010;11(4):1015-20.
6. Kothavade R, Joglekar S, Barodavalla S. Protective effect of an indigenous drug livomyn on ketoconazole induced hepatotoxicity. Indian journal of pharmaceutical sciences. 1996;58(4):142.
7. Gupta O, Ali MM, Ray GB, Atal C. Some pharmacological investigations of embelin and its semisynthetic derivatives. Indian journal of physiology and pharmacology. 1976;21(1):31-9.
8. Ghosh N, Ghosh R, Mandal V, Mandal SC. Recent advances in herbal medicine for treatment of liver diseases. Pharmaceutical Biology. 2011;49(9):970-88.
9. Singh D, Singh R, Singh P, Gupta RS. Effects of embelin on lipid peroxidation and free radical scavenging activity against liver damage in rats. Basic & clinical pharmacology & toxicology. 2009;105(4):243-8.
10. Kawakami S, Munakata C, Fumoto S, Yamashita F, Hashida M. Novel galactosylated liposomes for hepatocyte‐selective targeting of lipophilic drugs. Journal of pharmaceutical sciences. 2001;90(2):105-13.
11. Sato A, Kawakami S, Yamada M, Yamashita F, Hashida M. Enhanced gene transfection in macrophages using mannosylated cationic liposome-polyethylenimine-plasmid DNA complexes. Journal of drug targeting. 2001;9(3):201-7.
12. Huang W, Wang W, Wang P, Tian Q, Zhang C, Wang C, et al. Glycyrrhetinic acid-modified poly (ethylene glycol)–b-poly (γ-benzyl l-glutamate) micelles for liver targeting therapy. Acta biomaterialia. 2010;6(10):3927-35.
13. Onishi H, Takahashi H, Machida Y. Preparation and evaluation of glycyrrhetic acid‐containing microparticles as an anti‐hepatotoxic system. Drug development research. 2005;66(3):189-99.
14. Zhi Y, Huang W, Tian Q, Zhang C, Han L, Liu T, et al. Glycyrrhetinic acid-mediated nanoparticles of hepatic targeted drug delivery system, process for preparing the same and use thereof. Google Patents; 2015.
15. Patil YB, Toti US, Khdair A, Ma L, Panyam J. Single-step surface functionalization of polymeric nanoparticles for targeted drug delivery. Biomaterials. 2009;30(5):859-66.
16. Zhao H, Yung LYL. Selectivity of folate conjugated polymer micelles against different tumor cells. International journal of pharmaceutics. 2008;349(1):256-68.
17. Nordström P. Formation of polymeric nanoparticles encapsulating and releasing a new hydrophobic cancer drug. 2011.
18. Kara A, Ozturk N, Sarisozen C, Vural I. Investigation of formulation parameters of plga nanoparticles prepared by nanoprecipitation technique. 2014.
19. Hornig S, Heinze T, Becer CR, Schubert US. Synthetic polymeric nanoparticles by nanoprecipitation. Journal of Materials Chemistry. 2009;19(23):3838-40.
20. Zhang L, Zhang L. Lipid–polymer hybrid nanoparticles: synthesis, characterization and applications. Nano Life. 2010;1(01n02):163-73.
21. Bradley D, Maynard J, Emery G, Webster H. Transaminase activities in serum of long-term hemodialysis patients. Clinical chemistry. 1972;18(11):1442-.
22. Lieberman D, Phillips D. " Isolated" elevation of alkaline phosphatase: significance in hospitalized patients. Journal of clinical gastroenterology. 1990;12(4):415-9.
23. Jendrassik L, Grof P. Simplified photometric methods for the determination of the blood bilirubin. Biochem Z. 1938;297(81):b5.
24. Song X, Zhao Y, Hou S, Xu F, Zhao R, He J, et al. Dual agents loaded PLGA nanoparticles: systematic study of particle size and drug entrapment efficiency. European journal of pharmaceutics and biopharmaceutics. 2008;69(2):445-53.
25. Yadav AK, Mishra P, Mishra AK, Mishra P, Jain S, Agrawal GP. Development and characterization of hyaluronic acid–anchored PLGA nanoparticulate carriers of doxorubicin. Nanomedicine: Nanotechnology, Biology and Medicine. 2007;3(4):246-57.
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